Abstract
Groundnut oil (GNO)/peanut oil is one of the agro-food products with great economic value and hence an attractive target for adulteration and mislabeling. Simple Sequence Repeats (SSR) are markers of choice for DNA fingerprinting studies as they exhibit high polymorphism due to variable number of repeats. Hence, this study was designed to evaluate and optimize a method for DNA isolation from groundnut oil and study the possibility of using the isolated DNA for molecular traceability using SSR markers. Four methods to isolate DNA from groundnut oil were evaluated. All the four methods were modified CTAB protocols, but differed in procedures for extraction, buffer compositions, amount of oil used and DNA carriers. For molecular traceability of oils, extraction and recovery of DNA from edible oil is a key step, especially in refined oils. A method that employed DNA enrichment prior to extraction with CTAB buffer yielded amplifiable DNA from cold pressed GNO, crude hexane extracted GNO and refined GNO. The optimized method for isolation of DNA from groundnut oil is simple, efficient, less costly and reproducible when compared to chromatography and spectroscopy based techniques.
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Entire data is available in the student academic repository and the laboratories where the experiment was conducted.
Abbreviations
- GNO:
-
Groundnut oil
- HCGNO:
-
Hexane extracted crude groundnut oil
- CPGNO:
-
Cold pressed groundnut oil
- CRGNO:
-
Commercially available refined groundnut oil
- SSR:
-
Simple Sequence Repeats
References
Arya SS, Salve AR, Chauhan S (2016) Peanuts as functional food: a review. J Food Sci Tech 53:31–41
Busconi M, Foroni C, Corradi M, Bongiorni C, Cattapan F, Fogher C (2003) DNA extraction from olive oil and its use in the identification of the production cultivar. Food Chem 83:127–134
Carrín ME, Carelli AA (2010) Peanut oil: compositional data. Eur J Lipid Sci Tech 112:697–707
Cheng X, Liu Z, Yan T, Wang Y, Yan L, Teng L, Wang F, Chen L, He Y, Guo K, Zhang D (2018) New method for effective identification of adulterated Camellia oil basing on Camellia oleifera-specific DNA. Arab J Chem 11:815–826
Consolandi C, Palmieri L, Severgnini M, Maestri E, Marmiroli N, Agrimonti C, Baldoni L, Donini P, Bellis G, Castiglioni B (2008) A procedure for olive oil traceability and authenticity: DNA extraction, multiplex PCR and LDR-universal array analysis. Eur Food Res Tech 227:1429–1438
Costa J, Mafra I, Amaral JS, Oliveira MBPP (2010) Monitoring genetically modified soybean along the industrial soybean oil extraction and refining processes by polymerase chain reaction techniques. Food Res Int 43:301–306
Costa J, Mafra I, Oliveira MBP (2012) Advances in vegetable oil authentication by DNA-based markers. Trends Food Sci Tech 26:43–55
Devasena N, Anitha BK, Manivannan N, Nallathambi G, Janila P, Pandey MK, Varshney RK (2017) Validation of SSR markers linked to oil content in groundnut (Arachis hypogaea L.). J Oilseeds Res 34:70–75
Dorni C, Sharma P, Saikia G, Longvah T (2018) Fatty acid profile of edible oils and fats consumed in India. Food Chem 238:9–15
Doveri S, Lee D (2007) Development of sensitive crop-specific polymerase chain reaction assays using 5S DNA: applications in food traceability. J Agri Food Chem 55:4640–4644
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Giménez MJ, Pistón F, Martín A, Atienza SG (2010) Application of real-time PCR on the development of molecular markers and to evaluate critical aspects for olive oil authentication. Food Chem 118:482–487
Gomez S, Martins-Lopes P, Guedes-Pinto H (2008) Olive tree genetic resources characterization through molecular markers. Genetic Diversity Plants 10:15–28
Gryson N, Ronsse F, Messens K, De Loose M, Verleyen T, Dewettinck K (2002) Detection of DNA during the refining of soybean oil. J Am Oil Chem Soc 79:171–174
