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Effects of Ultraviolet B Radiation on the Function of the Testicles, Expression of Caspase-3 and NOS-2, and the Protective Role of Naringin in Mice

  • Reproductive Endocrinology: Original Article
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Abstract

In today’s evolving global environment, reproductive dysfunctions brought on by various environmental toxins are of greatest concern. Radiation is a constant threat to living things, causing both genetic and cellular changes that result in mutations and cell death. It is thought that ultraviolet B (UVB) radiation we are exposed to daily has biological effects on rats and humans that are both short and long term. Due to the damaging effects of UVB radiation on the living system, this study explores the automatic mechanism by which a certain level of radiation induces oxidative stress, which is further controlled by the antioxidant activity of naringin (NG). In our study, male Swiss albino mice were exposed to UVB irradiation, which altered mice’s body and testes weight, hormonal imbalance, biochemical parameters, and histo-morphometric parameter. In addition, we chose naringin’s UVB irradiation deterrent effect. Twenty-four healthy adult male Swiss albino mice weighing 25–35 g were chosen at random. For 15 days of exposure, they were divided into four groups at random: group I—control, group II—UVB exposure (2 h per day), group III—UVB exposure with naringin (NG) (80 mg/kg, bw), and group IV—naringin (NG) (80 mg/kg, bw). Compared to the control group, UVB irradiation causes alterations in the animal body weight, testes weight, hormones, enzymatic and non-enzymatic assays, and histological parameters. It was seen that NG retrieved the alterations in parameters caused by UVB irradiation. The UVB radiation exposure on mice caused the testicular dysfunction drastically, while the naringin recapitulates testis functioning.

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Acknowledgements

The authors acknowledge the DST-FIST-sponsored Department of Zoology, School of Biological Sciences, Dr. Harisingh Gour Vishwavidyalaya, Sagar, M.P., India, for providing infrastructure facilities.

Funding

This research work was financially supported by UGC NON-NET FELLOWSHIP which boosted this research work efficaciously.

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  1. Endocrinology Laboratory, Department of Zoology, Dr. Harisingh Gour Vishwavidyalaya, Sagar, Madhya Pradesh, India

    Shashank Shakyawal, Narendra Namdev & Payal Mahobiya

  2. Cancer Biology Laboratory, Department of Zoology, Dr. Harisingh Gour Vishwavidyalaya, Sagar, Madhya Pradesh, India

    Zaved Ahmad

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  1. Shashank Shakyawal
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PM designed the experiment plan. SS managed the experimental animals, performed the treatment, completed the data analysis, and wrote the manuscript. NN and ZA contributed to the editing and completion of the manuscript. All the authors read and approved the final manuscript.

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Correspondence to Payal Mahobiya.

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Shakyawal, S., Namdev, N., Ahmad, Z. et al. Effects of Ultraviolet B Radiation on the Function of the Testicles, Expression of Caspase-3 and NOS-2, and the Protective Role of Naringin in Mice. Reprod. Sci. 31, 452–468 (2024). https://doi.org/10.1007/s43032-023-01366-x

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