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Environmentally friendly synthesis of silver nanoparticles using Prickly Pear extract and their antimicrobial and antioxidant activities

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Abstract

The green routs for the synthesis of silver nanoparticles (AgNPs) and its multiple applications have attracted many researchers, because silver nanoparticles synthesized by the green method, in addition to being environmentally friendly, are effective in targeting specific tissues and pathogenic microorganisms. The fruit of the Prickly Pear plant has a wide range of secondary metabolites with high regenerative power that can be used for the biosynthesis of AgNPs. Therefore; in this study, green synthesis of nanoparticles was performed using Cactus fruit extract and its antioxidant and antibacterial properties were examined. Antimicrobial activity of extracts and AgNPs against standard strains of gram-positive bacteria such as Staphylococcus aureus (PTCC 16538), Enterococcus faecalis (ATCC 15753), Streptococcus mutans (ATCC 35668), Streptococcus mitis (ATCC 6249), Klebsiella pneumoniae (PTCC 700603), Staphylococcus epidermidis (ATCC 12228) as well as gram-negative Escherichia coli (ATCC 25922) and Pseudomonas aeruginosa (ATCC 27853) were determined by micro-broth dilution method. SEM, UV–Vis, EDAX and XRD techniques confirm successful biosynthesis of silver nanoparticles and average particle size was around 40–65 nm. Silver nanoparticles acted as an inhibitor of DPPH radicals and showed desirable antioxidant properties. MIC and MBC values of experimental pathogens were recorded in the range of 2.34–18.75 µg/mL and 2.34–37.5 µg/mL, respectively. The results showed appropriate antibacterial and antioxidant activity of biosynthesized silver nanoparticles. Therefore, the synthesized silver nanoparticles can be used as natural resources to produce antioxidant and antimicrobial supplements in the pharmaceutical industry.

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Data availability

The data that support the findings of this study are available on request from the corresponding author.

Abbreviations

AgNPs:

Silver nanoparticles

PTCC 16538:

Persian Type Culture Collection Staphylococcus aureus

ATCC 15753:

American Type Culture Collection Enterococcus faecalis

ATCC 35668:

Streptococcus mutans

ATCC 6249:

Streptococcus mitis

PTCC 700603:

Klebsiella pneumoniae

ATCC 12228:

Staphylococcus epidermidis

ATCC 25922:

Escherichia coli

ATCC 27853:

Pseudomonas aeruginosa

AgNO3 :

Silver nitrate

DPPH:

Diphenyl-1-picrylhydrazyl

XRD:

X-ray diffraction

SEM:

Scanning electron microscopy

EDAX:

X-ray energy diffraction

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Acknowledgements

We thank the support of Birjand University of Medical Sciences (BUMS) for the success of this article (Code: 5309).

Funding

This research project was funded by Birjand University of medical sciences.

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Authors and Affiliations

  1. Department of Hematology, School of Allied Medical Sciences, Birjand University of Medical Sciences, Birjand, Iran

    Majid Zare Bidaki

  2. Medical Toxicology and Drug Abuse Research Center (MTDRC), Birjand University of Medical Sciences (BUMS), Birjand, Iran

    Ali Naghizadeh, Ali Yousefinia, Mostafa Hosseinzadeh, Sajad Lashkari & Sobhan Mortazavi-Derazkola

  3. Department of Environmental Health Engineering, Faculty of Health, Birjand University of Medical Sciences, Birjand, Iran

    Marzieh Moghanni

Authors
  1. Majid Zare Bidaki
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  2. Ali Naghizadeh
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  3. Ali Yousefinia
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  4. Mostafa Hosseinzadeh
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  5. Sajad Lashkari
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Contributions

Ali Naghizadeh was the supervisor of this research project, all of other authors contributed equally in performing this research project.

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Correspondence to Ali Naghizadeh.

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This paper was approved on BUMS ethical committee with code IR.BUMS.REC.1400.003.

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Bidaki, M.Z., Naghizadeh, A., Yousefinia, A. et al. Environmentally friendly synthesis of silver nanoparticles using Prickly Pear extract and their antimicrobial and antioxidant activities. Biomass Conv. Bioref. (2024). https://doi.org/10.1007/s13399-023-05259-6

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