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Identification and characterization of a bacteriocin produced by an isolated Bacillus sp. SW1-1 that exhibits antibacterial activity against fish pathogens

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Abstract

The selected isolate, Bacillus sp. SW1-1 showed antibacterial activity against both Gram-positive and Gram-negative bacteria involved in fish diseases, including Edwardsiella tarda, Streptococcus iniae, S. parauberis, Vibrio anguillarum, and V. harveyi. The Maximum bacteriocin production was observed at 30°C after 24 h with brain heart infusion medium (pH 7.0). The bacteriocin SW1-1 was purified by 50% ammonium sulfate precipitation, followed by HiPrep diethylaminoethyl 16/10 FF and Sephacryl S-100 High resolution column chromatography. The substance was characterized as a bacteriocin-like inhibitory substance with a molecular mass of 38 kDa. Bacteriocin SW1-1 was sensitive to the proteolytic action of pepsin, trypsin, chymotrypsin, and protease types I and XIV, and relatively heat labile, despite the fact that bacteriocin activity was still detected after heating at 100°C for 30 min. The activity of bacteriocin SW1-1 was stable in the pH range of 2.0–11.0, and relatively unaffected by organic chemicals. The bacteriocin SW1-1 had a bacteriolytic mechanism, resulting in cell wall degradation of E. tarda. These characteristics indicate that this bacteriocin may be a potential candidate for alternative agent to control important pathogens of fish diseases in aquaculture.

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Authors and Affiliations

  1. Biotechnology Research Division, National Fisheries Research and Development Institute, 216 Gijanghaean-ro, Gijang, Busan, 619-705, Republic of Korea

    Young-Ok Kim, In-Suk Park, Bo-Hye Nam, Dong-Gyun Kim, Young-Ju Jee & Cheul-Min An

  2. New Strategy Research Center, National Fisheries Research and Development Institute, Busan, 619-705, Republic of Korea

    Dae-Jung Kim

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  1. Young-Ok Kim
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  2. In-Suk Park
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  3. Dae-Jung Kim
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  4. Bo-Hye Nam
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  5. Dong-Gyun Kim
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  6. Young-Ju Jee
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  7. Cheul-Min An
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Kim, YO., Park, IS., Kim, DJ. et al. Identification and characterization of a bacteriocin produced by an isolated Bacillus sp. SW1-1 that exhibits antibacterial activity against fish pathogens. J Korean Soc Appl Biol Chem 57, 605–612 (2014). https://doi.org/10.1007/s13765-014-4174-1

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  • DOI: https://doi.org/10.1007/s13765-014-4174-1

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