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Aqueous extract of Solanum macrocarpon Linn leaves abates hyperglycaemia and expression of glucose transporters gene in alloxan-induced diabetic rats

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Abstract

Purpose

In this study, antihyperglycaemic and level of gene expression of glucose transporters in alloxan-induced diabetic rats administered aqueous extract of S. macrocarpon leaves were assessed.

Method and results

Diabetes was induced by a single intraperitoneal (I.P) injection of freshly prepared alloxan. The animals were divided into six groups, euthanized on the fourteenth day of the experiment and different hyperglycaemic parameters were evaluated. Administration of different doses of the plant extract significantly (p < 0.05) reduced the fasting blood glucose level, glycated haemoglobin, serum lipid profiles, lipid peroxidation, and glucose-6-phosphatase. There was a significant (p < 0.05) increase in liver glycogen content, antioxidant enzyme activities, hexokinase activity, and expression of glucose transporter genes (GLUT-2 and GLUT-4) in diabetic rats administered different doses of S. macrocarpon.

Conclusion

It can be concluded that the aqueous extract of S. macrocarpon leaves could be helpful in the management of diabetes mellitus and its metabolic complications.

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References

  1. Nelson JE, Cox CE, Hope AA, Carson SS (2010) Chronic critical illness. Am J Respir Crit Care Med 182(4):446–454

    Article  Google Scholar 

  2. Rambabu K, Kumar PS, Kameswari P (2014) Textbook of biochemistry, 2nd edn. AITBS Publishers, New Delhi

    Google Scholar 

  3. Abdalla HI, Abugabal NS, Hassan AZ, El-Safty MM, Shalaby NMM (2012) Antiviral activity of Aloe hijazensis against some hemagglutinating viruses infection and its phytoconstituents. Arch Pharmacal Res 35:1347–1354

    Article  CAS  Google Scholar 

  4. Denton OA, Nielsen I, Grubben GJH (2004) Plant resources of Tropical Africa 2. Nord J Bot 23:298–298

    Google Scholar 

  5. Onuora EEO, Okafor CN (2016) Efficacy of extracts of African eggplant and Okra leaves on alloxan-induced diabetes mellitus adult male albino rats. Pak J Nutr 15:551–555

    Article  CAS  Google Scholar 

  6. Adewale OB, Onasanya A, Fadaka AO, Iwere H, Anadozie SO, Osukoya OA, Olayide II (2014) In vitro antioxidant effect of aqeous extract of Solanum macrocarpon leaves in rat liver and brain. Oxid Antioxid Med Sci 3:225–229

    Article  Google Scholar 

  7. Dougnon TV, Bankolé HS, Klotoé JR, Sènou M, Fah L, Koudokpon H, Akpovi C, Dougnon TJ, Addo P, Loko F, Boko M (2014) Treatment of hypercholesterolemia: screening of Solanum macrocarpon Linn (Solanaceae) as a medicinal plant in Benin. Avicenna J Phytomed 4:160–169

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Ajiboye BO, Akalabu MC, Ojo OA, Afolabi OB, Okesola MA, Olayide I, Oyinloye BE (2018) Inhibitory effect of ethyl acetate fraction of Solanummacrocarpon L. leaves on cholinergic, monoaminergic, and purinergic enzyme activities. J Food Biochem 42:e12643

    Article  Google Scholar 

  9. Ajiboye BO, Ojo OA, Adeyonu O, Imiere O, Olayide I, Fadaka A, Oyinloye BE (2016) Inhibitory effect on key enzymes relevant to acute type-2 diabetes and antioxidative activity of ethanolic extract of Artocarpus heterophyllus stem bark. J Acute Dis 5:423–429

    Article  Google Scholar 

  10. Islam SM, Niessen LW, Seissler J, Ferrari U, Biswas T, Islam A, Lechner A (2015) Diabetes knowledge and glycemic control among patients with type 2 diabetes in Bangladesh. SpringerPlus 4:284

