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Validation of the G1 Molecular Marker Associated with Resistance to Orange Rust in Brazilian Sugarcane Genotypes

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Abstract

Orange rust, caused by the fungus Puccinia kuehnii, is an important disease of sugarcane (Saccharum sp.). A molecular marker (G1) was developed to identify the resistance of sugarcane to orange rust. The aim of the present study was to validate the molecular marker G1 in the detection of resistance to orange rust in Brazilian sugarcane genotypes. 80 genotypes were analyzed by PCR, being classified according to the presence or absence of the marker. For field evaluation of orange rust resistance, a randomized block experiment was conducted, with the same 80 genotypes using four replications. Artificial inoculation was performed in the leaf whorl, with an evaluation made at 30 days after inoculation, using a scale from zero (without symptoms) to four (more than five pustules with sporulation), in which cultivars with an average score between zero and two were classified as resistant, and a score greater than two, susceptible. Of the 17 genotypes classified as resistant through the field test, 9 did not present the respective band. Of the 63 susceptible genotypes, 27 had the band relative to the molecular marker G1. The selection accuracy of G1 marker (the efficiency of predicting resistant phenotype) was 22.86%. The Phi correlation coefficient (ϕ) was calculated, through which it was observed that the variables are independent, with no statistically significant correlation between them. The results indicate that the G1 marker had a low efficiency to predict the resistance to orange rust in clones under selection in a Brazilian breeding program and Brazilian commercial cultivars of sugarcane.

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References

  • Alzate-Marin, A.L., G.D.L. Cervigni, M.A. Moreira, and E.G. Barros. 2005. Seleção assistida por marcadores moleculares visando ao desenvolvimento de plantas resistentes a doenças, com ênfase em feijoeiro e soja. Fitopatologia Brasileira 30: 333–342.

    Article  Google Scholar 

  • Amorim, L., A. Bergamin Filho, C. Cardoso, V.A. Moraes, and C.R. Fernandes. 1987. Metodologia de avaliação da ferrugem da cana de açúcar (Puccinia melanocephala). Boletim Técnico Copersucar 39: 13–16.

    Google Scholar 

  • Araújo, K.L., M.G. Canteri, T.A.S. Gilio, R.A. Neubauer, P.B. Sanches, C.H. Sumida, and É.A. Giglioti. 2013. Resistência genotípica e monitoramento da favorabilidade para ocorrência da ferrugem alaranjada da cana-de-açúcar. Summa Phytopathologica 39: 271–275.

    Article  Google Scholar 

  • Barreto, F.Z., T.W.A. Balsalobre, R.G. Chapola, H.P. Hoffmann, and M.S. Carneiro. 2017. Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar. Summa Phytopathologica 43: 36–40.

    Article  Google Scholar 

  • Chapola, R.G., H.P. Hoffmann, and N.S. Massola. 2016. Reaction of sugarcane varieties to orange rust (Puccinia kuehnii) and methods for rapid identification of resistant genotypes. Tropical Plant Pathology 41: 139–146.

    Article  Google Scholar 

  • Chelkowski, J., L. Golka, and L. Stepien. 2003. Application of STS markers for leaf rust resistance genes in near-isogenic lines of spring wheat cv. Thatcher. Journal of Applied Genetics 44: 323–338.

    PubMed  Google Scholar 

  • CONAB-Companhia Nacional de Abastecimento. Cana-de-açúcar, Safra 2019/2020. Terceiro levantamento, Dezembro de 2019. Disponível em https://www.conab.gov.br/info-agro/safras/cana/boletim-da-safra-de-cana-de-acucar.

  • Daros, E., R.A. Oliveira, J.L.C. Zambon, J.C. Bespalhok Filho, B.P. Brasileiro, O.T. Ido, L. Ruaro, and H. Weber. 2017. RB036088—A sugarcane cultivar for mechanical planting and harvesting. Crop Breeding and Applied Biotechnology 17: 84–88.

    Article  Google Scholar 

  • D’Hont, A., and J.C. Glaszmann. 2001. Sugarcane genome analysis with molecular markers, a first decade of research. Proceedings—International Society of Sugar Cane Technologists 24: 556–559.

