Skip to main content
SpringerLink
Log in

Photorepair activity and protective compounds in two freshwater zooplankton species (Daphnia menucoensis and Metacyclops mendocinus) from Patagonia, Argentina

  • Paper
  • Published:
Photochemical & Photobiological Sciences Aims and scope Submit manuscript

    We’re sorry, something doesn't seem to be working properly.

    Please try refreshing the page. If that doesn't work, please contact support so we can address the problem.

Abstract

The impact of ultraviolet-B radiation (UV-B, 280–315 nm) on the cladoceran Daphnia menucoensis Paggi and the copepod Metacyclops mendocinus (Wierzejski) was determined in experiments designed to evaluate the effectiveness of the photorepair mechanism and the role of UV-absorbing compounds. In both species UV-B caused significant mortality at doses of ∼40 kJ m−2 or higher. At lower UV-B doses, however, no significant mortality was detected in M. mendocinus; moreover, this species seems to have a threshold below which no UV-B induced mortality is determined. D. menucoensis, on the other hand, was very sensitive to UV-B, and significant mortality of 15% (p < 0.05) was observed when doses were as low as 10 kJ m−2. Both species showed high efficiency for photorepairing UV-B-induced damage to the DNA molecule, with a significant decrease of mortality when the species were exposed to visible radiation, PAR (55 W m−2), in addition to UV-B. The higher resistance of M. mendocinus to UV-B as compared to that of D. menucoensis might be also related to the presence of mycosporine-like amino acids, MAAs (i.e., shinorine and porphyra-334), and carotenoids, which would add an adaptive advantage to the copepod.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. J. C. Farman, B. G. Gardiner, J. D. Shanklin, Large losses of total ozone in Antarctica reveal seasonal ClOx/NOx interaction, Nature, 1985, 315, 207–210.

    Article  CAS  Google Scholar 

  2. A. R. Young, L. O. Björn, J. Moan and W. Nultsch, Environmental UV Photobiology, Plenum Press, New York, 1993, p. 479.

    Book  Google Scholar 

  3. S. J. De Mora, S. Demers and M. Vernet, The effects of UV radiation on marine ecosystems, Cambridge University Press, Cambridge, 2000, p. 324.

    Book  Google Scholar 

  4. E. W. Helbling and H. E. Zagarese, UV effects in aquatic organisms and ecosystems, Comprehensive Series in Photochemical and Photobiological Sciences, The Royal Society of Chemistry, Cambridge, 2002, in press.

    Google Scholar 

  5. H. E. Zagarese and C. E. Williamson, Impact of solar UV radiation on zooplankton and fish, in The effects of UV radiation in the marine environment, eds. S. De Mora, S. Demers and M. Vernet, Cambridge Environmental Chemistry Series 10, Cambridge University Press, Cambridge, 2000, pp. 279–309.

  6. D. O. Hessen, UVR and pelagic metazoans, in UV effects in aquatic organisms and ecosystems, eds. E. W. Helbling and H. E. Zagarese, Comprehensive Series in Photochemical and Photobiological Sciences, The Royal Society of Chemistry, Cambridge, 2002, in press.

  7. J. H. M. Kowenberg, H. I. Browman, J. A. Runge, J. J. Cullen, R. F. Davis, J. F. St-Pierre, Biological weighting of ultraviolet (280–400 nm) induced mortality in marine zooplankton and fish. II Calanus finmarchicus (Copepoda) eggs, Mar. Biol., 1999, 134, 285–293.

    Article  Google Scholar 

  8. C. Alonso Rodriguez, H. I. Browman, J. A. Runge, J.-F. St-Pierre, Impact of solar ultraviolet radiation on hatching of a marine copepod Calanus finmarchicus, Mar. Ecol. Prog. Ser., 2000, 193, 85–93.

    Article  Google Scholar 

  9. E. W. Helbling, F. Zaratti, L. O. Sala, E. R. Palenque, C. F. Menchi, V. E. Villafañe, Mycosporine like aminoacids protect the copepod Boeckella titicacae against high levels of solar UVR, J. Plankton Res., 2002, 24, 225–234.

    Article  CAS  Google Scholar 

  10. K. A. Aarseth, T. A. Schram, Wavelength-specific behaviour in Lepeophthreirus salmonis and Calanus finmarchicus to ultraviolet and visible light in laboratory experiments (Crustacea: Copepoda), Mar. Ecol. Prog. Ser., 1999, 186, 211–217.

    Article  Google Scholar 

  11. D. M. Leech, C. E. Williamson, In situ exposure to ultraviolet radiation alters the depth distribution of Daphnia, Limnol. Oceanogr., 2001, 46, 416–420.

    Article  Google Scholar 

  12. S. C. Rhode, M. Pawlowski, R. Tollrian, The impact of ultraviolet radiation on the vertical distribution of zooplankton of the genus Daphnia, Nature, 2001, 412, 69–72.

    Article  CAS  Google Scholar 

  13. K. D. Malloy, M. A. Holman, D. Mitchell, H. W. I. Dietrich, Solar UVB-induced DNA damage and photoenzymatic DNA repair in Antarctic zooplankton, Proc. Natl. Acad. Sci. USA, 1997, 94, 1258–1263.

    Article  CAS  Google Scholar 

  14. D. L. Mitchell and D. Karentz, The induction and repair of DNA photodamage in the environment, in Environmental UV Photobiology, eds. A. R. Young, L. O. Björn, J. Moan and W. Nultsch, Plenum Press, New York, 1993, pp. 345–377.

