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In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Abstract

The administration time is a critical but long-neglected point in cell therapy based on macrophages because the incorrect time of macrophage administration could result in diverse outcomes regarding the same macrophage therapy. In this work, the second near-infrared (NIR-II) fluorescence imaging in vivo tracking of M2 macrophages during a pro-healing therapy in the mice model of rotator cuff injury revealed that the behavior of administrated macrophages was influenced by the timing of their administration. The delayed cell therapy (DCT) group had a longer retention time of injected M2 macrophages in the repairing tissue than that in the immediate cell therapy (ICT) group. Both Keller–Segel model and histological analysis further demonstrated that DCT altered the chemotaxis of M2 macrophages and improved the healing outcome of the repaired structure in comparison with ICT. Our results offer a possible explanation of previous conflicting results on reparative cell therapy and provoke reconsideration of the timing of these therapies.

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Acknowledgements

This animal research received the approval of ethics by Ethics Committee of Fudan University (No. 202208005Z). This work was supported by the National Natural Science Foundation of China (Nos. 81972129, 82072521, and 82111530200), Shanghai Talent Development Funding Scheme (No. 2020080), and Shanghai Committee of Science and Technology (Nos. 22DZ2204900 and 23ZR1445700). J. Chen and N. T. K. Thanh thank the Royal Society and National Natural Science Foundation of China for the International Exchanges program for funding. The authors thank Hao Chen and his team from Shanghai Institute of Materia Medica, Chinese Academy of Sciences for providing the NIR-II fluorescence imaging instrument.

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Author notes

  1. Yuzhou Chen, Mo Chen, and Chengxuan Yu contributed equally to this work.

Authors and Affiliations

  1. Sports Medicine Institute of Fudan University, Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai, 200040, China

    Yuzhou Chen, Mo Chen, Chengxuan Yu, Huizhu Li, Jian Zhang, Shiyi Chen, Yunxia Li, Sijia Feng & Jun Chen

  2. Department of Orthopedic Surgery, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, 200092, China

    Yuzhou Chen

  3. Department of Bone and Joint Surgery, Department of Orthopedics, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200001, China

    Mo Chen

  4. Department of Physics, Shanghai Normal University, Shanghai, 200234, China

    Liman Sai

  5. Biophysics Group, Department of Physics and Astronomy, University College London, London, WC1E 6BT, UK

    Nguyen T. K. Thanh

  6. UCL Healthcare Biomagnetic and Nanomaterials Laboratories, London, W1S 4BS, UK

    Nguyen T. K. Thanh

  7. Department of Anatomy and Physiology, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200025, China

    Yueming Wang & Yan Wo

  8. Department of Orthopedics, Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou, 215500, China

    Xing Yang & Yuefeng Hao

  9. Institute of Biology and Biomedicine, Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod, 603950, Russia

    Evgenii L. Guryev & Andrei V. Zvyagin

  10. Mathematical Institute, University of Oxford, Oxford, OX2 6GG, UK

    Hao De

  11. Institute of Natural Sciences, School of Mathematics, Shanghai Jiao Tong University, Shanghai, 200240, China

    Min Tang

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  1. Yuzhou Chen
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  2. Mo Chen
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Correspondence to Yunxia Li, Yuefeng Hao, Sijia Feng or Jun Chen.

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In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging

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Chen, Y., Chen, M., Yu, C. et al. In vivo real-time monitoring delayed administration of M2 macrophages to enhance healing of tendon by NIR-II fluorescence imaging. Nano Res. 17, 4379–4390 (2024). https://doi.org/10.1007/s12274-023-6363-x

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  • DOI: https://doi.org/10.1007/s12274-023-6363-x

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