    Google Scholar 

  • EMBRAPA. Sistema Brasileiro de Classificação de Solos. 2006. Rio de Janeiro: Embrapa Solos.

  • FAO. 2020. Brief Guide to Koeppen Climate Classification System. CLIMPAG: Global Climate Maps. http://www.fao.org/nr/climpag/climate/koeppen_en.htm. Acessed 17 April 2020.

  • FAOSTAT. 2020. Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/en/#data/QC. Acessed 09 Fev. 2020. Accessed 17 April 2020.

  • Ferreira, M.E., and D. Grattapaglia. 1995. Introdução ao uso de marcadores RAPD e RFLP em Análise Genética. Brasília: Embrapa, Cenargem.

    Google Scholar 

  • Fíer, I., T.W.A. Balsalobre, R.G. Chapola, H.P. Hoffmann, and M.S. Carneiro. 2020. Field resistance and molecular detection of the orange rust resistance gene linked to G1 marker in Brazilian cultivars of sugarcane. Summa Phytopathologica 46: 92–97.

    Article  Google Scholar 

  • Jena, K.K., and D.J. Mackill. 2008. Molecular markers and their use in marker-assisted selection in rice. Crop Science 48: 1266–1276.

    Article  Google Scholar 

  • Klosowski, A.C., J.C. Bespalhok Filho, L. Ruaro, and L.L. May de Mio. 2013. Inheritance of Resistance to Orange Rust (Puccinia kuehnii) in Sugarcane Families from Crosses Between Parents with Different Orange Rust Reactions. Sugar Tech 15 (4): 379–383.

    Article  Google Scholar 

  • Klosowski, A.C., J.C. Bespalhok Filho, L. Ruaro, R.B. Fragoso, and L.L. May de Mio. 2015. Reação de cultivares e época de avaliação da ferrugem alaranjada da cana-de-açúcar. Bioscience Journal 31: 489–498.

    Article  Google Scholar 

  • Lima, L.L.D., É.A.G. Scaloppi, L.F. Barreto, and M. Barreto. 2017. Temperaturas e períodos de molhamento foliar no desenvolvimento da ferrugem alaranjada da cana-de-açúcar (Puccinia kuehnii). Summa Phytopathologica 23: 132–135.

    Article  Google Scholar 

  • Lira, S.A., and A. Chaves Neto. 2006. Coeficientes de correlação para variáveis ordinais e dicotômicas derivados do coeficiente linear de Pearson. Ciência & Engenharia 15 (1/2): 45–53.

    Google Scholar 

  • Matsuoka, S., J.A. Ferro, and P. Arruda. 2009. The Brazilian experience of sugarcane ethanol industry. Vitro Cellular and Developmental Biology: Plant 45: 372–381.

    Article  Google Scholar 

  • McCord, P., N. Glynn, and J. Comstock. 2019. Identifying markers for resistance to sugarcane orange rust (Puccinia kuehnii) via selective genotyping and capture sequencing. Euphytica 215: 150.

    Article  Google Scholar 

  • McIntyre, C.L., V.A. Whan, B. Croft, R. Magarey, and G.R. Smith. 2005. Identification and validation of molecular markers associated with Pachymetra root rot and brown rust resistance in sugarcane using map- and association-based approaches. Molecular Breeding 16: 151–161.

    Article  CAS  Google Scholar 

  • Minchio, C.A., M.G. Canteri, and J.A. Rocha. 2011. Germinação de uredósporos de Puccinia kuehnii submetidos a diferentes temperaturas e tempos de incubação. Summa Phytopathologica 37: 211–214.

    Article  Google Scholar 

  • Nitsche, P.R., P.H. Caramori, W.S. Ricce, and L.F.D. Pinto. 2019. Atlas Climático do Estado do Paraná. Londrina (PR): Instituto Agronômico do Paraná.

    Google Scholar 

  • Pathania, A., N. Rialch and P.N. Sharma. 2016. Marker-Assisted Selection in Disease Resistance Breeding: A Boon to Enhance Agriculture Production. In Current Developments in Biotechnology and Bioengineering, United States, 1st ed., 187–213. Amsterdam, The Netherlands.

