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Identification and Fine Mapping of Candidate Gene for Yellow Leaf Mutant (ygl54) Exhibiting Yellow Leaf Colour in Rice

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Abstract

Yellow leaf mutants are useful genetic variants to explore the mechanism of chloroplast development. In this study, we developed and characterized a new rice (Orzya sativa L.) yellow leaf mutant, ygl54, with ethylmethane sulfonate (EMS) treatment on Zhenong 34, which exhibited leaf yellowing at early stage. By map-based cloning, OsYGL54 was located on long arm of chromosome 11 with a physical distance of 26 kb, and the substitution from G to A at the first exon of LOC_Os11g36440 was identified. Silent mutation in subsequent codon of ATG, disturbed the translation initiation, which caused lower expression level of OsYGL54 across the tissues. The histological analysis revealed that the chloroplast of mesophyll cells in ygl54 was disrupted with reduction in size of starch granules and higher number of plastoglobuli, which resulted in decreased chlorophyll contents, lower photosynthetic parameter, and lower expression level of genes associated with pigment metabolism and photosynthesis. The disruption in chloroplast caused higher accumulation of H2O2 and MDA contents. In conclusion, our results revealed the importance of sequence of second codon suggests that OsYGL54 was possibly involved in pigment metabolism and valuable for further study to explore the mechanism of chloroplast development.

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Funding

This work was supported by the National Key Research and Development of China (project no. 2017YFD0100305) and the Science and Technology Office of Zhejiang Province, China (project no. 2016C02050-6).

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Authors and Affiliations

  1. Department of Agronomy, and the Key Laboratory for Crop Germplasm Resource of Zhejiang Province, College of Agriculture and Biotechnology, Zhejiang University, 310058, Hangzhou, China

    A. Khan, S. Jalil, H. Cao, M. Sunusi, Y. Tsago, J. Chen, C. H. Shi & X. L. Jin

Authors
  1. A. Khan
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  2. S. Jalil
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  3. H. Cao
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  4. M. Sunusi
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  5. Y. Tsago
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  6. J. Chen
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  7. C. H. Shi
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  8. X. L. Jin
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Contributions

A.K. and X.J. developed and designed the experiments. A.K. conducted the main experiments and wrote the manuscript. C.H.S identified and provided the populations of the ygl54 mutant for the genetic study and carried out the gene mapping. S.J., H.C., M.S., Y.T., Z.C. and J.C. helped to carry out the experiments. All authors have read and approved the final manuscript.

Corresponding author

Correspondence to X. L. Jin.

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Conflict of interests. The authors declare that they have no conflicts of interest.

Statement on the welfare of humans or animals. This article does not contain any studies involving animals performed by any of the authors.

Additional information

Abbreviations: APX—ascorbate peroxidase; EMS—ethylmethane sulfonate; POD—peroxidase; ROS—reactive oxygen species; SOD— superoxide dismutase; TEM—transmission electron microscope; WT—wild type plants.

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Khan, A., Jalil, S., Cao, H. et al. Identification and Fine Mapping of Candidate Gene for Yellow Leaf Mutant (ygl54) Exhibiting Yellow Leaf Colour in Rice. Russ J Plant Physiol 68, 1069–1078 (2021). https://doi.org/10.1134/S1021443721060078

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