Skip to main content
SpringerLink
Log in

Hair growth-promoting effect of human dermal stem/progenitor cell-derived conditioned medium

  • Original Article
  • Published:
Tissue Engineering and Regenerative Medicine Aims and scope

Abstract

We previously reported that human dermal stem/progenitor cell-derived conditioned medium (hDSPC-CM) mediates skin regeneration effects, such as wound healing and reducing the number of UVA-irradiated apoptotic cell population. Here we demonstrate the effects of hDSPC-CM on the hair re-growth. hDSPC-CM was used for study regarding the activation of alkaline phosphatase (ALP) activity and the up-regulation of mRNA expression for dermal papilla cell markers, such as SOX2, S100ß, VCAN, and CORIN. And the secretion of Wnt3a protein from hDSPC-CM was significantly increased compared to non hDSPC-CM. Wnt3a protein can restore ALP activity significantly. Furthermore we investigated the effect of hDSPC-CM on hair regeneration of C57BL/6 mice by considering the relationship between Wnt/ß-catenin pathway and hair formation. Taken together, these data suggest that hDSPC-CM can exert some beneficial effects on hair regeneration and may be used as a therapeutic agent for curing hair loss.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Sengel P, Mauger A. Peridermal cell patterning in the feather-forming skin of the chick embryo. Dev Biol 1976;51:166–171.

    Article  CAS  PubMed  Google Scholar 

  2. Shimizu H, Morgan BA. Wnt signaling through the beta-catenin pathway is sufficient to maintain, but not restore, anagen-phase characteristics of dermal papilla cells. J Invest Dermatol 2004;122:239–245.

    Article  CAS  PubMed  Google Scholar 

  3. Fuchs E, Merrill BJ, Jamora C, DasGupta R. At the roots of a never-ending cycle. Dev Cell 2001;1:13–25.

    Article  CAS  PubMed  Google Scholar 

  4. Paus R, Müller-Röver S, Van Der Veen C, Maurer M, Eichmüller S, Ling G, et al. A comprehensive guide for the recognition and classification of distinct stages of hair follicle morphogenesis. J Invest Dermatol 1999;113:523–532.

    Article  CAS  PubMed  Google Scholar 

  5. Schneider MR, Schmidt-Ullrich R, Paus R. The hair follicle as a dynamic miniorgan. Curr Biol 2009;19:R132–R142.

    Article  CAS  PubMed  Google Scholar 

  6. Paus R, Cotsarelis G. The biology of hair follicles. N Engl J Med 1999;341:491–497.

    Article  CAS  PubMed  Google Scholar 

  7. D’Amico AV, Roehrborn CG. Effect of 1 mg/day finasteride on concentrations of serum prostate-specific antigen in men with androgenic alopecia: a randomised controlled trial. Lancet Oncol 2007;8:21–25.

    Article  PubMed  Google Scholar 

  8. Jeong YM, Sung YK, Kim WK, Kim JH, Kwack MH, Yoon I, et al. Ultraviolet B preconditioning enhances the hair growth-promoting effects of adipose-derived stem cells via generation of reactive oxygen species. Stem Cells Dev 2013;22:158–168.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  9. Park BS, Kim WS, Choi JS, Kim HK, Won JH, Ohkubo F, et al. Hair growth stimulated by conditioned medium of adipose-derived stem cells is enhanced by hypoxia: evidence of increased growth factor secretion. Biomed Res 2010;31:27–34.

    Article  CAS  PubMed  Google Scholar 

  10. Chen L, Tredget EE, Wu PY, Wu Y. Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and enhance wound healing. PLoS One 2008;3:e1886.

    Article  Google Scholar 

  11. Shim JH, Park JY, Lee MG, Kang HH, Lee TR, Shin DW. Human dermal stem/progenitor cell-derived conditioned medium ameliorates ultraviolet a-induced damage of normal human dermal fibroblasts. PLoS One 2013;8:e67604.

    Article  Google Scholar 

  12. Huh MI, Kim MS, Kim HK, Lim JO. Effect of conditioned media collected from human amniotic fluid-derived stem cells (hAFSCs) on skin regeneration and photo-aging. Tissue Eng Regen Med 2014;11:171–177.

    Article  Google Scholar 

  13. Park PJ, Moon BS, Lee SH, Kim SN, Kim AR, Kim HJ, et al. Hair growth-promoting effect of Aconiti Ciliare Tuber extract mediated by the activation of Wnt/ß-catenin signaling. Life Sci 2012;91:935–943.

    Article  CAS  PubMed  Google Scholar 

  14. Gat U, DasGupta R, Degenstein L, Fuchs E. De Novo hair follicle morphogenesis and hair tumors in mice expressing a truncated beta-catenin in skin. Cell 1998;95:605–614.

    Article  CAS  PubMed  Google Scholar 

  15. Huelsken J, Vogel R, Erdmann B, Cotsarelis G, Birchmeier W. beta-Catenin controls hair follicle morphogenesis and stem cell differentiation in the skin. Cell 2001;105:533–545.

