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From Efficiency to Yield: Exploring Recent Advances in CHO Cell Line Development for Monoclonal Antibodies

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Abstract

The increasing demand for biosimilar monoclonal antibodies (mAbs) has prompted the development of stable high-producing cell lines while simultaneously decreasing the time required for screening. Existing platforms have proven inefficient, resulting in inconsistencies in yields, growth characteristics, and quality features in the final mAb products. Selecting a suitable expression host, designing an effective gene expression system, developing a streamlined cell line generation approach, optimizing culture conditions, and defining scaling-up and purification strategies are all critical steps in the production of recombinant proteins, particularly monoclonal antibodies, in mammalian cells. As a result, an active area of study is dedicated to expression and optimizing recombinant protein production. This review explores recent breakthroughs and approaches targeted at accelerating cell line development to attain efficiency and consistency in the synthesis of therapeutic proteins, specifically monoclonal antibodies. The primary goal is to bridge the gap between rising demand and consistent, high-quality mAb production, thereby benefiting the healthcare and pharmaceutical industries.

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Acknowledgements

SM thanks University Grant Commission (UGC) for Savitribai Jyotirao Phule Single Girl Child fellowship; Registration ID: UGC-22-GE-MAH-F-SJSGC-10886, RD thanks Council of Scientific and Industrial Research (CSIR); NO: 09/991(0059)/2019-EMR-I for doctoral fellowship.

Funding

University Grant Commission (UGC), Council of Scientific and Industrial Research (CSIR), Department of Science and Technology (DST), Science and Engineering Research Board (SERB), Department of Biotechnology (DBT).

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Authors and Affiliations

  1. Department of Biological Science and Biotechnology, Institute of Chemical Technology, Mumbai, 400019, India

    Sarmishta Majumdar & Ratnesh Jain

  2. Department of Pharmaceutical Sciences and Technology, Institute of Chemical Technology, Mumbai, 400019, India

    Ranjeet Desai, Aakarsh Hans & Prajakta Dandekar

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  1. Sarmishta Majumdar
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  2. Ranjeet Desai
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SM, RJ, & PD contributed to manuscript conception and preparation; SM, RD, & AH contributed to manuscript drafting and revision; RJ & PD contributed to proofreading and manuscript revision.

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Correspondence to Prajakta Dandekar or Ratnesh Jain.

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Majumdar, S., Desai, R., Hans, A. et al. From Efficiency to Yield: Exploring Recent Advances in CHO Cell Line Development for Monoclonal Antibodies. Mol Biotechnol (2024). https://doi.org/10.1007/s12033-024-01060-6

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