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Fungal diversity in the Antarctic active layer

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Abstract

Taxonomic diversity of fungi in the samples of the active layer of Antarctica was investigated using conventional microbiological techniques and metagenomic analysis of total DNA extracted from environmental samples. The list of Antarctic microscopic fungi was expanded, including detection of the species representing a portion of the fungal complex which is nonculturable or sterile on conventional nutrient media.

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References

  1. Vishniak, H.S., The microbiology of Antarctic soils, in Antarctic Microbiology, Friedmann, E.I., Ed., New York: Willey-Liss, 1993.

    Google Scholar 

  2. Onofri, S., Selbmann, L., Zucconi, L., Tosi, S., Fenice, M., Barreca, D., and Ruisi, S., Studies on Antarctic fungi, Polarnet Tech. Rep., 2005, vol. 1, pp. 49–52.

    Google Scholar 

  3. Ruisi, S., Berreca, D., Selbmann, L., Zucconi, L., and Onofri, S., Fungi in Antarctica, Rev. Environ. Sci. Biotechnol., 2007, vol. 6, pp. 127–141.

    Article  Google Scholar 

  4. Kochkina, G.A., Ivanushkina, N.E., Karasev, S.G., Gavrish, E.Yu., Gurina, L.V, Evtushenko, L.I., Spirina, E.V., Vorob’eva, E.A., Gilichinskii, D.A., and Ozerskaya, S.M., Survival of micromycetes and actinobacteria under conditions of long-term natural cryopreservation, Microbiology (Moscow), 2001, vol. 70, no. 3, pp. 356–364.

    Article  CAS  Google Scholar 

  5. Gilichinsky, D.A., Wilson, G.S., Friedmann, E.I., McKay, C.P., Sletten, R.S., Rivkina, E.M., Vishnivetskaya, T.A., Erokhina, L.G., Ivanushkina, N.E., Kochkina, G.A., Shcherbakova, V.A., Soina, V.S., Spirina, E.V., Vorobyova, E.A., Fyodorov-Davydov, D.G., Halle, B., Ozerskaya, S.M., Sorokovikov, V.A., Laurinavichyus, K.S., Shatilovich, A.V., Chanton, J.P., Ostroumov, V.E., and Tiedje, J.M., Microbial populations in Antarctic permafrost: biodiversity, state, age and implication for astrobiology, Astrobiology, 2007, vol. 2, pp. 275–311.

    Article  Google Scholar 

  6. Onofri, S., Zucconi, L., Selbmann, L., Ripa, C., and Frisvad, J.C., Searching for eukaryotic life preserved in Antarctic permafrost, in Mat. 38th. COSPAR Sci. Ass., Bremen, 2010, p. 9.

    Google Scholar 

  7. Tosi, S., Casado, B., Gerdol, R., and Caretta, G., Fungi isolated from Antarctic mosses, Polar Biol., 2002, vol. 25, pp. 262–268.

    Google Scholar 

  8. Vishniac, H.S., Biodiversity of yeasts and filamentous microfungi in terrestrial Antarctic ecosystems, Biodiv. Conservation, 1996, vol. 5, pp. 1365–1378.

    Article  Google Scholar 

  9. Kozlovsky, A.G., Zhelifonova, V.P., Antipova, T.V., Adanin, V.M., Ozerskaya, S.M., Ivanushkina, N.E., Gollmick, F.A., and Gräfe, U., A new N-carboxylester derivative of 3,12-dihydroroquefortine, Heterocycles, 2003, vol. 60, no. 7, pp. 1639–1644.

    Article  CAS  Google Scholar 

  10. Smith, J.J., Tow, L.A., Stafford, W., Cary, C., and Cowan, D.A., Bacterial diversity in three different Antarctic cold desert mineral soils, Microb. Ecol., 2006, vol. 51, pp. 413–421.

    Article  PubMed  Google Scholar 

  11. Babalola, O.O., Kirby, B.M., Le Roes-Hill, M., Cook, A.E., Cary, S.C., Burton, S.G., and Cowan, D.A., Phylogenetic analysis of actinobacterial populations associated with Antarctic Dry Valley mineral soils, Environ. Microbiol., 2009, vol. 11, pp. 566–576.

    Article  CAS  PubMed  Google Scholar 

  12. Malosso, E., Waite, I.S., English, L., Hopkins, D.W., and O’Donnell, A.G., Fungal diversity in maritime Antarctic soils determined using a combination of culture isolation, molecular fingerprinting and cloning techniques, Polar Biol., 2006, vol. 29, no. 7, pp. 552–561.

    Article  Google Scholar 

  13. Margesin, R. and Miteva, V., Diversity and ecology of psychrophilic microorganisms, Res. Microbiol., 2011, vol. 162, pp. 346–361.

    Article  PubMed  Google Scholar 

  14. Kochkina, G., Ivanushkina, N., Ozerskaya, S., Chigineva, N., Vasilenko, O., Firsov, S., Spirina, E., and Gilichinsky, D., Ancient fungi in Antarctic permafrost environments, FEMS Microbiol. Ecol., 2012, vol. 82, no. 2, pp. 501–509.

    Article  CAS  PubMed  Google Scholar 

  15. Boerema, G.H., De Gruyer, J., Noordeloos, M.E., and Hamers, M.E.C., Phoma Identification Manual, CABI Publishing, 2004.

    Google Scholar 

  16. van Oorschot, C.A.N., A revision of Chrysosporium and allied genera, Stud. Mycol., 1980, vol. 20, pp. 1–89.

