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Effects of temperature and pH on the synthesis of nanohydroxyapatite powders by chemical precipitation

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Abstract

Bone tissue engineering is based on a comprehensive understanding of bone structure, bone mechanics, and biology. In order to create nanostructured hydroxyapatite powders with customized properties, many synthesis strategies such as wet chemical precipitation, sol-gel, hydrothermal, and biomimetic approaches have been intensively researched through the years. Calcium phosphate (CaP)-based ceramic nanoparticles, including hydroxyapatite (HAp), were synthesized by the chemical precipitation technique at pH ranges of 7 to 11 and different calcination temperatures of 600 to 1100 °C. The synthesized powders were characterized by several techniques, including scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), energy dispersive X-ray analysis (EDX), and in vitro cell culture assays. The particle size analysis and zeta potential of these powders were also carried out using the dynamic light scattering (DLS) and laser Doppler electrophoresis methods. The results showed that the pH levels of 9 to 11 range and calcination temperatures of 600 to 800 °C were adequate for appropriate nanohydroxyapatite powder production using this method. The particle size of the nanohydroxyapatite was approximately 55 nm, although they were agglomerated after calcination. The biocompatibility tests demonstrated that these nanohydroxyapatite (nHAp) powders produced have appropriate cytocompatibility and can be used for bone graft production and other biomedical applications.

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Data Availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

References

  1. Javaid, M.A., Kaartinen, M.T., Javaid, M.: Mesenchymal stem cell-based bone tissue engineering. International Dental Journal of Students Res. 1, 24–35 (2012)

    Google Scholar 

  2. Kneser, U., Schaefer, D.J., Polykandriotis, E., Horch, R.E.: Tissue engineering of bone: the reconstructive surgeon’s point of view. J. Cell. Mol. Med. 10(1), 7–19 (2006)

  3. Cegla, R.-N.R., Macha, I.J., Ben-Nissan, B., Grossin, D., Heness, G., Chung, R.-J.: Comparative study of conversion of coral with ammonium dihydrogen phosphate and orthophosphoric acid to produce calcium phosphates. J. Aust. Ceram. 50(2), 154–1561 (2014)

    CAS  Google Scholar 

  4. Li, S., Yang, F., Zheng, L., Liu, Y.: Microwave-assisted synthesis of hydroxyapatite nanoparticles: A review. J. Inorganic Mater. 36(2), 141–154 (2021)

    CAS  Google Scholar 

  5. Gao, J., Wang, Z., Li, Y., Liu, Y., Zhu, X.: Recent advances in the synthesis of hydroxyapatite nanomaterials via electrospinning for biomedical applications. J. Biomater. Appl. 36(2), 256–276 (2022)

    Google Scholar 

  6. Ravikumar, N., Zhu, W., Kamruddin, M.: Recent advances in sonochemical synthesis of hydroxyapatite nanoparticles: a comprehensive review. Ultrason. Sonochem. 86, 105480 (2023)

    Google Scholar 

  7. Brown, P.W., Paul, W., Constantz, B.: Hydroxyapatite and related materials. CRC (1994)

    Google Scholar 

  8. Suchanek, W., Yoshimura, M.: Processing and properties of hydroxyapatite-based biomaterials for use as hard tissue replacement implants. J. Mater. Res. 13(1), 94–117 (1998)

  9. Peitl, O., Zanotto, E.D., Serbena, F.C., Hench, L.L.: Bioactive glass-ceramics for load-bearing applications. An Introduction to Bioceramics. 495–503 (2013)

  10. Macha, I.J., Ozyegin, L.S., Chou, J., Samur, R., Oktar, F.N., Ben-Nissan, B.: An Alternative synthesis method for Di calcium phosphate (Monetite) powders from Mediterranean mussel (Mytilus galloprovincialis) Shells. J. Aust. Ceram. Soc. 49(2), 122–128 (2013)

    CAS  Google Scholar 

  11. Vallet-Regí, M., González-Calbet, J.M.: Calcium phosphates as substitution of bone tissues. Prog. Solid State Chem. 32(1–2), 1–31 (2004)

  12. Yang, S., Jin, Y., Sun, H., Xing, Y., Wang, X.: Nanostructured hydroxyapatite-based biomaterials for bone tissue engineering: a review. J. Biomed. Mater. Res. A. 110(1), 15–32 (2022)

    Google Scholar 

  13. Prokopiev, O., Sevostianov, I.: Dependence of the mechanical properties of sintered hydroxyapatite on the sintering temperature. Mater. Sci. Eng. A. 431(1–2), 218–227 (2006)

  14. LeGeros, R.Z.: Calcium phosphates in enamel, dentin and bone. Calcium Phospates in Oral Biology and Medicine. 15, 108–129 (1991)

    Google Scholar 

  15. Boskey, A.L., Posner, A.S.: Conversion of amorphous calcium phosphate to microcrystalline hydroxyapatite. A pH-dependent, solution- mediated, solid-solid conversion. J. Phys. Chem. 77(19), 2313–2317 (1973)

    Article  CAS  Google Scholar 

  16. Ben-Nissan, B., Choi, A.H.: Calcium phosphate nanocoatings: production, physical and biological properties, and biomedical applications. Nanobioceramics for Healthcare Applications. 105–149 (2017)

  17. Macha, I.J., Boonyang, U., Cazalbou, S., Ben-Nissan, B., Charvillat, C., Oktar, F.N., Grossin, D.: Comparative study of Coral Conversion, Part 2: Microstructural evolution of calcium phosphate. J. Aust. Ceram. Soc. (2015)