  15. A. T. Banaszak, Photoprotective physiological and biochemical responses by marine and freshwater organisms to UVR, in UV effects in aquatic organisms and ecosystems, eds. E. W. Helbling and H. E. Zagarese, Comprehensive Series in Photochemical and Photobiological Sciences, The Royal Society of Chemistry, Cambridge, 2002, in press.

  16. H. E. Zagarese, M. Feldman, C. E. Williamson, UV-B induced damage and photoreactivation in three species of Boeckella (Copepoda, Calanoida), J. Plankton Res., 1997, 19, 357–367.

    Article  Google Scholar 

  17. T. Naganuma, T. Inoue, S. Uye, Photoreactivation of UV-induced damage to embryos of a planktonic copepod, J. Plankton Res., 1997, 19, 783–787.

    Article  Google Scholar 

  18. H. E. Zagarese, C. E. Williamson, T. L. Vail, O. Olsen, C. Queimaliños, Long-term exposure of Boeckella gibbosa (Copepoda, Calanoida) to in situ levels of solar UVB radiation, Freshwater Biol., 1997, 37, 99–106.

    Article  Google Scholar 

  19. H. E. Zagarese, W. R. Cravero, P. González, F. Pedrozo, Copepod mortality induced by fluctuating levels of natural ultraviolet radiation simulating vertical water mixing, Limnol. Oceanogr., 1998, 43, 169–174.

    Article  CAS  Google Scholar 

  20. H. E. Zagarese, B. Tartarotti, W. R. Cravero, P. González, UV damage in shallow lakes: The implications of water mixing, J. Plankton Res., 1998b, 20, 1423–1433.

    Article  Google Scholar 

  21. R. P. Sinha, M. Klisch, D.-P. Häder, Induction of a mycosporine-like amino acid (MAA) in the rice-field cyanobacterium Anabaena sp. by UV irradiation, J. Photochem. Photobiol. B: Biol., 1999, 52, 59–64.

    Article  CAS  Google Scholar 

  22. S. J. Newman, S. Nicol, D. Ritz, H. J. Marchant, Susceptibility of Antarctic krill (Euphausia superba Dana) to ultraviolet radiation, Polar Biol., 1999, 22, 50–55.

    Article  Google Scholar 

  23. V. E. Rocco, O. Oppezzo, R. Pizarro, R. Sommaruga, M. Ferraro, H. E. Zagarese, Ultraviolet damage and counteracting mechanisms in the freshwater copepod Boeckella poppei from the Antarctic Peninsula, Limnol. Oceanogr., 2002, 47, 829–836.

    Article  Google Scholar 

  24. O. Siebeck, U. Böhm, Challenges for an appraisal of UV-B effects upon planktonic crustaceans under natural radiation conditions with a non-migrating (Daphnia pulex obtusa) and a migrating cladoceran (Daphnia galeata), Arch. Hydrobiol. Beih. Ergeb. Limnol., 1994, 43, 197–206.

    Google Scholar 

  25. G. Grad, C. E. Williamson, D. M. Karapelou, Zooplankton survival and reproduction responses to damaging UV radiation: A test of reciprocity and photoenzymatic repair, Limnol. Oceanogr., 2001, 46, 584–591.

    Article  Google Scholar 

  26. A. Cywinska, D. Crump, D. Lean, Influence of UV radiation on four freshwater invertebrates, Photochem. Photobiol., 2000, 72, 652–659.

    Article  CAS  Google Scholar 

  27. B. Tartarotti, I. Laurion, R. Sommaruga, Large variability in the concentration of mycosporine-like amino acids among zooplankton from lakes located across an altitude gradient, Limnol. Oceanogr., 2001, 46, 1546–1552.

    Article  CAS  Google Scholar 

  28. N. G. Hairston, The adaptive significance of color polymorphism in two species of Diaptomus (Copepoda), Limnol. Oceanogr., 1979, 24, 15–37.

    Article  CAS  Google Scholar 

  29. D. O. Hessen, K. Sørensen, Photoprotective pigmentation in alpine zooplankton populations, Aqua Fenn., 1990, 20, 165–170.

    CAS  Google Scholar 

  30. D. O. Hessen, Daphnia responses to UV-B light, Arch. Hydrobiol. Beih. Ergeb. Limnol., 1994, 43, 185–195.

    Google Scholar 

  31. S. Madronich, The atmosphere and UV-B radiation at ground level, in Environmental UV Photobiology, eds. A. R. Young, L. O. Björn, J. Moan and W. Nultsch, Plenum Press, New York, 1993, pp. 1–39.

  32. V. E. Villafañe, E. W. Helbling, H. E. Zagarese, Solar ultraviolet radiation and its impact on aquatic systems of Patagonia, South America, Ambio, 2001, 30, 104–111.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Estación de Fotobiología Playa Unión, Casilla de Correo 153, 9100, Trelew, Chubut, Argentina

    Rodrigo J. Gonçalves, Virginia E. Villafañe & E. Walter Helbling

  2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina

    Virginia E. Villafañe & E. Walter Helbling

Authors
  1. Rodrigo J. Gonçalves
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Virginia E. Villafañe
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Walter Helbling
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gonçalves, R.J., Villafañe, V.E. & Helbling, E.W. Photorepair activity and protective compounds in two freshwater zooplankton species (Daphnia menucoensis and Metacyclops mendocinus) from Patagonia, Argentina. Photochem Photobiol Sci 1, 996–1000 (2002). https://doi.org/10.1039/b208145h

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1039/b208145h

Search

Navigation