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Assessment of Nitrogen Fixation by Mungbean Genotypes in Different Soil Textures Using 15N Natural Abundance Method

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Abstract

Ensuring food and nutritional security in light of high climate variability and a rapidly growing population remains a challenge. Mungbean (Vigna radiata (L.) Wilczek) is a short duration, drought-tolerant, and ureide-exporting legume crop capable of symbiotic atmospheric nitrogen fixation. Estimates of biological N2 fixation by mungbean in different soil textures have not been extensively studied. We conducted this study to evaluate plant growth and N2 fixation of five mungbean genotypes (Berken, 8735, IC 8972-1, STB#122, 223) inoculated with Bradyrhizobium spp. and grown on loamy sand and silt loam soils under glasshouse conditions. Mungbean dry matter yield, δ15N values, shoot content, amounts of N-fixed, and soil N uptake were all higher on the silt loam soil compared to the loamy sand soil, demonstrating the effects of soil properties on plant growth and N2 fixation potential. Among genotypes, IC 8972-1 produced the highest biomass (7.85 g plant−1), shoot N content (200 mg plant−1), and soil N uptake (155 mg plant−1) than other genotypes. The significant interaction between soil texture and genotypes for root dry matter and %Ndfa indicates the major role of legume root-nodule bacteria in symbiotic N2 fixation. This study demonstrated that N2 fixation in mungbean is affected by both genotypes and soil properties, illustrating the need to consider soil properties in order to maximize N contribution from mungbean to agricultural production systems.

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References

  • Ahmad S, Ali Khan A, Ali S, Ullah I, Imran M, Habibullah M (2015) Impact of phoshporus levels on yield and yield attributes of mungbean cultivars under peshawar valley conditions vol 5

  • Ali H, Khan E, Sajad MA (2013a) Phytoremediation of heavy metals-concepts and applications. Chemosphere 91:869–881

    Article  CAS  Google Scholar 

  • Ali S, Hasan A, Ijaz S, Ansar M (2013b) Mungbean (Vigna radiata) yield and di-nitrogen fixation under minimum tillage at semi arid Pothwar. Pakistan J Anim Plant Sci 23:198–202

    CAS  Google Scholar 

  • Ayisi KK, Nkgapele RJ, Dakora FD (2000) Nodule formation and function in six varieties of cowpea (Vigna unguiculata L. Walp.) grown in a nitrogen-rich field soil in South Africa Symbiosis. https://doi.org/10.1016/j.soilbio.2012.05.017

  • Belane AK, Dakora FD (2010) Symbiotic N 2 fixation in 30 field-grown cowpea (Vigna unguiculata L. Walp.) genotypes in the upper west region of Ghana measured using 15 N natural abundance. Biol Fertil Soils 46:191–198. https://doi.org/10.1007/s00374-009-0415-6

    Article  Google Scholar 

  • Beyan SM, Wolde-meskel E, Dakora FD (2018) An assessment of plant growth and N 2 fixation in soybean genotypes grown in uninoculated soils collected from different locations in Ethiopia Symbiosis:1-15. https://doi.org/10.1007/s13199-018-0540-9

  • Bremner JM, Keeney DR (1966) Determination and isotope-ratio analysis of different forms of nitrogen in soils: 3. Exchangeable ammonium, nitrate, and nitrite by extraction-distillation methods Proceedings Soil Science Society of America 30:577–582 https://doi.org/10.2136/sssaj1966.03615995003000050015x

  • Chadha M (2001) Mungbean (Vigna radiata L.), a choice crop for improvement of human and soil health in southern Africa. In: combating desertification with plants. Springer, pp 263-271. https://doi.org/10.1007/978-1-4615-1327-8_25

  • Chauhan Y, Williams R (2018) Physiological and agronomic strategies to increase mungbean yield in climatically variable environments of Northern Australia Agronomy 8:83 https://doi.org/10.3390/agronomy8060083

  • Crews T, Brockwell J, Peoples M (2004) Host–rhizobia interaction for effective inoculation: evaluation of the potential use of the ureide assay to monitor the symbiotic performance of tepary bean (Phaseolus acutifolius a. gray). Soil Biol Biochem 36:1223–1228. https://doi.org/10.1016/j.soilbio.2003.09.016

