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Evaluation of probiotic bifidobacteria strains from Iranian traditional dairy products for their anti-hyperlipidemic potential

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Abstract

This study investigated the therapeutic potential of probiotic bifidobacteria, isolated from Iranian fermented dairy products, in a hyperlipidemic animal model. Bifidobacterium strains were extracted from traditional dairy samples and screened using physiological and phenotypic examinations, 16S rRNA analysis, and probiotic properties such as tolerance to gastrointestinal juice, antimicrobial activity, and antibiotic susceptibility. The ability of the screened bifidobacteria to reduce serum and liver lipids in vivo was tested using male Wistar rats. Six strains of bifidobacteria were isolated from traditional Iranian fermented dairy. These strains showed promising in vitro activity in lowering triglyceride and cholesterol, tolerance to simulated gastrointestinal juice, the ability to adhere to Caco-2 cells, acceptable antibiotic susceptibility, and a broad spectrum of antibacterial activity. The diet supplemented with isolated bifidobacteria significantly reduced serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), liver tissue lipid levels, and hepatic enzymes in animals when compared to a high-fat diet without strains (p < 0.01). Additionally, the potential probiotic-supplemented diet significantly increased bile acid excretion in the feces and upregulated hepatic CYP7A1 expression levels (p < 0.05), while NPC1L1, ACAT2, and MTP gene expressions in small intestinal cells were downregulated (p < 0.05). Bifidobacteria isolated from Iranian traditional dairy showed potential for use in the production of fermented foods that have hypolipemic activity in the host.

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Data availability

The authors declare that the data supporting the findings of this study are available within the paper and its Supplementary Information files. Should any raw data files be needed in another format they are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Sciences, Arak Branch, Islamic Azad University, Arak, Iran

    Nasim Afshar

  2. Department of Microbiology, Faculty of Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran

    Kumarss Amini

  3. Department of Biology, Arak Branch, Islamic Azad University, Arak, Iran

    Hamidreza Mohajerani

  4. Department of Medical Laboratory Sciences, Faculty of Medical Sciences, Arak Branch, Islamic Azad University, Arak, Iran

    Sasan Saki

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  1. Nasim Afshar
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  2. Kumarss Amini
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  3. Hamidreza Mohajerani
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  4. Sasan Saki
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Correspondence to Kumarss Amini.

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All applicable international guidelines for the care and use of animals were followed. Animal studies in this experiment were carried out strictly according to the rules of the Animal Welfare and Research Ethics Committee of Islamic Azad University (Arak, Iran), and the permit number was IR.IAU.ARAK.REC.1399.018.

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Afshar, N., Amini, K., Mohajerani, H. et al. Evaluation of probiotic bifidobacteria strains from Iranian traditional dairy products for their anti-hyperlipidemic potential. Folia Microbiol (2024). https://doi.org/10.1007/s12223-023-01124-1

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