  • Silva, P.R., S.C.K. Milach, V.A. Sortica, T. Boff, S.P. Brammer, and L.C. Federizzi. 2008. Validação de marcadores moleculares associados a genes de resistência à ferrugem-da-folha-do-trigo. Pesquisa Agropecuária Brasileira 43: 1357–1363.

    Article  Google Scholar 

  • Sood, S.G., J.C. Comstock, and N.C. Glynn. 2009. Leaf whorl inoculation method for screening sugarcane rust resistance. Plant Disease 93: 1335–1340.

    Article  Google Scholar 

  • Sood, S.G., J.C. Comstock, and R.N. Raid. 2013. Evaluation of sugarcane clones in the CP-cultivar program for resistance to Puccinia kuehnii, the pathogen of orange rust. Proceedings International Society of Sugar Cane Technologists 28: 1–7.

    Google Scholar 

  • Tokeshi, H. and A. Rago. 2005. Doenças da cana-de-açúcar (híbridos de Saccharum spp.). In Manual de Fitopatologia: doenças das plantas cultivadas, Volume 2, 185–196. São Paulo: Ceres.

  • Urbanovich, O.Y., S.V. Malyshev, T.V. Dolmatovich, and N.A. Kartel. 2006. Identification of leaf rust resistance genes in wheat (Triticum aestivum L.) cultivars using molecular markers. Russian Journal of Genetics 42: 546–554.

    Article  CAS  Google Scholar 

  • Yang, X., M.S. Islam, S. Sood, S. Maya, E.A. Hanson, J. Comstock, and J. Wang. 2018a. Identifying quantitative trait loci (QTLs) and developing diagnostic markers linked to orange rust resistance in sugarcane (Saccharum spp.). Frontiers in Plant Science 9: 350.

    Article  Google Scholar 

  • Yang, X., S. Sood, Z. Luo, J. Todd, and J. Wang. 2018b. Genome-wide association studies identified resistance loci to orange rust and yellow leaf virus diseases in sugarcane (Saccharum spp.). Phytopathology 109: 623–631.

    Article  Google Scholar 

  • Zhao, D., N.C. Glynn, B. Glaz, J.C. Comstock, and S. Sood. 2011. Orange rust effects on leaf photosynthesis and related characters of sugarcane. Plant Disease 95: 640–647.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financed in part by the Coordination of Superior Level Staff Improvement (CAPES/Brazil) – Finance Code 001 and by the ‘Inter-University Network for the Development of the Sugarcane Industry (RIDESA/PMGCA/UFPR).’ HSSD received a research fellowship from National Council for Scientific and Technological Development (CNPq/Brazil).

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Authors and Affiliations

  1. Departamento de Fitotecnia e Fitossanidade, Programa de Pós-graduação em Agronomia, Universidade Federal do Paraná, Rua dos Funcionários, 1540, Cabral, Curitiba, Paraná, CEP 80035-050, Brazil

    Lívia Maria Lemos Hoepers, Gabriel Koch, Mayara Catherine Candido Silva, Lucimeris Ruaro, Renata Faier Calegario, João Carlos Bespalhok Filho, Ricardo Augusto de Oliveira & Henrique da Silva Silveira Duarte

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  1. Lívia Maria Lemos Hoepers
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  2. Gabriel Koch
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  3. Mayara Catherine Candido Silva
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  4. Lucimeris Ruaro
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  5. Renata Faier Calegario
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  6. João Carlos Bespalhok Filho
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  7. Ricardo Augusto de Oliveira
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  8. Henrique da Silva Silveira Duarte
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Correspondence to Henrique da Silva Silveira Duarte.

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Hoepers, L.M.L., Koch, G., Silva, M.C.C. et al. Validation of the G1 Molecular Marker Associated with Resistance to Orange Rust in Brazilian Sugarcane Genotypes. Sugar Tech 23, 108–117 (2021). https://doi.org/10.1007/s12355-020-00896-x

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