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Functional Peptides from One-bead One-compound High-throughput Screening Technique

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Abstract

Combinatorial chemistry provides a cost-effective method for the rapid discovery of new functional peptides. One-bead one-compound(OBOC) high-throughput screening technique offers a lot of structurally diverse peptides to be rapidly synthesized and screened for binding to a target of interest. The OBOC peptide library screening involves three main steps: library construction, positive beads separation, and peptide sequencing. This review mainly summarizes some special technique tips during functional peptide screening and potential future directions of the OBOC high-throughput screening technique.

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Acknowledgements

This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences(No.XDB36000000) and the National Natural Science Foundation of China(Nos.8201101351, 51890891, 51890892, 51890894).

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Authors and Affiliations

  1. CAS Center for Excellence in Nanoscience, CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology(NCNST), Beijing, 100190, P. R. China

    Yaqiong Yan, Lei Wang & Hao Wang

  2. Center of Materials Science and Optoelectronics Engineering, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Yaqiong Yan, Lei Wang & Hao Wang

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  1. Yaqiong Yan
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  2. Lei Wang
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  3. Hao Wang
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Correspondence to Hao Wang.

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Yan, Y., Wang, L. & Wang, H. Functional Peptides from One-bead One-compound High-throughput Screening Technique. Chem. Res. Chin. Univ. 39, 83–91 (2023). https://doi.org/10.1007/s40242-023-2356-2

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