Abstract
The application of urea fertilizer on soil surfaces causes hydrolysis reactions to occur rapidly, thus releasing ammonia gas to the atmosphere and, in turn, reducing the availability of nitrogen for plant uptake. The urease inhibitor application with urea is considered a mitigation method to reduce the loss of nitrogen. Chemical-based urease inhibitors effectively reduce nitrogen loss, but they also demonstrate adverse side effects on plants. Thus, there is a need to discover potential candidates for urease inhibitors from natural sources. This study evaluates the potential of organosulfur compounds from garlic, namely, allicin, diallyl sulfide (DAS), and diallyl disulfide (DADS), as inhibitors to urea hydrolysis. The effect of allicin, DAS, and DADS on kinetic parameters of soil urease and urea hydrolysis rate was evaluated using the Lineweaver–Burk plot and incubation study. All tested organosulfur compounds exhibited mixed-type inhibition with a high Michaelis constant (Km) and low maximum velocity of reaction (Vmax), compared with control (urea). The calculated inhibitory (dissociation) constant (Ki) for allicin, DAS, and DADS were 0.53, 0.92, and 0.40 mM, respectively. This indicates that DADS is the most potent soil urease inhibitor, followed by allicin and DAS. After DADS treatment, about 46% of the urea-nitrogen (N) content remained after 21 days. Meanwhile, allicin and DAS treatments retained urea-N in the soil for 7 and 11 days, respectively. The results demonstrate that DADS is more effective in delaying enzymatic urea hydrolysis compared with allicin and DAS and showed a high potential as a bio-based urease inhibitor in agriculture.
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Data are available on request from the authors.
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Acknowledgements
The authors would like to offer their utmost appreciation to Neem Biotech Ltd for providing the allicin. The authors also thank Universiti Teknologi PETRONAS for supporting the work by providing a graduate assistantship.
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Salehuddin, N.F., Mansor, N., Yahya, W.Z.N. et al. Organosulfur Compounds as Soil Urease Inhibitors and Their Effect on Kinetics of Urea Hydrolysis. J Soil Sci Plant Nutr 21, 2652–2659 (2021). https://doi.org/10.1007/s42729-021-00553-6
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DOI: https://doi.org/10.1007/s42729-021-00553-6