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Expression of the broccoli catalase gene (BoCAT) enhances heat tolerance in transgenic Arabidopsis

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Abstract

The objective of this study was to transfer catalase gene (CAT1 and CAT2) complementary (c)DNAs under the control of a ubiquitin promoter into Arabidopsis via Agrobacterium-mediated transformation. A real-time polymerase chain reaction analysis demonstrated that both the BoCAT1 and BoCAT2 genes were overexpressed in transgenic Arabidopsis thaliana (At). The activity of CAT in the AtCAT2-2 transgenic line was 6-fold higher than that of the non-transgenic plant under heat stress, and the CAT amount in the AtCAT2-2 line also highly accumulated according to a Western blot analysis. Compared to non-transgenic Arabidopsis plants, a lower level of heat-induced H2O2 accumulation was detected by diaminobenzidine staining in leaves of transgenic plants with a high level of CAT activity, indicating that overexpression of BoCAT in Arabidopsis could enhance the heat tolerance by eliminating H2O2. This is the first report suggesting that CAT-encoding gene expression in Arabidopsis is regulated by heat stress.

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Abbreviations

CAT:

Catalase

BoCAT :

Broccoli catalase gene

At:

Arabidopsis thaliana

ROS:

Reactive oxygen species

HPT:

Hygromycin phosphotransferase

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Authors and Affiliations

  1. Department of Biotechnology, Ming Chuan University, Taoyuan 333, Taiwan, Republic of China

    Chih-Ming Chiang

  2. Graduate Institute of Plant Biology, National Taiwan University, Taipei 106, Taiwan, Republic of China

    Shi-Peng Chen

  3. Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan, Republic of China

    Long-Fang O. Chen

  4. Department of Life Science, Fu Jen Catholic University, New Taipei City 242, Taiwan, Republic of China

    Ming-Chang Chiang

  5. Graduate Institute of Biotechnology, Chinese Culture University, Taipei 111, Taiwan, Republic of China

    Hao-Lun Chien & Kuan-Hung Lin

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  1. Chih-Ming Chiang
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  2. Shi-Peng Chen
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  3. Long-Fang O. Chen
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  4. Ming-Chang Chiang
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  5. Hao-Lun Chien
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Correspondence to Kuan-Hung Lin.

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C.-M. Chiang and S.-P. Chen contributed equally to this study.

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Chiang, CM., Chen, SP., Chen, LF.O. et al. Expression of the broccoli catalase gene (BoCAT) enhances heat tolerance in transgenic Arabidopsis . J. Plant Biochem. Biotechnol. 23, 266–277 (2014). https://doi.org/10.1007/s13562-013-0210-1

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  • DOI: https://doi.org/10.1007/s13562-013-0210-1

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