    Article  CAS  Google Scholar 

  • Delfin EF, Paterno ES, Torres° FG, Santos PJA (2008) Biomass, nitrogen uptake and fixed nitrogen partitioningin field grown mungbean (Vigna radiata L. Wilczek) inoculated with Bradyrhizobium. Philipp J Crop Sci (PJCS) 33:24–33

  • Diatta AA et al (2019) Effect of intercropping mungbean on millet yield in the Peanut basin. Senegal Innovations Agronomiques 74:69–81

    Google Scholar 

  • Diatta AA et al (2016a) Ecosystem services of Cordyla pinnata (Lepr. Ex a. rich.) Milne-Redh. Agroforestry parkland of Senegal’s South Peanut Basin. Int J Biol Chem Sci 10:2511–2525. https://doi.org/10.4314/ijbcs.v10i6.9

    Article  Google Scholar 

  • Diatta AA, Ndour N, Manga A, Sambou B, Faye CS, Diatta L, Mbow C (2016b) Floristic composition and dynamics of Cordyla pinnata (Lepr. Ex a. rich.) Milne-Redh. Agroforestry parkland of Senegal’s South Peanut Basin. Int J Biol Chem Sci 10:1805–1822. https://doi.org/10.4314/ijbcs.v10i4.29

    Article  Google Scholar 

  • Diatta AA et al (2018) Inoculation and soil texture effects on yield and yield components of mungbean. J Agric Sci (Toronto) 10:6–16. https://doi.org/10.5539/jas.v10n9p6

    Article  Google Scholar 

  • Dudeja S, Duhan J (2005) Biological nitrogen fixation research in pulses with special reference to mungbean and urdbean. Indian J Pulses Res 18:107

    Google Scholar 

  • Foyer CH, Lam HM, Nguyen HT, Siddique KHM, Varshney RK, Colmer TD, Cowling W, Bramley H, Mori TA, Hodgson JM, Cooper JW, Miller AJ, Kunert K, Vorster J, Cullis C, Ozga JA, Wahlqvist ML, Liang Y, Shou H, Shi K, Yu J, Fodor N, Kaiser BN, Wong FL, Valliyodan B, Considine MJ (2016) Neglecting legumes has compromised human health and sustainable food production. Nature Plants 2:16112. https://doi.org/10.1038/nplants.2016.112

    Article  PubMed  Google Scholar 

  • Franke A, Van den Brand G, Vanlauwe B, Giller K (2018) Sustainable intensification through rotations with grain legumes in sub-Saharan Africa: a review agriculture, ecosystems & environment 261:172-185 https://doi.org/10.1016/j.agee.2017.09.029

  • George T, Ladha JK, Garrity DP, Torres RO (1995) Nitrogen dynamics of grain legume-weedy fallow-flooded rice sequences in the tropics. Agron J 87:1–6. https://doi.org/10.2134/agronj1995.00021962008700010001x

    Article  Google Scholar 

  • Giller KE (2001) Nitrogen fixation in tropical cropping systems. Cabi. https://doi.org/10.1079/9780851994178.0000

  • Graham PH, Vance CP (2003) Legumes: importance and constraints to greater use. Plant Physiol 131:872–877. https://doi.org/10.1104/pp.017004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Gupta SC, Larson WE (1979) Estimating soil water retention characteristics from particle size distribution, organic matter percent, and bulk density. Water Resour Res 15:1633–1635. https://doi.org/10.1029/WR015i006p01633

    Article  Google Scholar 

  • Haider MU, Hussain M, Farooq M, Nawaz A (2020) Zinc nutrition for improving the productivity and grain biofortification of Mungbean journal of soil science and plant nutrition:1-15 https://doi.org/10.1007/s42729-020-00215-z

  • Hayat R, Ali S (2010) Nitrogen fixation of legumes and yield of wheat under legumes-wheat rotation in Pothwar Pak J Bot 42:2317-2326

  • Hayat R, Ali S, Ijaz SS, Chatha TH, Siddique MT (2008) Estimation of N2-fixation of mung bean and mash bean through xylem uriede technique under rainfed conditions. Pak J Bot 40:723–734

    CAS  Google Scholar 

  • Herridge D, Robertson M, Cocks B, Peoples M, Holland J, Heuke L (2005) Low nodulation and nitrogen fixation of mungbean reduce biomass and grain yields. Aust J Exp Agric 45:269–277. https://doi.org/10.1071/ea03130

