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Study on a Low-temperature Cellulose-degrading Strain: Fermentation Optimization, Straw Degradation, and the Effect of Fermentation Broth on Seed Growth

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Abstract

The biodegradation of straw by cellulose-degrading strains plays an important role in the disposal of agricultural waste. In this study, a Penicillium sp. strain JiTF01 that could degrade cellulose was isolated at the low temperature (10°C), and its fermentation conditions were optimized by response surface method. Strain JiTF01 was applied to degrade rice straw at 10°C. It was found that the straw degradation ratio could reach to 46.53% on the 25th day of culture with the enzyme activity of 44.30 U/mL under optimized fermentation conditions. In addition, given that strain JiTF01 showed excellent acid resistance and the cellulase produced by strain JiTF01 exhibited higher enzymatic activity and stability under acidic conditions, the uninterrupted integration process of acid pretreatment and acid fermentation of straw was then performed. The results showed that the degradation ratio of 53.61% was observed after 25 days of the fermentation at pH 3, which increased by 7.08% than that under optimized conditions. Besides, the effect of straw fermentation broth on the growth of rice seed was also investigated, and the 10−5 and 10−6 diluted fermentation broth was found that could significantly promote the growth of rice seedlings and enhance the germination rate of rice seeds under the salt stress of 100 and 200 mmol/L. The conclusions found in this article might have important implications for straw recycling in cold regions.

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Acknowledgements

This work was supported by Tianjin Science and Technology Research and Development Plan Project (Grant numbers: 19YFZCSN00280) and Tianjin Rice Industry Technology System Innovation Team Construction (Grant numbers: ITTRRS2018007).

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Authors and Affiliations

  1. School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China

    Xueli Dong, Jing Ji, Songhao Zhang, Danliu Peng, Lishuang Zhang, Jiali Li & Gang Wang

  2. Tianda Tianfu Biotechnology Co., Ltd., Tianjin, 300384, China

    Yurong Wang

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  1. Xueli Dong
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Dong, X., Ji, J., Zhang, S. et al. Study on a Low-temperature Cellulose-degrading Strain: Fermentation Optimization, Straw Degradation, and the Effect of Fermentation Broth on Seed Growth. Biotechnol Bioproc E 27, 652–667 (2022). https://doi.org/10.1007/s12257-021-0265-0

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