Skip to main content
SpringerLink
Log in

Cellulase with high β-glucosidase activity by Penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue

  • Original Paper
  • Published:
World Journal of Microbiology and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, we investigated cellulase production by Penicillium oxalicum EU2106 under solid-state fermentation (SSF) and its hydrolysis efficiency toward NaOH–H2O2-pretreated cassava residue (NHCR) produced after bioethanol fermentation. Optimization of SSF cultivation conditions for P. oxalicum EU2106 using a Box-behnken design-based response-surface methodology resulted in maximal cellulase activity of 34.0 ± 2.8 filter-paper units/g dry substrate, exhibiting a ~ twofold increase relative to activities obtained under non-optimized conditions. Furthermore, SSF-derived cellulase converted 94.3 ± 1.5% of NHCR cellulose into glucose within 96 h. Interestingly, P. oxalicum EU2106 produced higher β-glucosidase activity under SSF conditions than that under submerged-state fermentation conditions, resulting in the elimination of cellobiose inhibition during the early stages of NHCR cellulose hydrolysis. Overall, this work provided an alternative for a potential cellulase source and a preferred option for cassava residue biotechnological application.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

BBD:

Box-behnken design

BGL:

β-glucosidase

CB:

Corncob

CGMCC:

The China general microbiological culture collection center

CBH:

Cellobiohydrolase

CMCase:

Carboxymethylcellulose cellulase

CR:

Cassava residue

EG:

Endo-1,4-β-d-glucanase

FPase:

Filter-paper cellulase

FPU:

Filter-paper units

Gds:

Gram of dry substrate

HPLC:

High-performance liquid chromatography

NHCR:

NaOH–H2O2 pretreated cassava residue

pNPCase:

p-nitrophenyl-β-cellobioside cellulase

pNPGase:

p-nitrophenyl-β-glucopyranoside cellulase

RH:

Rice husk

RS:

Rice straw

RSM:

Response-surface methodology

SSF:

Solid-state fermentation

SmF:

Submerged-state fermentation

SB:

Sugarcane bagasse

WB:

Wheat bran

References

  • Bansal N, Tewari R, Soni R, Soni SK (2012) Production of cellulases from Aspergillus niger NS-2 in solid state fermentation on agricultural and kitchen waste residues. Waste Manag 32:1341–1346

    Article  CAS  Google Scholar 

  • Brijwani K, Oberoi HS, Vadlani PV (2010) Production of a cellulolytic enzyme system in mixed-culture solid-state fermentation of soybean hulls supplemented with wheat bran. Process Biochem 45:120–128

    Article  CAS  Google Scholar 

  • Camassola M, Dillon AJ (2010) Cellulases and xylanases production by Penicillium echinulatum grown on sugar cane bagasse in solid-state fermentation. Appl Biochem Biotech 162:1889–1900

    CAS  Google Scholar 

  • Delabona PD, Pirota R, Codima CA, Tremacoldi CR, Rodrigues A, Farinas CS (2013) Effect of initial moisture content on two Amazon rainforest Aspergillus strains cultivated on agro-industrial residues: biomass-degrading enzymes production and characterization. Ind Crops Prod 42:236–242

    Article  CAS  Google Scholar 

  • Deswal D, Khasa YP, Kuhad RC (2011) Optimization of cellulase production by a brown rot fungus Fomitopsis sp. RCK2010 under solid state fermentation. Bioresour Technol 102:6065–6072

    Article  CAS  Google Scholar 

  • Dhillon GS, Oberoi HS, Kaur S, Bansal S, Brar SK (2011) Value-addition of agricultural wastes for augmented cellulase and xylanase production through solid-state tray fermentation employing mixed-culture of fungi. Ind Crop Prod 34: 1160–1167.

