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Identification of Mitogen-activated Protein Kinase from Schizochytrium sp. and Application in Resisting Stress Environments

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Abstract

Schizochytrium sp. is a marine fungus, which usually lives in the salinity of 40–100% seawater and has unique salt tolerance characteristics. In this study, we explored the candidate mitogen-activated protein kinase (MAPK) related to the salt-tolerant regulatory network from Schizochytrium sp. Firstly, 6 potential MAPK genes from Schizochytrium sp. were identified based on the genome information and bioinformatics analysis. Then, the MAPK gene expression of Schizochytrium sp. under different cultivation conditions locked two genes encoding Mpk6315 and Mpk2022. Furthermore, overexpressing of Mpk6315 in S. cerevisiae enhanced cell growth by 62% while and Mpk2022 overexpression improved the membrane integrity under different stress conditions, indicating that Mpk6315 might play an important role for cell growth and Mpk2022 could help cells to encounter different environmental stressors. This study was the first time to identify the MAPK genes from Schizochytrium sp., which could enlarge the MAPK modules of salt stress tolerance and provide new elements for improving the stress resistance of other microorganisms.

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DATA AVAILABILITY STATEMENTS

The authors declare that the data supporting the findings of this study are available within the article and its supplementary information files.

SUPPLIMENTARY MATERIALS

Fig. S1 . Plasmid maps. (A) A2022 MAPK pESC-Leu map; (B) A6315 MAPK pESC-Leu map.

Fig. S2 . Agarose gel electrophoresis of pESC-Mpk2022 and pESC-Mpk6315 plasmid construction: (A) Colony PCR of E.coli DH5α containing pESC-Mpk2022 and pESC- Mpk6315 plasmid; (B) Colony PCR of S. cerevisiae yph499 containing pESC- Mpk2022 and pESC- Mpk6315 plasmid.

Fig. S3 . Mpk2022 and Mpk6325 genes of the MAPK pathway is involved in conditional stresses response to cell injury in S. cerevisiae (A) Cell membrane damages of control strain pESC and overexpression strain Mpk2022, Mpk6315 under normal conditions; (B) Cell membrane damages under 0.5 M NaCl; (C) Cell membrane damages under 1 M NaCl; (D) Cell membrane damages under 8% ethanol; (E) Cell membrane damages under 10% ethanol; (F) Cell membrane damages under 37°C; (G) Cell membrane damages under 42°C.

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ACKNOWLEDGMENTS

This work was financially supported by the National Key R&D Program of China (no.: 2019YFA0905700), the National Natural Science Foundation of China (no. 21878151), the Natural Science Foundation of Jiangsu Province (BK20211535) and the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture (XTD2213).

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  1. College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech. University, 211816, Nanjing, People’s Republic of China

    C. Du, J. Jiang, X. Hu & L. Ren

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  1. C. Du
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Correspondence to L. Ren.

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Du, C., Jiang, J., Hu, X. et al. Identification of Mitogen-activated Protein Kinase from Schizochytrium sp. and Application in Resisting Stress Environments. Appl Biochem Microbiol 59, 438–449 (2023). https://doi.org/10.1134/S000368382304004X

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