Abstract
Charge recombination kinetics of bacterial photosynthetic protein Reaction Center displays an exquisite sensitivity to the actual occupancy of ubiquinone-10 in its QB-binding site. Here, we have exploited such phenomenon for assessing the growth and the aggregation/fusion of phosphocholine vesicles embedding RC in their membrane, when treated with sodium oleate.
Notes
Aggregation and fusion can happen because of the propensity of oleic acid molecules, formed by OL protonation, to self-assemble as non-lamellar inverted hexagonal structures, which can be fusogenic. At even lower pH, oleic acid separates as oil droplets [30].
In principle, in addition to fusion and aggregation, RC and/or Q could be shuttled between different vesicles by hydrophobic oleic acid/oleate-based carriers.
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Acknowledgements
The authors are grateful to Massimo Trotta (Institute for Physical and Chemical Processes, CNR-Bari, Italy) for helpful discussions.
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Electronic Supplementary Information (ESI) available: RC charge recombinationmechanism; liposome preparation and composition; S value calculation; kineticspectrometer description. See DOI: https://doi.org/10.1039/x0xx00000x (DOCX 178 KB)
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Altamura, E., Milano, F., Stano, P. et al. Exploiting the photoactivity of bacterial reaction center to investigate liposome dynamics. Photochem Photobiol Sci 20, 321–326 (2021). https://doi.org/10.1007/s43630-021-00011-3
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DOI: https://doi.org/10.1007/s43630-021-00011-3