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Influence of Caralluma adscendens Var. attenuata cold cream on UV-B damaged skin epidermal cells: a novel approach

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Abstract

Ultraviolet radiation-induced sunburns are characterized by pigmented, wrinkled, and dried skin, with rashes and red spots. Chemical sunscreen lotion shows beneficial effects, but it shows the adverse side effect while in continuous usage. Natural substances of plant origin are deemed a possible cause of UV radiation through sunscreen resources. On this basis, we formulated the cold cream from the Caralluma adscendens Var. attenuata (CAVA) plant extract. The phytocompounds were studied by using GC–MS. The antioxidant potential of the plant extract was determined, and the CAVA showed cytotoxicity on A375 skin melanoma cells determined by MTT assay. The FT-IR spectra analysis confirmed the chemical nature of crude and crosslinking between cold creams. The cream was applied topically to rats pre-exposed to UV-B radiation (32,800 J/m2) four times/week (on alternate days). UV-B exposed without any treatment rats showed increased red spots or wrinkles (5 cm2). In contrast, the cold cream treatment application on irradiated skin has significantly reduced the size of rashes and red spots and the wound was contracted in a dose-dependent manner. Furthermore, histopathology of the experimental rat skin confirmed that CAVA cream treatment significantly reduced the epidermal thickening, damage in dermis and epidermis layers, and restructured the hair follicles. This study suggests that the cream formulated using CAVA can alleviate the damages caused by the UV-B-irradiation at a high level and safeguard the skin tissues.

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Funding

The author’s research work by the self-financial scheme and no financial support received from any funding organizations.

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Authors and Affiliations

  1. Centre for Research and Postgraduate Studies in Botany, Ayya Nadar Janaki Ammal College, Sivakasi, Tamilnadu, 626124, India

    Madasamy Sundar & Krishnasamy Lingakumar

  2. Department of Botany, Directorate of Distance Education, Madurai Kamaraj University, Madurai, Tamilnadu, 625021, India

    Madasamy Sundar & Sudan Suresh

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  1. Madasamy Sundar
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  2. Sudan Suresh
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  3. Krishnasamy Lingakumar
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Contributions

The idea was conceived by KL and carried out by MS based on  the technical expertise of KL. The experimental part of the work and manuscript draft was duly done by MS. KL helped in the performed critical revision. Animal studies have been conducted by obtaining guidance from SS and KL. All authors read and approved the final manuscript.

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Correspondence to Krishnasamy Lingakumar.

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The authors state no conflict of interest.

Ethical approval and consent to participate

All procedures were performed following the National Institutes of Health Guidelines for the Care and Use of Laboratory Animals and were approved by the local animal protection committee.

Animal rights

The animal experiment was authorized according to the Institutions Committee on Animal Ethics (IAEC) of K.M College Pharmacy & MKU, Madurai, Tamilnadu, India (IAEC/SUNDAR.M/PhD/MKU/F9884/KMCP/70/2019). Totally 30 Animals were used for studies that are the basis of this research.

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Sundar, M., Suresh, S. & Lingakumar, K. Influence of Caralluma adscendens Var. attenuata cold cream on UV-B damaged skin epidermal cells: a novel approach. 3 Biotech 11, 155 (2021). https://doi.org/10.1007/s13205-021-02694-y

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