Abstract
In higher plants, sucrose synthase (Susy, EC 2.4.1.13) as an enzyme with a core function, involved in the synthesis and breakdown of sugars, and plays an important role in growth and metabolism. Although, the different genes encoding Susy isozyme proteins have been cloned and functionally verified in several plant species, to date detailed information about the Susy genes is lacking in Sorghum. Here, we demonstrated the identification of five novel Susy genes from the sorghum genome database. Sequence, structure and phylogenetic analyses of these five SbSusy genes revealed evolutionary conservation through Susy gene family members across Sorghum and other crop plants. The expression of sorghum Susy genes was investigated via transcriptome database in various developmental stages and different tissues. Further qRT-PCR was performed to reveal the induction of SbSusy genes under salt, drought and sugar induction. The results indicated that all Susy genes were differentially expressed in various tissues and highly associated with sucrose metabolism. This study shows a theoretical reference of Susy genes in Sorghum, which provides new insights for the knowledge of the evolution relationships, and basic information to help clarify the molecular mechanism of Susy synthase genes in Sorghum.
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Acknowledgements
The authors thank the Dr. Jiwei Zhang from Sichuan Agricultural University for providing Btx623 seeds for abiotic stress treatment.
Funding
This work was supported by the Research Initiation Fund for High-level Talents of China Three Gorges University (2016GCRC02).
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Shaopeng Han: Investigation, Methodology, Writing-original draft. Yelei Lu: Writing, Resources, Data Curation. Chao Zhou: Conceptualization, Supervision. Yunwei Cheng: Resources, Data Curation. Yang Lv: Resources, Data Curation. Gongjian Zeng: Resources. Dechun Zhang: Writing-review & editing. Xinqiang Gao: Writing-review & editing. Xiangling Shen: Conceptualization, Supervision, Writing-review & editing. All authors read and approved the final manuscript.
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12298_2022_1166_MOESM1_ESM.tif
Supplement Figure 1. Identity matrix for SbSusy amino acid sequences. Colors from red to blue indicate high to low expressions. (TIF 1247 kb)
12298_2022_1166_MOESM2_ESM.tif
Supplementary Figure 2. Distribution of conserved motifs for SbSusy genes. Conserved motifs are marked by different colors on the right. (TIF 1639 kb)
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Lu, Y., Han, S., Zhou, C. et al. Molecular identification and expression analysis of five sucrose synthase genes in Sorghum Bicolor. Physiol Mol Biol Plants 28, 697–707 (2022). https://doi.org/10.1007/s12298-022-01166-8
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DOI: https://doi.org/10.1007/s12298-022-01166-8