Abstract
This study evaluated in vivo anti-diabetic and anti-obesity activity of a polyherbal formulation’s methanolic extract containing an optimized ratio of edible seeds (Salvia hispanica, Chenopodium quinoa, Nelumbo nucifera). Diet-induced obese mice model (C57BL/6) was developed by feeding the mice a high-fat diet for 10 weeks resulting in hyperglycemia and obesity. Different doses (125, 250 and 500 mg/kg of body weight) of formulation were administered orally daily for 6 weeks. Fasting blood glucose and body weight were monitored throughout the study. At the end of the study, serum parameters were analyzed and histological examinations were performed. There was a significant reduction in fasting blood glucose levels and body weight in animal groups receiving polyherbal formulation. Lipid profile was improved as revealed by a reduction in serum triglycerides and total cholesterol. Histological study showed an improvement in liver, kidney and pancreatic sections of treated mice. High-performance thin layer chromatography was performed to identify the phytochemicals responsible for the above-mentioned bioactivities. The results revealed the presence of flavonoid (rutin) in seeds of N.nucifera and in the polyherbal formulation. For the first time, this study demonstrated the anti-diabetic and anti-obesity potential of the optimized formulation. The formulation can be used as a potential therapy for management of diabesity.
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Acknowledgements
This work was supported by JAIN (Deemed-to-be University), Bangalore, India. The authors sincerely thank the University Grants Commission for providing research grant to conduct the research. The authors are grateful to the Regional Ayurveda Research Institute for Metabolic Disorders, Bengaluru, India, for authentication of seed samples. The authors are also grateful to In Vivo Biosciences, Bengaluru, India, for providing the animal facility to carry out the experiment.
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University Grants Commission—South Eastern Regional Office, F1-17.1/2013-14/RGNF-2013-14-SC-BIH-36937, Tanisha.
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Tanisha performed the collection of the samples, extraction, designing and performing the experiment. SV guided the first author in performing animal study. MM designed the concept, corrections and drafting of the manuscript. The authors have read and approved this manuscript.
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Tanisha, Venkategowda, S. & Majumdar, M. Amelioration of hyperglycemia and hyperlipidemia in a high-fat diet-fed mice by supplementation of a developed optimized polyherbal formulation. 3 Biotech 12, 251 (2022). https://doi.org/10.1007/s13205-022-03309-w
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DOI: https://doi.org/10.1007/s13205-022-03309-w