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Combined treatment of ginsenoside Rg2 and piceatannol mixture reduces the apoptosis and DNA damage induced by UVB in HaCaT cells

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Abstract

Background

Ultraviolet B (UVB), a major cause of skin photoaging, leads to DNA damage directly and indirectly in skin cells. DNA damage can contribute to aging by increasing apoptosis, cellular senescence and cell dysfunction. To prevent photoaging and rejuvenate photoaged skin, combinations of active compounds from natural products are used for medicine and cosmetic ingredients.

Objective

In this study, we investigated the synergistic effects of Rg2 and piceatannol (PIC) mixture (RP) on protecting skin against UVB-induced DNA damage in HaCaT cells.

Results

Cells treated with RP for 24 h post UVB exposure increased the cell viability in a concentration-dependent manner compared to non-treated control. In DPPH and DCF-DA assay, RP showed similar free radical scavenging activity to that of single-treatment PIC. RP more decreased the levels of CPD, DDR proteins including p53, p-p53 and p21 and apoptosis-related proteins, such as Bax, Bim, and cleaved caspase-7 than that of single-treatment PIC or Rg2. Also, RP decreased more the mRNA level of MMP-1, -3 and -9 than that of single-treatment PIC or Rg2.

Conclusion

It is inferred that Rg2 promotes DNA repair by regulating genes related with DDR and PIC promotes DNA repair by effectively scavenging UVB-induced ROS through high antioxidant activity. Enhanced DNA repair by RP leads to protecting skin from UVB-induced skin photoaging.

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References

  • Alessio N et al (2021) Biomolecular evaluation of piceatannol’s effects in counteracting the senescence of mesenchymal stromal cells: a new candidate for senotherapeutics? Int J Mol Sci 22:11619

    Article  PubMed  PubMed Central  Google Scholar 

  • Barzilai A, Yamamoto K (2004) DNA damage responses to oxidative stress. DNA Repair (Amst) 3:1109–1115

    Article  CAS  PubMed  Google Scholar 

  • Chae S et al (2010) A comparative study of the potential antioxidant activities of ginsenosides. J Food Biochem 34:31–43

    Article  Google Scholar 

  • Chung YH et al (2018) Protective effects of ginsenoside Rg2 and astaxanthin mixture against UVB-induced DNA damage. Anim Cells Syst (Seoul) 22:400–406

    Article  CAS  PubMed  Google Scholar 

  • Cordova-Gomez M, Galano A, Alvarez-Idaboy JR (2013) Piceatannol, a better peroxyl radical scavenger than resveratrol. RSC Adv 43:20209–20218

    Article  Google Scholar 

  • Crochemore C et al (2002) Enhancement of p53 activity and inhibition of neural cell proliferation by glucocorticoid receptor activation. FASEB J 16:761–770

    Article  CAS  PubMed  Google Scholar 

  • de Jager TL, Cockrell AE, Du Plessis SS (2017) Ultraviolet light induced generation of reactive oxygen species. Adv Exp Med Biol 996:15–23

    Article  PubMed  Google Scholar 

  • Emri G, Horkay I, Remenyik E (2006) The role of free radicals in the UV-induced skin damage. Orv Hetil 147:731–735

    PubMed  Google Scholar 

  • Fu W et al (2015) Protective effects of ginsenoside Rg2 against H2O2-induced injury and apoptosis in H9c2 cells. Int J Clin Exp Med 8:19938–19947

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ha SE et al (2010) Effects of ginsenoside Rg2 on the ultraviolet B-induced DNA damage responses in HaCaT cells. Naunyn Schmiedebergs Arch Pharmacol 382:89–101

    Article  CAS  PubMed  Google Scholar 

  • Herzinger T et al (1995) Ultraviolet B irradiation-induced G2 cell cycle arrest in human keratinocytes by inhibitory phosphorylation of the ccd2 cell cycle kinase. Oncogene 11:2151–2156

    CAS  PubMed  Google Scholar 

  • Jeong SJ et al (2007) Effects of mRg2, a mixture of ginsenosides containing 60% Rg2, on ultraviolet B-induced DNA repair synthesis and apoptosis in NIH3T3 cells. Int J Toxicol 26:151–158

    Article  CAS  PubMed  Google Scholar 

  • Jin GH et al (2007) UVB induced oxidative stress in human keratinocytes and protective effect of antioxidant agents. Radiat Environ Biophys 46:61–68

    Article  CAS  PubMed  Google Scholar 

  • Kang HJ et al (2016) Stereospecificity of ginsenoside Rg2 epimers in the protective response against UV-B radiation-induced oxidative stress in human epidermal keratinocytes. J Photochem Photobiol B 165:232–239

    Article  CAS  PubMed  Google Scholar 

  • Karra AG et al (2018) Potential dissociative glucocorticoid receptor activity for protopanaxadiol and protopanaxatriol. Int J Mol Sci 20:94

