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Artificial Transcription Factors Increase Production of Recombinant Antibodies in Chinese Hamster Ovary Cells

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Abstract

A randomized library that encodes for artificial zinc finger protein transcription factors (ZFP-TF) was constructed and screened for components that increased production of a monoclonal antibody (mAb-72) in Chinese hamster ovary (CHO) cells. One of these ZFP-TF, LK52, increased mAb-72 production ~10-fold at ~60% transduction efficiency; a mutated version of LK52, however, did not boost mAb-72 production. LK52 also increased production of other mAbs in CHO cells. These results demonstrate that ZFP-TF libraries can be used to identify components that improve antibody production in CHO cells.

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Authors and Affiliations

  1. ToolGen, Inc. Daejeon Bio-Venture Town, 461-58, Jeonmin-dong, 305-390, Yuseong-gu, Daejeon, South Korea

    Ryuk-Jun Kwon, Seong Keun Kim, Seung-Il Lee, Jin-Soo Kim & Wongi Seol

  2. Department of Biological Sciences, Korea Advanced Institute of Science and Technology, 373-1, Kusung-dong, 305-701, Yuseong-gu, Daejeon, South Korea

    Su-Jeong Hwang & Gyun Min Lee

  3. Department of Chemistry, Seoul National University, Shinrim-dong, 151-742, Kwanak-gu, Seoul, South Korea

    Jin-Soo Kim

  4. Institute for Brain Science and Technology, Inje University, Hanwha CC building, 6 Shinsung-dong, 305-804, Yuseong-gu, Daejeon, South Korea

    Wongi Seol

Authors
  1. Ryuk-Jun Kwon
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  2. Seong Keun Kim
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  3. Seung-Il Lee
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  4. Su-Jeong Hwang
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  5. Gyun Min Lee
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  6. Jin-Soo Kim
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  7. Wongi Seol
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Correspondence to Wongi Seol.

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Kwon, RJ., Kim, S.K., Lee, SI. et al. Artificial Transcription Factors Increase Production of Recombinant Antibodies in Chinese Hamster Ovary Cells. Biotechnol Lett 28, 9–15 (2006). https://doi.org/10.1007/s10529-005-4680-7

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  • DOI: https://doi.org/10.1007/s10529-005-4680-7

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