    Article  CAS  PubMed  Google Scholar 

  16. Lowry WE, Blanpain C, Nowak JA, Guasch G, Lewis L, Fuchs E. Defining the impact of beta-catenin/Tcf transactivation on epithelial stem cells. Genes Dev 2005;19:1596–1611.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  17. Myung PS, Takeo M, Ito M, Atit RP. Epithelial Wnt ligand secretion is required for adult hair follicle growth and regeneration. J Invest Dermatol 2013;133:31–41.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  18. Chen J, Roop DR. Mimicking hair disorders by genetic manipulation of organ-cultured human hair follicles. J Invest Dermatol 2012;132:2312–2314.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  19. Shim JH, Kang HH, Lee TR, Shin DW. Enrichment and characterization of human dermal stem/progenitor cells using collagen type IV. J Dermatol Sci 2012;67:202–205.

    Article  CAS  PubMed  Google Scholar 

  20. Shim JH, Lee TR, Shin DW. Novel in vitro culture condition improves the stemness of human dermal stem/progenitor cells. Mol Cells 2013;36:556–563.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  21. Handjiski BK, Eichmüller S, Hofmann U, Czarnetzki BM, Paus R. Alkaline phosphatase activity and localization during the murine hair cycle. Br J Dermatol 1994;131:303–310.

    Article  CAS  PubMed  Google Scholar 

  22. McElwee KJ, Kissling S, Wenzel E, Huth A, Hoffmann R. Cultured peribulbar dermal sheath cells can induce hair follicle development and contribute to the dermal sheath and dermal papilla. J Invest Dermatol 2003;121:1267–1275.

    Article  CAS  PubMed  Google Scholar 

  23. Müller-Röver S, Handjiski B, van der Veen C, Eichmüller S, Foitzik K, McKay IA, et al. A comprehensive guide for the accurate classification of murine hair follicles in distinct hair cycle stages. J Invest Dermatol 2001;117:3–15.

    Article  PubMed  Google Scholar 

  24. Ohyama M, Zheng Y, Paus R, Stenn KS. The mesenchymal component of hair follicle neogenesis: background, methods and molecular characterization. Exp Dermatol 2010;19:89–99.

    Article  PubMed  Google Scholar 

  25. Filali M, Cheng N, Abbott D, Leontiev V, Engelhardt JF. Wnt-3A/beta-catenin signaling induces transcription from the LEF-1 promoter. J Biol Chem 2002;277:33398–33410.

    Article  CAS  PubMed  Google Scholar 

  26. Lee SH, Yoon J, Shin SH, Zahoor M, Kim HJ, Park PJ, et al. Valproic acid induces hair regeneration in murine model and activates alkaline phosphatase activity in human dermal papilla cells. PLoS One 2012;7:e34152.

    Article  Google Scholar 

  27. Greco V, Chen T, Rendl M, Schober M, Pasolli HA, Stokes N, et al. A two-step mechanism for stem cell activation during hair regeneration. Cell Stem Cell 2009;4:155–169.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  28. Grose R, Fantl V, Werner S, Chioni AM, Jarosz M, Rudling R, et al. The role of fibroblast growth factor receptor 2b in skin homeostasis and cancer development. EMBO J 2007;26:1268–1278.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  29. Guo L, Degenstein L, Fuchs E. Keratinocyte growth factor is required for hair development but not for wound healing. Genes Dev 1996;10:165–175.

    Article  CAS  PubMed  Google Scholar 

  30. Ramos R, Guerrero-Juarez CF, Plikus MV. Hair follicle signaling networks: a dermal papilla-centric approach. J Invest Dermatol 2013;133:2306–2308.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  31. Callahan CA, Ofstad T, Horng L, Wang JK, Zhen HH, Coulombe PA, et al. MIM/BEG4, a Sonic hedgehog-responsive gene that potentiates Gli-dependent transcription. Genes Dev 2004;18:2724–2729.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  32. Mill P, Mo R, Fu H, Grachtchouk M, Kim PC, Dlugosz AA, et al. Sonic hedgehog-dependent activation of Gli2 is essential for embryonic hair follicle development. Genes Dev 2003;17:282–294.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  33. St-Jacques B, Dassule HR, Karavanova I, Botchkarev VA, Li J, Danielian PS, et al. Sonic hedgehog signaling is essential for hair development. Curr Biol 1998;8:1058–1068.

    Article  CAS  PubMed  Google Scholar 

  34. Botchkarev VA, Botchkareva NV, Roth W, Nakamura M, Chen LH, Herzog W, et al. Noggin is a mesenchymally derived stimulator of hair-follicle induction. Nat Cell Biol 1999;1:158–164.

    Article  CAS  PubMed  Google Scholar 

  35. Kulessa H, Turk G, Hogan BL. Inhibition of Bmp signaling affects growth and differentiation in the anagen hair follicle. EMBO J 2000;19:6664–6674.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  36. Rendl M, Polak L, Fuchs E. BMP signaling in dermal papilla cells is required for their hair follicle-inductive properties. Genes Dev 2008;22:543–557.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  37. Woo DH, Kim SK, Lim HJ, Heo J, Park HS, Kang GY, et al. Direct and indirect contribution of human embryonic stem cell-derived hepatocyte-like cells to liver repair in mice. Gastroenterology 2012;142:602–611.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Oriental Cosmetic Science, Semyung University, Jecheon, Korea

    Joong Hyun Shim

Authors
  1. Joong Hyun Shim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joong Hyun Shim.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shim, J.H. Hair growth-promoting effect of human dermal stem/progenitor cell-derived conditioned medium. Tissue Eng Regen Med 12, 268–275 (2015). https://doi.org/10.1007/s13770-015-0012-8

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13770-015-0012-8

Key Words

Search

Navigation