    Article  CAS  Google Scholar 

  • Herridge D, Rose I (2000) Breeding for enhanced nitrogen fixation in crop legumes. Field Crop Res 65:229–248. https://doi.org/10.1007/978-94-009-5077-1

    Article  Google Scholar 

  • Herridge DF, Peoples MB (2002) Calibrating the xylem-solute method for nitrogen fixation measurement of ureide-producing legumes: cowpea, mungbean, and black gram Communications in soil science and plant analysis 33:425-437 https://doi.org/10.1081/css-120002754

  • Herridge DF, Peoples MB, Boddey RM (2008) Global inputs of biological nitrogen fixation in agricultural systems. Plant Soil 311:1–18. https://doi.org/10.1007/s11104-008-9668-3

    Article  CAS  Google Scholar 

  • Keatinge J, Easdown W, Yang R, Chadha M, Shanmugasundaram S (2011) Overcoming chronic malnutrition in a future warming world: the key importance of mungbean and vegetable soybean. Euphytica 180:129–141. https://doi.org/10.1007/s10681-011-0401-6

    Article  Google Scholar 

  • Kermah M, Franke A, Adjei-Nsiah S, Ahiabor B, Abaidoo RC, Giller K (2018) N2-fixation and N contribution by grain legumes under different soil fertility status and cropping systems in the Guinea savanna of northern Ghana agriculture, ecosystems & environment 261:201-210 https://doi.org/10.1016/j.agee.2017.08.028

  • Khan A, Malik MA (2001) Determining biological yield potential of different mungbean cultivars. On line J Biol Sci 1:575–576. https://doi.org/10.3923/jbs.2001.575.576

    Article  Google Scholar 

  • Maguire R, Heckendorn SE (2011) Laboratory procedures: Virginia Tech soil testing laboratory. Virginia Cooperative Extension, Blacksburg, VA. Available at http://pubs.ext.vt.edu/452/452-881/452-881_pdf.pdf

  • Mapope N, Dakora FD (2016) N2 fixation, carbon accumulation, and plant water relations in soybean (Glycine max L. Merrill) varieties sampled from farmers’ fields in South Africa, measured using 15N and 13C natural abundance agriculture. Ecosyst Environ 221:174–186. https://doi.org/10.1016/j.agee.2016.01.023

    Article  CAS  Google Scholar 

  • Maseko ST, Maredi MP, Mathews C, Dakora FD (2020) Harnessing ecosystem services from biological nitrogen fixation. In: The Role of Ecosystem Services in Sustainable Food Systems. Elsevier, pp 73–94. https://doi.org/10.1016/b978-0-12-816436-5.00004-4

  • Matusso J, Mugwe J, Mucheru-Muna M (2014) Potential role of cereal-legume intercropping systems in integrated soil fertility management in smallholder farming systems of sub-Saharan Africa Journal of Agriculture and Environmental Management 3:162–174

  • Mohammad W, Shehzadi S, Shah SM, Shah Z (2010) Effect of tillage and crop residues management on mungbean (Vigna radiata (L.) Wilczek) crop yield, nitrogen fixation and water use efficiency in rain fed areas. Pak J Bot 42:1781–1789

    Google Scholar 

  • Mokgehle SN, Dakora FD, Mathews C (2014) Variation in N2 fixation and N contribution by 25 groundnut (Arachis hypogaea L.) varieties grown in different agro-ecologies, measured using 15N natural abundance. Agric Ecosyst Environ 195:161–172. https://doi.org/10.1016/j.agee.2014.05.014

    Article  CAS  Google Scholar 

  • Mondal M, Puteh A, Malek M, Ismail M, Rafii M, Latif M (2012) Seed yield of mungbean (Vigna radiata (L.) Wilczek) in relation to growth and developmental aspects Sci World J 2012 https://doi.org/10.1100/2012/425168

  • Nair RM, Pandey AK, War AR, Hanumantharao B, Shwe T, Alam AKMM, Pratap A, Malik SR, Karimi R, Mbeyagala EK, Douglas CA, Rane J, Schafleitner R (2019) Biotic and abiotic constraints in mungbean production—Progress in genetic improvement. Front Plant Sci 10:10. https://doi.org/10.3389/fpls.2019.01340