    Article  Google Scholar 

  11. Ojo O, Ajiboye BO, Oluwatosin DI, Adeyonu O, Olayide I, Fadaka AO (2018) Antioxidative properties of Blighiasapida K. D. Koenig stem bark extract and inhibitory effects on carbohydrate hydrolyzing enzymes associated with non-insulin dependent diabetes mellitus. Pharmacogn J 10:376–383

    Article  CAS  Google Scholar 

  12. Ahmad MS, Pischetsrieder M, Ahmed N (2007) Aged garlic extract and S-allyl cysteine prevent formation of advanced glycation endproducts. Eur J Pharmacol 561:32–38

    Article  CAS  Google Scholar 

  13. Eastham RD (1985) Biochemical values in clinical medicine, 7th edn. John Wright and Sons, Bristol

    Google Scholar 

  14. Tietz NW (1995) Clinical guide to laboratory tests, 3rd edn. W.B. Saunders Company, Philadelphia, pp 518–519

    Google Scholar 

  15. Trinder P (1969) Estimation of triglyceride in blood GPO-PAP enzymatic method. Am J Clin Biochem 6:24–27

    Article  CAS  Google Scholar 

  16. Friedewald WT, Levy RI, Fredrickson DS (1972) Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 18:499–502

    Article  CAS  Google Scholar 

  17. Akinyosoye F, Fawole M, Akinyanju J (1987) Studies on some enzymes of carbohydrate metabolism in Geotrichum candidum. Niger J Microbiol 7:154–161

    Google Scholar 

  18. Baginski SE, Foà PP, Zak B (1974) Glucose-6-phosphatase. Methoden der enzymatischen Analyse 1:839–843

    Google Scholar 

  19. Elekofehinti OO, Ariyo EO, Akinjiyan OS, Lawal AO, Adanlawo IG, Joao BL (2018) Potential use of bitter melon (Momordica charantia) derived compounds as antidiabetics: in silco and in vitro studies. Pathiophysiology 25:327–333

    Article  CAS  Google Scholar 

  20. Holness MJ, Palmer TN, Sugden MC (1988) Suppression of hepatic glucose output after glucose re-feeding in the gsd/gsd rat. Biochem J 252:928–929

    Article  CAS  Google Scholar 

  21. Varshney R, Kate R (1990) Effects of calmodulin antagonists on radiation-induced lipid peroxidation in microsomes. Int J Radiat Biol 58:733–743

    Article  CAS  Google Scholar 

  22. Misra HP, Fridovich I (1972) The role of superoxide anion in the autooxidation of epinephrine and simple assay for superoxide dismutase. J Biol Chem 247:3170–3175

    Article  CAS  Google Scholar 

  23. Sinha AK, Kurtz M, Bhattacharjee JK (1971) Effect of hydroxylysine on the biosynthesis of lysine in saccharomyces. J Bacteriol 108:715–719

    Article  CAS  Google Scholar 

  24. Paglia DE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169

    CAS  PubMed  Google Scholar 

  25. Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 50:536–546

    Google Scholar 

  26. DeFronzo RA, Bonadonna RC, Ferrannini E (1992) Pathogenesis of non insulin dependent diabetes mellitus. Diabetes Care 1992(3):318–368

    Article  Google Scholar 

  27. Mironova MA, Klein RL, Virella GT, Lopes-Virella MF (2000) Antimodified LDL antibodies, LDL-containing immune complexes and susceptibility of LDL to in vitro oxidation in patients with type-2 diabetes. Diabetes 49:1033–1041

    Article  CAS  Google Scholar 

  28. Martirosyan DM, Miroshnichenko LA, Kulakova SN, Pogojeva AV, Zoloedov VI (2007) Amaranth oil application for coronary heart disease and hypertension. Lipids Health Dis 6:1

    Article  Google Scholar 

  29. Zicha J, Kunes J, Devynck MA (1999) Abnormalities of membrane function and lipid metabolism in hypertension. A review. Am J Hypertens 12:315–331