    Article  CAS  Google Scholar 

  • El-Shishtawy RM, Mohamed SA, Asiri AM, Gomaa ABM, Ibrahim IH, Al-Talhi HA (2015) Saccharification and hydrolytic enzyme production of alkali pre-treated wheat bran by Trichoderma virens under solid state fermentation. BMC Biotechnol 15:1–13

    Article  CAS  Google Scholar 

  • Farinas CS (2015) Development in solid-state fermentation for the production of biomass-degrading enzymes for the bioenergy sector. Renew Sust Energ Rev 52:179–188

    Article  CAS  Google Scholar 

  • Garrote G, Yanez R, Alonso JL, Parajo JC (2008) Coproduction of oligosaccharides and glucose from corncobs by hydrothermal processing and enzymatic hydrolysis. Ind Eng Chem Res 47:1336–1345

    Article  CAS  Google Scholar 

  • Gusakov AV (2011) Alternatives to Trichoderma reesei in biofuel production. Trends Biotechnol 29:419–425

    Article  CAS  Google Scholar 

  • Huang Y, Qin X, Luo XM, Nong QD, Yang Q, Zhang Z, Gao Y, Lu FX, Chen Y, Yu ZW, Liu JL, Feng JX (2015) Efficient enzymatic hydrolysis and simultaneous saccharification and fermentation of sugarcane bagasse pulp for ethanol production by cellulase from Penicillium oxalicum EU2106 and thermotolerant Saccharomyces cerevisiae ZM1-5. Biomass Bioenerg 77: 53–63.

    Article  CAS  Google Scholar 

  • Jing L, Zhao S, Xue JL, Zhang Z, Yang Q, Xian L, Feng JX (2015) Isolation and characterization of a novel Penicillium oxalicum strain Z1-3 with enhanced cellobiohydrolase production using cellulase-hydrolyzed sugarcane bagasse as carbon source. Ind Crop Prod 77: 666–675.

    Article  CAS  Google Scholar 

  • Latifian M, Hamidi-Esfahani Z, Barzegar M (2007) Evaluation of culture conditions for cellulase production by two Trichoderma reesei mutants under solid-state fermentation conditions. Bioresour Technol 98:3634–3637

    Article  CAS  Google Scholar 

  • Martínez PM, Bakker R, Harmsen P, Gruppen H, Kabel M (2015) Importance of acid or alkali concentration on the removal of xylan and lignin for enzymatic cellulose hydrolysis. Ind Crop Prod 64: 88–96.

    Article  Google Scholar 

  • Matkar K, Chapla D, Divecha J, Nighojkar A, Madamwar D (2013) Production of cellulase by a newly isolated strain of Aspergillus sydowii and its optimization under submerged fermentation. Int Biodeter Biodegr 78: 24–33.

    Article  CAS  Google Scholar 

  • Mo H, Zhang X, Li Z (2004) Control of gas phase for enhanced cellulase production by Penicillium decumbens in solid-state culture. Process Biochem 39:1293–1297

    Article  CAS  Google Scholar 

  • Rabemanolontsoa H, Saka S (2016) Various pretreatments of lignocellulosics. Bioresour Technol 199:83–91

    Article  CAS  Google Scholar 

  • Raghuwanshi S, Deswal D, Karp M, Kuhad RC (2014) Bioprocessing of enhanced cellulase production from a mutant of Trichoderma asperellum RCK2011 and its application in hydrolysis of cellulose. Fuel 124:183–189

    Article  CAS  Google Scholar 

  • Saini R, Saini JK, Adsul M, Patel AK, Mathur A, Tuli D, Singhania RR (2015) Enhanced cellulase production by Penicillium oxalicum for bio-ethanol application. Bioresour Technol 188:240–246

    Article  CAS  Google Scholar 

  • Sherief AA, El-Tanash AB, Atia N (2010) Cellulase production by Aspergillus fumigatus grown on mixed substrate of rice straw and wheat Bran. Res. J Microbiol 5:199–211

    CAS  Google Scholar 

  • Sluiter A, Hames B, Ruiz R, Scarlata C, Sluiter J, Templeton D, Crocker D (2012) Determination of structural carbohydrates and lignin in biomass. Technical. Report NREL/TP-510-42618-revised 2012.