    Google Scholar 

  17. Frisvad, J. and Samson, R.A., Penicillium subgenus Penicillium: new taxonomic schemes, mycotoxins and other extrolites, Stud. Mycol., 2004, vol. 49, pp. 1–260.

    Google Scholar 

  18. White, T.J., Bruns, T., Lee, S., and Taylor, J., Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics, in PCR Protocols: A Guide to Methods and Applications, Innis, M.A., Gelfand, D.H., Sninsky, J.J., and White, T.J., Eds., San Diego: Academic, 1990, pp. 315–322.

    Google Scholar 

  19. Elgar, R.C., Haas, B.J., Clemente, J.C., Quince, C., and Knight, R., UCHIME improves sensitivity and speed of chimera detection, Bioinformatics, 2011, vol. 27, no. 16, pp. 2194–2200.

    Article  Google Scholar 

  20. Duncan, S.M., Fungal diversity and cellulytic activity in the Historic Huts, Ross Island, Antarctica, PhD Thesis, Univ. Waikato, 2007.

    Google Scholar 

  21. De Hoog, S., Zalar, P., van der Ende, B.G., and Gunde-Cimerman, N., Relation of halotolerance to human-pathogenicity in the fungal tree of life: an overview of ecology and evolution under stress, in Adaptation to Life at High Salt Concentrations in Archaea, Bacteria, and Eukarya, Gunde-Cimerman, N., Oren, A., and Plementtas, A., Eds., Springer, 2005, pp. 371–395.

    Chapter  Google Scholar 

  22. Ivanushkina, N.E., Kochkina, G.A., and Ozerskaya, S.M., Fungi in ancient permafrost sediments of the Arctic and Antarctic regions, in Life in Ancient Ice, Castello, J. and Rogers, S., Eds., Ch. 9, Princeton: Princ. Univ. Press, 2005, pp. 127–139.

    Google Scholar 

  23. Sogonov, M.V., Schroers, H.J., Gams, W., Dijksterhuis, J., and Summerbell, R.C., The hyphomycete Teberdinia hygrophila gen. nov., sp. nov. and related anamorphs of Pseudeurotium species, Mycologia, 2005, vol. 97, no. 3, pp. 695–709.

    Article  CAS  PubMed  Google Scholar 

  24. Schneider, M., Grunig, C.R., Holdenrieder, O., and Sieber, T.N., Cryptic speciation and community structure of Herpotrichia juniperi, the causal agent of brown felt blight of conifers, Mycol. Res., 2009, vol. 113, no. 8, pp. 887–896.

    Article  PubMed  Google Scholar 

  25. Gessner, M.O. and Robinson, C.T., Aquatic hyphomycetes in alpine streams, in Ecology of a Glacial Flood Plain, Ward, J.V. and Uehlinger, U., Eds., Kluwer: Dordrecht, 2003, pp. 123–137.

    Chapter  Google Scholar 

  26. Stchigel, A.M., Cano, J., MacCormack, W., and Guarro, J., Antarctomyces psychrotrophicus gen. et sp. nov., a new ascomycete from Antarctica, Mycolog. Res., 2001, vol. 105, no. 3, pp. 377–382.

    Article  CAS  Google Scholar 

  27. Xiao, N., Suzuki, K., Nishimiya, Y., Kondo, H., Miura, A., Tsuda, S., and Hoshino, T., Comparison of functional properties of two fungal antifreeze proteins from Antarctomyces psychrotrophicus and Typhula ishikariensis, FEBS J., 2010, vol. 277, no. 2, pp. 394–403.

    Article  CAS  PubMed  Google Scholar 

  28. Engblom, E., Lingdell, P., Marvanova, L., and MullerHaeckel, A., Foam spora in running waters of Southern Greenland, Polar Res., 1986, vol. 4, no. 1, pp. 47–51.

    Article  Google Scholar 

  29. Bridge, P.D. and Newsham, K.K., Soil fungal community composition at Mars Oasis, a southern maritime Antarctic site, assessed by PCR amplification and cloning, Fungal Ecol., 2009, vol. 2, no. 2, pp. 66–74.

    Article  Google Scholar 

  30. Zhurbenko, M.P., Lichenophilic fungi of Russian Arctics, Extended Abstract of Doctoral (Biol.) Dissertation, St.-Petersburg, Komarov Botanical Inst., Russ. Acad. Sci., 2010.

    Google Scholar 

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Authors and Affiliations

  1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia

    G. A. Kochkina, S. M. Ozerskaya, N. E. Ivanushkina, N. I. Chigineva & O. V. Vasilenko

  2. Institute of Physicochemical and Biological Problems of Soil Science, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia

    E. V. Spirina & D. A. Gilichinskii

Authors
  1. G. A. Kochkina
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  2. S. M. Ozerskaya
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  3. N. E. Ivanushkina
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  4. N. I. Chigineva
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  5. O. V. Vasilenko
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  6. E. V. Spirina
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  7. D. A. Gilichinskii
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Corresponding author

Correspondence to G. A. Kochkina.

Additional information

Original Russian Text © G.A. Kochkina, S.M. Ozerskaya, N.E. Ivanushkina, N.I. Chigineva, O.V. Vasilenko, E.V. Spirina, D.A. Gilichinskii, 2014, published in Mikrobiologiya, 2014, Vol. 83, No. 2, pp. 236–244.

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Kochkina, G.A., Ozerskaya, S.M., Ivanushkina, N.E. et al. Fungal diversity in the Antarctic active layer. Microbiology 83, 94–101 (2014). https://doi.org/10.1134/S002626171402012X

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  • DOI: https://doi.org/10.1134/S002626171402012X

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