    Article  Google Scholar 

  • NRCS (2019) Natural resource conservation service. Web Soil Survey. Available online: http://websoilsurvey.nrcs.usda.gov/app/HomePage.htm (accessed on 24 February 2020)

  • Ntukamazina N, Onwonga RN, Sommer R, Mukankusi CM, Mburu J, Rubyogo JC (2017) Effect of excessive and minimal soil moisture stress on agronomic performance of bush and climbing bean (Phaseolus vulgaris L.). Cogent Food Agric 3:1373414. https://doi.org/10.1080/23311932.2017.1373414

    Article  CAS  Google Scholar 

  • Pandey AK, Burlakoti RR, Kenyon L, Nair RM (2018) Perspectives and challenges for sustainable management of fungal diseases of mungbean [Vigna radiata (L.) R. Wilczek var. radiata]: a review Frontiers in environmental science 6:53 https://doi.org/10.3389/fenvs.2018.00053

  • Peoples M, Bergersen F, Turner G (1991) Use of the natural enrichment of 15 N in plant available soil N for the measurement of symbiotic N 2 fixation. In: Stable isotopes in plant nutrition, soil fertility and environmental studies

  • Peoples M et al (2009) The contributions of nitrogen-fixing crop legumes to the productivity of agricultural systems. Symbiosis 48:1–17. https://doi.org/10.1007/bf03179980

    Article  CAS  Google Scholar 

  • Peoples M, Herridge D, Ladha J (1995) Biological nitrogen fixation: an efficient source of nitrogen for sustainable agricultural production? In: Management of Biological Nitrogen Fixation for the Development of More Productive and Sustainable Agricultural Systems. Springer, pp 3–28. https://doi.org/10.1007/978-94-011-0055-7_1

  • Rahim M, Mia A, Mahmud F, Zeba N, Afrin K (2010) Genetic variability, character association and genetic divergence in mungbean ('Vigna radiata'L. Wilczek). Plant Omics 3:1. https://doi.org/10.18805/ijare.a-4929

    Article  Google Scholar 

  • Raji SG, Tzanakakis V, Dörsch P (2019) Bradyrhizobial inoculation and P application effects on haricot and mung beans in the Ethiopian Rift Valley Plant and Soil 442:271–284 https://doi.org/10.1007/s11104-019-04170-2

  • Rao C, Ali M (2006) Response and nutrient uptake of urdbean and mungbean genotypes to optimum nutrient supply on nutrient deficient sandy loam soil. Indian J Pulses Res 19:259–262

    Google Scholar 

  • Reeves T, Thomas G, Ramsay G (2016) Save and grow in practice: maize, rice, wheat--a guide to sustainable cereal production UN food and agriculture organization. Rome 8:1207–1208. https://doi.org/10.1007/s12571-016-0585-7

    Article  Google Scholar 

  • Robinson D (2001) δ15N as an integrator of the nitrogen cycle trends in Ecology & Evolution 16:153-162. https://doi.org/10.1016/s0169-5347(00)02098-x

  • Romanyà J, Casals P (2019) Biological nitrogen fixation response to soil fertility is species-dependent in annual legumes J Soil Sci Plant Nutr:1-11 https://doi.org/10.1007/s42729-019-00144-6

  • Rosales C, Rivera F, Hautia R, Del Rosario E (1995) Nitrogen fixation by mung bean (Vigna radiata L.) under field conditions in the Philippines as quantified by 15N isotope dilution. Philippine nuclear research Inst

  • Shah Z, Shah SH, Peoples MB, Schwenke GD, Herridge DF (2003) Crop residue and fertiliser N effects on nitrogen fixation and yields of legume–cereal rotations and soil organic fertility. Field Crop Res 83:1–11. https://doi.org/10.1016/S0378-4290(03)00005-4

    Article  Google Scholar 

  • Shearer G, Kohl DH (1986) N2-fixation in field settings: estimations based on natural 15N abundance. Funct Plant Biol 13:699–756. https://doi.org/10.1071/pp9860699

    Article  CAS  Google Scholar 

  • Singh D, Mathimaran N, Boller T, Kahmen A (2020) Deep-rooted pigeon pea promotes the water relations and survival of shallow-rooted finger millet during drought—Despite strong competitive interactions at ambient water availability. PLoS One 15:e0228993. https://doi.org/10.1371/journal.pone.0228993