  18. Eslami, H., Solati-Hashjin, M., Tahriri, M.: Synthesis and characterization of nano-hydroxyapatite (n-HAP) using the wet chemical technique. Int. J. Phys. Sci. 8(32), 1639–1645 (2013)

    Google Scholar 

  19. Al-Qasas, N.S., Rohani, S.: Synthesis of pure hydroxyapatite and the effect of synthesis conditions on its yield, crystallinity, morphology and mean particle size. Sep. Sci. Technol. 40(15), 3187–3224 (2005)

    Article  CAS  Google Scholar 

  20. Jiang, S.D., Yao, Q.Z., Ma, Y.F., Zhou, G.T., Fu, S.Q.: Phosphate-dependent morphological evolution of hydroxyapatite and implication for biomineralization. Gondw. Res. 28(2), 858–868 (2015)

    Article  CAS  Google Scholar 

  21. Liu, Q., Huang, S., Matinlinna, J.P., Chen, Z., Pan, H.: Insight into biological apatite: physicochemical properties and preparation approaches. Biomed. Res. Int. (2013)

  22. Mosmann, T.: Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 65(1–2), 55–63 (1983)

  23. Eslami, H., Solati-Hashjin, M., Tahriri, M.: Synthesis and characterization of hydroxyapatite nanocrystals via chemical precipitation technique. Iranian Journal of Pharmaceutical Sciences. 4(2), 127–134 (2008)

    Google Scholar 

  24. Rapacz-Kmita, A., Paluszkiewicz, C., Ślósarczyk, A., Paszkiewicz, Z.: FTIR and XRD investigations on the thermal stability of hydroxyapatite during hot pressing and pressureless sintering processes. J. Mol. Struct. 744, 653–656 (2005)

  25. Fuentes, G., Hernández, Y., Campos, Y., López, N., Rojas, M.L., Peón, E., Almirall, A., Delgado, J.A.: Composition influence on properties of acrylic composites loaded with synthetic hydroxyapatite. Lat. Am. Appl. Res. 38(2), 105–112 (2008)

  26. Smith, B.A., Ware, B.R.: Apparatus and methods for laser doppler electrophoresis. Contemporary Topics in Analytical and Clinical Chemistry. 2, 29–54 (1978)

    Article  CAS  Google Scholar 

  27. Miller, J.D., Yalamanchili, M.R., Kellar, J.J.: Surface charge of alkali halide particles as determined by laser-doppler electrophoresis. Langmuir. 8(5), 1464–1469 (1992)

    Article  CAS  Google Scholar 

  28. Ding, Z., Jiang, Y., Liu, X.: Nanoemulsions-based drug delivery for brain tumors. In: Nanotechnology-Based Targeted Drug Delivery Systems for Brain Tumors, pp. 327–358. Elsevier (2018)

    Google Scholar 

  29. Nimesh, S., Chandra, R., Gupta, N.: Advances in nanomedicine for the delivery of therapeutic nucleic acids. Woodhead Publishing. (2017)

  30. Joseph, E., Singhvi, G.: Multifunctional nanocrystals for cancer therapy: a potential nanocarrier. In: In: Nanomaterials for Drug Delivery and Therapy. Elsevier (2019)

    Google Scholar 

  31. Wei, K., Wang, Y., Lai, C., Ning, C., Wu, D., Wu, G., Zhao, N., Chen, X., Ye, J.: Synthesis and characterization of hydroxyapatite nanobelts and nanoparticles. Mater. Lett. 59, 2–3 (2005)

    Article  Google Scholar 

  32. Cengiz, B., Gokce, Y., Yildiz, N., Aktas, Z., Calimli, A.: Synthesis and characterization of hydroxyapatite nanoparticles. Colloids Surf. A Physicochem. Eng. Asp. 322(1–3), 29–33 (2008)

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to thank to their all collaborators for their useful suggestions to improve this paper.

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Authors and Affiliations

  1. Department of Metallurgical and Materials Engineering, Faculty of Chemical and Metallurgical Engineering, Yildiz Technical University, Istanbul, Turkey

    Gizem Mahmutoglu, Nilgun Kuskonmaz & Sibel Daglilar

  2. Center for Nanotechnology and Biomaterials Research, Marmara University, Marmara, Turkey

    Aysenur Topsakal, Eray Altan, Faik Nuzhet Oktar & Oguzhan Gunduz

  3. Department of Metallurgical and Materials Engineering, Faculty of Technology, Marmara University, Marmara, Turkey

    Eray Altan & Oguzhan Gunduz

  4. Department of Bioengineering, Faculty of Engineering, Marmara University, Marmara, Turkey

    Faik Nuzhet Oktar

  5. Department of Molecular Medicine, Aziz Sancar Institute of Experimental Medicine, Istanbul University, 34452, Istanbul, Turkey

    Gokce Erdemir

  6. Department of Physiology, Faculty of Medicine, Istanbul University, 34452, Istanbul, Turkey

    Serap Erdem Kuruca

  7. Department of Physiology, Istanbul University-Cerrahpasa, Istanbul, Turkey

    Sibel Akyol

  8. School of Life Sciences, Faculty of Science, University of Technology, Sydney, NSW, Australia

    Besim Ben-Nissan

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  1. Gizem Mahmutoglu
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  2. Aysenur Topsakal
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Correspondence to Faik Nuzhet Oktar, Oguzhan Gunduz or Besim Ben-Nissan.

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Mahmutoglu, G., Topsakal, A., Altan, E. et al. Effects of temperature and pH on the synthesis of nanohydroxyapatite powders by chemical precipitation. J Aust Ceram Soc 59, 1433–1441 (2023). https://doi.org/10.1007/s41779-023-00927-2

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