    Article  CAS  Google Scholar 

  30. Grover JK, Vats V, Rathi SS (2000) Anti-hyperglycemic effect of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol 73:461–470

    Article  CAS  Google Scholar 

  31. Lekshmi RK, Rajesh R, Mini S (2015) Ethyl acetate fraction of Cissus quadrangularis stem ameliorates hyperglycaemia-mediated oxidative stress and suppresses inflammatory response in nicotinamide/streptozotocin induced type 2 diabetic rats. Phytomedicine 22:952–960

    Article  CAS  Google Scholar 

  32. Yechoor VK, Patti ME, Saccone R, Kahn CR (2002) Coordinated pattern of gene expression for substrate and energy metabolism in skeletal muscle of diabetic mice. Proc Natl Acad Sci USA 99:10587–10592

    Article  CAS  Google Scholar 

  33. Kim S, Gailite I, Moussian B, Luschnig S, Goette M, Fricke K, Honemann-Capito M, Grubmüller H, Wodarz A (2009) Kinase-activity-independent functions of a typical protein kinase C in Drosophila. J Cell Sci 122:3759–3771

    Article  CAS  Google Scholar 

  34. Naik P (2011) Biochemistry textbook, 3rd edn. Jaypee Brothers Medical Publishers Ltd, New Delhi

    Google Scholar 

  35. Raju J, Gupta D, Rao AR, Yadava PK, Baquer NZ (2001) Trigonell afoenum graecum (fenugreek) seed powder improves glucose homeostasis in alloxan-diabetic rat tissues by reversing the altered glycolytic, gluconeogenic and lipogenic enzymes. Mol Cell Biochem 224:45–51

    Article  CAS  Google Scholar 

  36. Kumar V, Ahmed D, Verma A, Anwar F, Ali M, Mujeeb M (2013) Umbelliferone β-d-galactopyranoside from Aeglemarmelos (L.) corr. an ethnomedicinal plant with antidiabetic, antihyperlipidemic and antioxidative activity. BMC Complement Altern Med 13:273

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors wish to express their gratitude to the support received by the South African Medical Research Council (SAMRC) through funding received from the South African National Treasury. Also, its contents are mainly the responsibility of the authors and do not necessarily represent the official views of the South African Medical Research Council.

Funding

This study was supported by the South African Medical Research Council through funding collected from the South African National Treasury.

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Author notes

  1. O. A. Ojo

    Present address: Department of Biochemistry, Landmark University, Omu-Aran, Kwara State, Nigeria

Authors and Affiliations

  1. Phytomedicine, Biochemical Toxicology and Biotechnology Laboratory, Department of Biochemistry, College of Sciences, Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria

    B. O. Ajiboye, B. E. Oyinloye, O. S. Owero-ozeze, I. L. Ekakitie & O. A. Ojo

  2. Biotechnology and Structural Biology (BSB) Group, Department of Biochemistry and Microbiology, University of Zululand, KwaDlangezwa, 3886, South Africa

    B. E. Oyinloye

  3. Department of Biochemistry, Covenant University, Ota, Ogun State, Nigeria

    M. A. Okesola

  4. Molecular Biophysics and Structural Biology Group, Department of Biochemistry, Faculty of Science, University of Johannesburg, Johannesburg, South Africa

    A. P. Kappo

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Correspondence to B. O. Ajiboye.

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This study was done following the Animal Ethical Committee of Afe Babalola University, Ado-Ekiti, Ekiti State, Nigeria.

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Ajiboye, B.O., Oyinloye, B.E., Owero-ozeze, O.S. et al. Aqueous extract of Solanum macrocarpon Linn leaves abates hyperglycaemia and expression of glucose transporters gene in alloxan-induced diabetic rats. J Endocrinol Invest 44, 265–276 (2021). https://doi.org/10.1007/s40618-020-01280-y

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