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Singh G, Sekhon H, Singh G, Brar J, Bains T, Shanmugasundaram S (2011) Effect of plant density on the growth and yield of mungbean [Vigna radiata (L.) Wilczek] genotypes under different environments in India and Taiwan. Int J Agric Res 6:573–583. https://doi.org/10.3923/ijar.2011.573.583

    Article  Google Scholar 

  • Tharanathan R, Mahadevamma S (2003) Grain legumes - a boon to human nutrition Trends in Food Science & Technology 14:507–518 https://doi.org/10.1016/j.tifs.2003.07.002

  • Thilakarathna MS, McElroy MS, Chapagain T, Papadopoulos YA, Raizada MN (2016) Belowground nitrogen transfer from legumes to non-legumes under managed herbaceous cropping systems. Agron Sustain Dev 36:58. https://doi.org/10.1007/s13593-016-0396-4

    Article  CAS  Google Scholar 

  • Thomson B, Siddique K, Barr M, Wilson J (1997) Grain legume species in low rainfall Mediterranean-type environments I. Phenology and seed yield Field Crops Research 54:173–187 https://doi.org/10.1016/s0378-4290(97)00047-6

  • Travlos I, Karamanos A (2006) Effects of soil texture on vegetative growth of tropical legume marama bean (Tylosema esculentum). J Agron 5:609–612. https://doi.org/10.3923/ja.2006.609.612

    Article  Google Scholar 

  • Unkovich M et al. (2008) Measuring plant-associated nitrogen fixation in agricultural systems. Australian Centre for International Agricultural Research (ACIAR),

  • Unkovich MJ, Pate JS, Sanford P, Armstrong EL (1994) Potential precision of the δ15N natural abundance method in field estimates of nitrogen fixation by crop and pasture legumes in south-West Australia Australian journal of agricultural research 45:119-132 https://doi.org/10.1007/s11104-012-1330-4

  • Vanlauwe B, Hungria M, Kanampiu F, Giller K (2019) The role of legumes in the sustainable intensification of African smallholder agriculture: lessons learnt and challenges for the future agriculture. Ecosyst Environ 284:106583. https://doi.org/10.1016/j.agee.2019.106583

    Article  CAS  Google Scholar 

  • Yimram T, Somta P, Srinives P (2009) Genetic variation in cultivated mungbean germplasm and its implication in breeding for high yield. Field Crop Res 112:260–266. https://doi.org/10.1016/j.fcr.2009.03.013

    Article  Google Scholar 

  • Zang H, Yang X, Feng X, Qian X, Hu Y, Ren C, Zeng Z (2015) Rhizodeposition of nitrogen and carbon by mungbean (Vigna radiata L.) and its contribution to intercropped oats (Avena nuda L.). PLoS One 10:e0121132. https://doi.org/10.1371/journal.pone.0121132

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhao C, Jia L, Wang Y, Wang M, McGiffen Jr M (2015) Effects of different soil texture on peanut growth and development Communications in Soil Science and Plant Analysis 46:2249–2257 https://doi.org/10.1080/00103624.2015.1059845

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Acknowledgments

The authors are grateful to the School of Plant and Environmental Sciences (SPES) of Virginia Polytechnic Institute & State University (Virginia Tech) for their support and technical assistance and the Virginia Tech’s Tidewater Agricultural Research and Extension Center (AREC) for providing sorghum seeds.

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  1. School of Plant and Environmental Sciences, Virginia Polytechnic Institute & State University, Blacksburg, VA, 24061, USA

    Andre A. Diatta, Wade E. Thomason, Ozzie Abaye, Thomas L. Thompson, Martin L. Battaglia & Jose F. D. C. L. Filho

  2. Center for International Research, Education and Development, Virginia Polytechnic Institute & State University, Blacksburg, VA, 24061, USA

    Larry J. Vaughan

  3. Institut Sénégalais de Recherches Agricoles, Rte des hydrocarbures, Dakar, Sénégal

    Mamadou Lo

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  1. Andre A. Diatta
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Diatta, A.A., Thomason, W.E., Abaye, O. et al. Assessment of Nitrogen Fixation by Mungbean Genotypes in Different Soil Textures Using 15N Natural Abundance Method. J Soil Sci Plant Nutr 20, 2230–2240 (2020). https://doi.org/10.1007/s42729-020-00290-2

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