    Article  PubMed  PubMed Central  Google Scholar 

  • Kershaw J, Kim KH (2017) The therapeutic potential of piceatannol, a natural stilbene, in metabolic diseases: a review. J Med Food 20:427–438

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Lee SJ et al (2016) Changes in the ginsenoside content duringfermentation using an appliance for the preparation of red ginseng. Am J Chin Med 44:1595–1606

    Article  CAS  PubMed  Google Scholar 

  • Li H et al (2012) Glucocorticoid receptor and sequential p53 activation by dexamethasone mediates apoptosis and cell cycle arrest of osteoblastic MC3T3-E1 cells. PLoS One 7:e37030

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Li Z et al (2022) Ginsenosides repair UVB-induced skin barrier damage in BALB/c hairless mice and HaCaT keratinocytes. J Ginseng Res 46:115–125

    Article  PubMed  Google Scholar 

  • Lo HL et al (2005) Differential biologic effects of CPD and 6–4PP UV-induced DNA damage on the induction ofapoptosis and cell-cycle arrest. BMC cancer 5:135

    Article  PubMed  PubMed Central  Google Scholar 

  • Lü JM, Yao Q, Chen C (2009) Ginseng compounds: an update on their molecular mechanisms and medical applications. Curr Vasc Pharmacol 7:293–302

    Article  PubMed  PubMed Central  Google Scholar 

  • Maggiolini M et al (2005) The red wine phenolics piceatannol and myricetin act as agonists for estrogen receptor alpha in human breast cancer cells. J Mol Endocrinol 35:269–281

    Article  CAS  PubMed  Google Scholar 

  • Meijsing SH (2015) Mechanisms of glucocorticoid-regulated gene transcription. Adv Exp Med Biol 872:59–81

    Article  CAS  PubMed  Google Scholar 

  • Mullenders LHF (2018) Solar UV damage to cellular DNA: from mechanisms to biological effects. Photochem Photobiol Sci 17:1842–1852

    Article  CAS  PubMed  Google Scholar 

  • Ovesná Z et al (2006) Antioxidant activity of resveratrol, piceatannol and 3,3’,4,4’,5,5’-hexahydroxy-trans-stilbene in three leukemia cell lines. Oncol Rep 16:617–624

    PubMed  Google Scholar 

  • Puizina-Ivić N (2008) Skin aging. Acta Dermatovenerol Alp Pannonica Adriat 17:47–54

    PubMed  Google Scholar 

  • Schuch AP et al (2013) DNA damage as a biological sensor for environmental sunlight. Photochem Photobiol Sci 12:1259–1272

    Article  CAS  PubMed  Google Scholar 

  • Setoguchi Y et al (2014) Absorption and metabolism of piceatannol in rats. J Agric Food Chem 62:2541–2548

    Article  CAS  PubMed  Google Scholar 

  • Shiratake S et al (2015) Rose Myrtle (Rhodomyrtus Tomentosa) extract and its component, piceatannol, enhance the activity of DNA polymerase and suppress the inflammatory response elicited by UVB-induced DNA damage in skin cells. Mol Med Rep 12:5857–5864

    Article  CAS  PubMed  Google Scholar 

  • Sueishi Y, Nii R, Kakizaki N (2017) Resveratrol analogues like piceatannol are potent antioxidants as quantitatively demonstrated through the high scavenging ability against reactive oxygen species and methyl radical. Bioorg Med Chem Lett 27:5203–5206

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This paper was supported by Wonkwang University in 2021.

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Authors and Affiliations

  1. Division of Biological Sciences, Wonkwang University, Iksan, Republic of Korea

    Seula Jeong, Yuheon Chung, Sumin Lee, Nayoung Choi & Jong Kun Park

  2. Department of Food and Nutrition, Wonkwang University, Iksan, Republic of Korea

    Sojin Park

Authors
  1. Seula Jeong
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  2. Yuheon Chung
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  3. Sojin Park
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  4. Sumin Lee
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  5. Nayoung Choi
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  6. Jong Kun Park
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Contributions

SJ designed the research study, conducted experiments, acquired data, analyzed data and wrote the manuscript. YC, SP, SL and NC conducted experiments, acquiring data and analyzed data. JKP designed the research study, analyzed data, provided reagents, and wrote the manuscript.

Corresponding author

Correspondence to Jong Kun Park.

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Seula Jeong, Yuheon Chung, Sojin Park, Sumin Lee, Nayoung Choi and Jong Kun Park declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals by any of the authors.

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Jeong, S., Chung, Y., Park, S. et al. Combined treatment of ginsenoside Rg2 and piceatannol mixture reduces the apoptosis and DNA damage induced by UVB in HaCaT cells. Mol. Cell. Toxicol. 19, 63–70 (2023). https://doi.org/10.1007/s13273-022-00238-w

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  • DOI: https://doi.org/10.1007/s13273-022-00238-w

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