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Therapeutic effect of Linum usitatissimum L. in STZ-nicotinamide induced diabetic nephropathy via inhibition of AGE’s and oxidative stress

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Abstract

The present study was aimed to evaluate the potential of petroleum ether and hydro-alcoholic extract of Linum usitatissimum (FPE and FHE) in STZ-nicotinamide induced diabetic nephropathy. GC–MS analysis of FPE revealed the presence of different fatty acids, heterocyclic compounds etc. Moreover, chromatography of FHE isolated Secoisolariciresinol diglycoside. After 30 days of STZ-administration, 100, 200 and 400 mg/kg of FPE and FHE were administered for 45 days. FPE and FHE produced significant attenuation in the glycemic status, renal parameter, lipid profile and level of antioxidant enzymes proving efficacy in diabetic nephropathy. Moreover, FPE and FHE produced significant reduction in the formation of AGEs in kidney. The results indicated that via amelioration oxidative stress and formation of AGEs, FPE and FHE produced significant nephroprotective effect in STZ- induced diabetic nephropathy in rats.

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Abbreviations

ANOVA:

Analysis of variance

BUN:

Blood urea nitrogen

FHE:

Flaxseed hydroalcoholic extract

FPE:

Flaxseed petroleum ether extract

GSH:

Glutathione

HDL:

High density lipoproteins

LDL:

Low density lipoproteins

VLDL:

Very low density lipoproteins

i.p. :

Intraperitoneal administration

LPO:

Lipid peroxidation

MAPKs:

Mitogen activated protein kinases

SOD:

Superoxide dismutase

SDG:

Secoisolariciresinol diglycoside

STZ:

Streptozotocin

TBARS:

Thiobarbituric acid reactive substances

TNF-α:

Tumor necrosis factor-α

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Acknowledgements

Financial assistance (F. NO. SB/FT/LS-359/2012) from Department of Science and Technology, New Delhi, Government of India is highly acknowledged.

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  1. M.M. College of Pharmacy, M.M. University, Mullana-Ambala, Haryana, 133207, India

    Navpreet Kaur, Lalit Kishore & Randhir Singh

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  1. Navpreet Kaur
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  2. Lalit Kishore
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  3. Randhir Singh
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Correspondence to Randhir Singh.

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Kaur, N., Kishore, L. & Singh, R. Therapeutic effect of Linum usitatissimum L. in STZ-nicotinamide induced diabetic nephropathy via inhibition of AGE’s and oxidative stress. J Food Sci Technol 54, 408–421 (2017). https://doi.org/10.1007/s13197-016-2477-4

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  • DOI: https://doi.org/10.1007/s13197-016-2477-4

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