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Cross-Inoculation of Elite Commercial Bradyrhizobium Strains from Cowpea and Soybean in Mung Bean and Comparison with Mung Bean Isolates

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Abstract

The objective of this study was to evaluate the efficiency of nodulation and N2 fixation of strains Bradyrhizobium recommended for cowpea and soybean when used as inoculants for mung bean in comparison with Bradyrhizobium isolates obtained from mung bean nodules. This study will contribute to the process of recommending an inoculant for mung bean and determine if any existing commercial inoculant is efficient in nodulation of this crop. An experiment was conducted in a greenhouse under axenic conditions for the cross-inoculation of eight elite strains used in commercial inoculants. Subsequently, the efficiency of these strains was examined in a pot experiment with non-sterile soil and compared with that of the 13 Bradyrhizobium isolates. Results revealed that the SEMIA 587 (Bradyrhizobium elkanii) strain used as commercial inoculants for soybean and the UFLA 3-84 (B. viridifuturi), BR 3267 (B. yuanmingense), and INPA 03-11B (B. elkanii) strains used for cowpea could efficiently nodulate mung bean. Conversely, BR 3262 (B. pachyrhizi) for cowpea and CPAC 15 (B. japonicum), CPAC 7 (B. diazoefficiens), and BR 29 (29 W; B. elkanii) for soybean could not achieve the same outcome. Although mung bean is considered a promiscuous legume, these results suggest the existence of symbiotic incompatibility with some Bradyrhizobium strains. The efficiency of the elite SEMIA 587 (B. elkanii) and UFLA 3–84 (B. viridifuturi) strains in terms of increased nodulation and plant growth was similar to those of Bradyrhizobium strains isolated from mung bean nodules and, therefore, should be evaluated under field conditions to verify their contribution to biological nitrogen fixation in mung bean.

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Funding

This work was supported by the Carlos Chagas Filho Foundation for the Support of Research in the State of Rio de Janeiro (FAPERJ) [Project: E-26/202.546/2019], the Coordination for the Improvement of Higher Education Personnel (CAPES)-Financing Code 001, and the Brazilian National Council for Scientific and Technological Development (CNPq).

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  1. Instituto de Agronomia, Universidade Federal Rural do Rio de Janeiro, BR 465, km 07, Seropédica, Rio de Janeiro, 23890-000, Brazil

    Vinício Oliosi Favero, Rita Hilário de Carvalho, Ana Beatriz Carneiro Leite & Dieini Melissa Teles dos Santos

  2. Embrapa Agrobiologia, BR 465, km 07, Seropédica, Rio de Janeiro, 23891-000, Brazil

    Karine Moura de Freitas, Jerri Édson Zilli, Gustavo Ribeiro Xavier, Norma Gouvêa Rumjanek & Segundo Urquiaga

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  1. Vinício Oliosi Favero
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  2. Rita Hilário de Carvalho
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  3. Ana Beatriz Carneiro Leite
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Favero, V.O., de Carvalho, R.H., Leite, A.B.C. et al. Cross-Inoculation of Elite Commercial Bradyrhizobium Strains from Cowpea and Soybean in Mung Bean and Comparison with Mung Bean Isolates. J Soil Sci Plant Nutr 22, 4356–4364 (2022). https://doi.org/10.1007/s42729-022-01034-0

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