  • Sun XY, Liu ZY, Qu YB, Li XZ (2008) The effects of wheat bran composition on the production of biomass-hydrolyzing enzymes by Penicillium decumbens. Appl Biochem Biotechnol 1:119–128

    Article  Google Scholar 

  • Wang JP, Quirk A, Lipkowski J, Dutcher JR, Clarke AJ (2013a) Direct in situ observation of synergism between cellulolytic enzymes during the biodegradation of crystalline cellulose fibers. Langmuir 29:14997–15005

  • Wang W, Luo G, Xie L, Zhou Q (2013b) Enhanced thermophilic fermentative hydrogen production from cassava stillage by chemical pretreatments. Water Sci Technol 68:59–67

  • Xu XQ, Wu XB, Cui Y, Cai YX, Liu RW, Long MN, Chen QX (2014) Enzymatic saccharification of cassava residues and glucose inhibitory kinetics on β-glucosidase from Hypocrea orientalis. J Agr Food Chem 62:11512–11518

    Article  CAS  Google Scholar 

  • Xue JL, Zhao S, Liang RM, Yin X, Jiang SX, Su LH, Yang Q, Duan CJ, Liu JL, Feng JX (2016) A biotechnological process efficiently co-produces two high value-added products, glucose and xylooligosaccharides, from sugarcane bagasse. Bioresour Technol 204:130–138

    Article  CAS  Google Scholar 

  • Zhang Q, Tang L, Zhang J, Mao Z, Jiang L (2011) Optimization of thermal-dilute sulfuric acid pretreatment for enhancement of methane production from cassava residues. Bioresour Technol 102:3958–3965

    Article  CAS  Google Scholar 

  • Zhang M, Xie L, Yin ZX, Khanal SK, Zhou Q (2016) Biorefinery approach for cassava-based industrial wastes: current status and opportunities. Bioresour Technol 215:50–62

    Article  CAS  Google Scholar 

  • Zhao S, Yan YS, He QP, Yang L, Yin X, Li CX, Mao LC, Liao LS, Huang JQ, Xie SB, Nong QD, Zhang Z, Jing L, Xiong YR, Duan CJ, Liu JL, Feng JX (2016) Comparative genomic, transcriptomic and secretomic profiling of Penicillium oxalicum HP7-1 and its cellulase and xylanase hyper-producing mutant EU2106, and identification of two novel regulatory genes of cellulase and xylanase gene expression. Biotechnol Biofuel 9: 203.

    Article  Google Scholar 

Download references

Acknowledgements

This work was financially supported by grants from the Guangxi BaGui Scholars Program Foundation (Grant Number 2011A001), the ‘One Hundred Person’ Project of Guangxi, the National Natural Science Foundation of China (Grant Number 31260017 and 31660305), and Excellent Teaching Program of Guangxi High Education– Program of Advantageous and Characteristic Specialty (Excellent Undergraduate Major).

Author information

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University, 100 Daxue Road, Nanning, 530004, Guangxi, People’s Republic of China

    Lin-Hui Su, Shuai Zhao, Sui-Xin Jiang, Xu-Zhong Liao, Cheng-Jie Duan & Jia-Xun Feng

Authors
  1. Lin-Hui Su
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shuai Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sui-Xin Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xu-Zhong Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Cheng-Jie Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jia-Xun Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jia-Xun Feng.

Additional information

Lin-Hui Su and Shuai Zhao have contributed equally to this work.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (PDF 823 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Su, LH., Zhao, S., Jiang, SX. et al. Cellulase with high β-glucosidase activity by Penicillium oxalicum under solid state fermentation and its use in hydrolysis of cassava residue. World J Microbiol Biotechnol 33, 37 (2017). https://doi.org/10.1007/s11274-016-2200-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11274-016-2200-7

Keywords

Search

Navigation