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Genetic Suppressor Elements in the Characterization and Identification of Tumor Suppressor Genes

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Tumor Suppressor Genes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 222))

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Abstract

The subject of the present chapter is the genetic suppressor element (GSE) methodology, a functional genomics platform for identifying and characterizing genes involved in different cellular phenotypes, and the applications of this methodology to the study of tumor suppressor genes (TSG). In this overview, we will first describe the principles and basic elements of GSE selection and then concentrate on the methods and examples for using this methodology to identify and analyze TSGs. For detailed “cookbook” protocols of methods used in GSE selection, we refer the reader to previously published methodologic works (1,2).

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Author information

Authors and Affiliations

  1. Department of Molecular Genetics, University of Illinois, Chicago, IL

    Igor B. Roninson

  2. Department of Molecular Biology, Lerner Research Institute Cleveland Clinic Foundation, Cleveland, OH

    Andrei V. Gudkov

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  1. Igor B. Roninson
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  2. Andrei V. Gudkov
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Editors and Affiliations

  1. Departments of Medicine, Genetics, and Pharmacology, University of Pennsylvania School of Medicine, Philadelphia, PA

    Wafik S. El-Deiry MD, PhD

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© 2003 Humana Press Inc.

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Roninson, I.B., Gudkov, A.V. (2003). Genetic Suppressor Elements in the Characterization and Identification of Tumor Suppressor Genes. In: El-Deiry, W.S. (eds) Tumor Suppressor Genes. Methods in Molecular Biology™, vol 222. Humana Press, Totowa, NJ. https://doi.org/10.1385/1-59259-328-3:413

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  • DOI: https://doi.org/10.1385/1-59259-328-3:413

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-986-5

  • Online ISBN: 978-1-59259-328-6

  • eBook Packages: Springer Protocols

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