He J, Xu W, Shang Y, Zhu P, Mei X, Tian W, Huang K (2013) Development and optimization of an efficient method to detect the authenticity of edible oils. Food Control 31:71–79
Herrero M, García Cañas V, Simo C, Cifuentes A (2010) Recent advances in the application of capillary electromigration methods for food analysis and foodomics. Electrophoresis 31:205–228
Li Y, Shao L, Fang X, Wan D, Wu Y, Li J, Zhu L (2018) Evaluation of five DNA extraction methods for commercial vegetable oils. Oil Crop Sci 3:122
List GR (2016) Peanut: genetics, processing, and utilization. Academic Press and AOCS Press, Washington. pp. 405–428 (processing and food uses of peanut oil and protein)
Mafra I, Ferreira IM, Oliveira MBP (2008) Food authentication by PCR-based methods. Eur Food Res Tech 227:649–665
Morello L, Braglia L, Gavazzi F, Gianì S, Breviario D (2019) Tubulin-based DNA barcode: principle and applications to complex food matrices. Genes (Basel) 10:229
Muzzalupo I, Pellegrino M, Perri E (2007) Detection of DNA in virgin olive oils extracted from destoned fruits. Eur Food Res Tech 224:469–475
Muzzalupo I, Pisani F, Greco F, Chiappetta A (2015) Direct DNA amplification from virgin olive oil for traceability and authenticity. Eur Food Res Tech 241:151–155
Nemati G, Shayan P, Kamkar A, Eckert B, Akhondzadeh BA, Noori N, Ashrafi T (2017) Analysis of DNA isolated from different oil sources: problems and solution. Iran J Vet Res 11:311–322
Nikolić Z, Vasiljević I, Zdjelar G, Đorđević V, Ignjatov M, Jovičić D, Milošević D (2014) Detection of genetically modified soybean in crude soybean oil. Food Chem 145:1072–1075
Ramos-Gómez S, Busto MD, Perez-Mateos M, Ortega N (2014) Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils. Food Chem 158:374–383
Shoba D, Manivannan N, Vindhiyavarman P, Nigam SN (2012) SSR markers associated for late leaf spot disease resistance by bulked segregant analysis in groundnut (Arachis hypogaea L.). Euphytica 188:265–272
Sujay V, Gowda MVC, Pandey MK (2012) Quantitative trait locus analysis and construction of consensus genetic map for foliar disease resistance based on two recombinant inbred line populations in cultivated groundnut (Arachis hypogaea L.). Mol Breed 30:773–788
Wu Y, Zhang H, Han J, Wang W, Ju X, Chen Y (2011) PCR-CE-SSCP applied to detect cheap oil blended in olive oil. Eur Food Res Tech 233:313–324
Yang R, Zhang L, Li P, Yu L, Mao J, Wang X, Zhang Q (2018) A review of chemical composition and nutritional properties of minor vegetable oils in China. Trends Food Sci Tech 74:26–32
Acknowledgments
The authors acknowledge the support of the Indian Council of Agricultural Research (ICAR) and MFPI—Quality Control Laboratory and Institute of Biotechnology of Professor Jayashankar Telangana State Agricultural University, Hyderabad for all the logistical assistance, infrastructure facility provided to carry out the study.
Funding
The research was conducted as a part of M.Sc Student Research work, supported by Professor Jayashankar Telangana State Agricultural University, Hyderabad, India.
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KPB: Conduct of Experiments, Investigation of results and validation of data, Writing the draft manuscript. AK: Conceptualization of research work and administration, Validation, Supervision of work, Writing review and editing. SNCVLP: Methodology, Investigation, Formal analysis, Validation, Supervision of work, Reviewing and editing of draft. SS: Methodology, Investigation, Proof reading and reviewing. MS: Methodology, Formal analysis, Review and editing of draft.
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Bojang, K.P., Kuna, A., Pushpavalli, S.N.C.V.L. et al. Evaluation of DNA extraction methods for molecular traceability in cold pressed, solvent extracted and refined groundnut oils. J Food Sci Technol 58, 3561–3567 (2021). https://doi.org/10.1007/s13197-021-05079-4
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DOI: https://doi.org/10.1007/s13197-021-05079-4