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MiR-30c-5p-Targeted Regulation of GNAI2 Improves Neural Function Injury and Inflammation in Cerebral Ischemia-Reperfusion Injury

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Abstract

MiRNAs are related to neuronal proliferation and apoptosis following cerebral ischemia-reperfusion injury (CIRI). This study focused on miR-30c-5p in the disease. An oxygen-glucose deprivation/re-oxygenation (OGD/R) model was prepared in HT22 cells and transfected to overexpress miR-30c-5p and G Protein Subunit Alpha I2 (GNAI2) respectively or co-transfected to silence miR-30c-5p and GNAI2. Meanwhile, a middle cerebral artery occlusion (MCAO) model was constructed in mice, and miR-30c-5p and GNAI2 were silenced in vivo simultaneously. The mice were evaluated for neurological damage, apoptosis, and inflammation. HT22 cells were tested for cytotoxicity, proliferation, apoptosis, and inflammatory factors. The interaction between miR-30c-5p and GNAI2 was predicted, analyzed, and confirmed. MiR-30c-5p was found to be downregulated in both experimental models. miR-30c-5p reduced lactate dehydrogenase production, inflammatory response, inhibit apoptosis, and enhanced neuronal proliferation, while GNAI2 overexpression showed the opposite results. Downregulated miR-30c-5p worsened neurological function, apoptosis, and inflammation of MCAO mice while silencing GNAI2 attenuated the influence of downregulated miR-30c-5p. MiR-30c-5p can improve neuronal apoptosis and inflammatory response caused by CIRI and is neuroprotective by targeting GNAI2, providing a new target for treating CIRI.

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Data Availability

The datasets used and/or analyzed during the present study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Neurology, Yichun People’s Hospital of Jiangxi Province, No. 1061 Jinxiu Avenue, Yuanzhou District, Yichun City, Jiangxi Province, 336000, China

    Xinbo Deng, Ying Zeng & Dan Ding

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  1. Xinbo Deng
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  2. Ying Zeng
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  3. Dan Ding
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Contributions

XD designed the study, performed animal experiments, and wrote the manuscript. YZ performed in vitro assays, analyzed, and interpretated data. DD revised the manuscript, provided technical support, and supervised the project. All authors have read and approved the final manuscript.

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Correspondence to Dan Ding.

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Ethics Approval

The present study was approved by the Animal experiments were approved by Yichun People’Hospital of Jiangxi Province Animal Experimental Ethics Committee. and all procedures complied with the National Institutes of Health Guide for the Use of Laboratory Animals.

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The authors declare no competing interests.

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Deng, X., Zeng, Y. & Ding, D. MiR-30c-5p-Targeted Regulation of GNAI2 Improves Neural Function Injury and Inflammation in Cerebral Ischemia-Reperfusion Injury. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04802-5

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