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Bile Salt Hydrolase (Bsh) Activity Screening of Lactobacilli: In Vitro Selection of Indigenous Lactobacillus Strains with Potential Bile Salt Hydrolysing and Cholesterol-Lowering Ability

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Abstract

The bile salt hydrolase (Bsh) activity of probiotic bacterium residing in gastrointestinal tract has often being associated with its cholesterol-lowering effects. Hence, Bsh activity was explored in this study as the criterion for the selection of most potential Bsh-active and cholesterol-lowering indigenous Lactobacillus strains. Forty lactobacilli were adjudged Bsh active after a preliminary screening of 102 lactobacilli and occurrence of Bsh activity correlated well with their natural habitats. Of the 40 shortlisted lactobacilli, fifteen putative Lactobacillus strains were selected and further tested for their comparative Bsh activity. In the end, indigenous Lactobacillus plantarum strains Lp91 and Lp21 were emerged as the promising Bsh-active lactobacilli with their substrate preference inclined more towards glycocholate than other bile acid amino conjugates. In addition, strains Lp91 and Lp21 also exhibited significantly high bile salt deconjugation, cholesterol assimilation and cholesterol co-precipitation ability in vitro. In conclusion, indigenous L. plantarum strains Lp91 and Lp21 may be the promising candidate probiotics to elucidate the ecological significance of probiotic Bsh activity in vivo.

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Acknowledgments

R. K. greatly appreciates the financial support received from Indian Council of Agricultural Research (ICAR, India) in terms of providing fellowship to work on his doctoral programme. Conceived, conceptualised and supervised the present study: R. K. S. G. and V. K. B; performed the experiments: R. K.; analysis and interpretation of data: R. K. S. G. and V. K. B.; contributed reagents/materials/analysis tools: S. G. and V. K. B.; preparation of the manuscript: R. K. S. G. and V. K. B. and have no conflict of interest.

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Authors and Affiliations

  1. Molecular Biology Unit, Dairy Microbiology Division, National Dairy Research Institute, Karnal, 132001, India

    Rajesh Kumar, Sunita Grover & Virender Kumar Batish

  2. Department of Microbiology and Immunology, National Institute of Nutrition, Hyderabad, 500007, India

    Rajesh Kumar

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  1. Rajesh Kumar
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  2. Sunita Grover
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  3. Virender Kumar Batish
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Correspondence to Rajesh Kumar or Virender Kumar Batish.

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Kumar, R., Grover, S. & Batish, V.K. Bile Salt Hydrolase (Bsh) Activity Screening of Lactobacilli: In Vitro Selection of Indigenous Lactobacillus Strains with Potential Bile Salt Hydrolysing and Cholesterol-Lowering Ability. Probiotics & Antimicro. Prot. 4, 162–172 (2012). https://doi.org/10.1007/s12602-012-9101-3

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