Abstract
Heat shock proteins or Hsps are critical in mounting plant resistance against heat stress. The complex Hsp spectrum of Arabidopsis thaliana plant contains over two hundred proteins belonging to six different families namely Hsp20, Hsp40, Hsp60, Hsp70, Hsp90 and Hsp100. Importantly, the cellular function(s) of most Hsps remains to be established. We aimed at phenotyping of stress and development response of the selected, homozygous hsp mutant lines produced by T-DNA insertional mutagenesis method. The heat stress phenotype was assessed for basal and acquired heat stress response at seed and seedling stages. Distinct phenotype was noted for the hot1-3 mutant (knockout mutant of Hsp101 gene) showing higher heat sensitivity and for the salk_087844 mutant (knockout mutant of Hsc70-2 gene) showing higher heat tolerance than the wild type seedlings. The homozygous cs808162 mutant (mutant of ClpB-p gene encoding for the chloroplast-localized form of Hsp101) did not survive even under unstressed, control condition. salk_064887C mutant (mutant of cpn60β4 gene) showed accelerated development cycling. The hot1-3 mutant apart from showing different heat response, exhibited development lesions like bigger size of seeds, buds, siliques, and pollen compared to the wild type plants. In response to controlled deterioration treatment of seeds, hot1-3 seeds showed higher accumulation of reactive oxygen species molecules, higher rates of protein and lipid oxidation and a faster decline in germination rate as compared to wild type seeds. Our findings show that Hsps perform diverse metabolic functions in plant response to stress, growth, and development.
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Abbreviations
- APX :
-
Ascorbate peroxidase
- AT :
-
Acquired tolerance
- BT :
-
Basal tolerance
- CD :
-
Controlled deterioration
- Clp :
-
Caseinolytic proteases
- DPS :
-
Days post stress
- GI :
-
Germination index
- HS :
-
Heat stress
- Hsf :
-
Heat shock factor
- Hsp :
-
Heat shock protein
- HSR :
-
Heat stress response
- KD :
-
Knockdown
- KO :
-
Knockout
- MDG :
-
Multiplying mean daily germination
- PCR :
-
Polymerase chain reaction
- PQC :
-
Protein quality control
- PV :
-
Peak value
- ROS :
-
Reactive oxygen species
- SOD :
-
Superoxide dismutase
- TAIR :
-
The Arabidopsis information resource
- UTR :
-
Untranslated region
- WT :
-
Wild type
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Acknowledgements
LDT and RK acknowledge the University Grants Commission (UGC), Government of India New Delhi for the Ph.D. fellowship award. AG acknowledges financial support from the Center for Advanced Research and Innovation on Plant Stress and Developmental Biology, Department of Biotechnology (DBT), Government of India and J.C. Bose Fellowship award grant from Science and Engineering Research Board (SERB), Government of India.
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Tiwari, L.D., Kumar, R., Sharma, V. et al. Stress and development phenotyping of Hsp101 and diverse other Hsp mutants of Arabidopsis thaliana. J. Plant Biochem. Biotechnol. 30, 889–905 (2021). https://doi.org/10.1007/s13562-021-00706-9
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DOI: https://doi.org/10.1007/s13562-021-00706-9