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Therapeutic Benefits of Tuna Oil by In Vitro and In Vivo Studies Using a Rat Model of Acetic Acid-Induced Ulcerative Colitis

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Abstract

Ulcerative colitis (UC), an inflammation of the colon lining, represents the main form of inflammatory bowel disease IBD. Nutritional therapy is extremely important in the management of ulcerative colitis. Fish oil contains long-chain omega-3 polyunsaturated fatty acids, which have beneficial effects on health, including anti-inflammatory effects. This study aims to investigate the benefits of bluefin tuna oil extracted by the Soxhlet method in vitro by determining the anti-radical and anti-inflammatory activities and in vivo by evaluating the preventive and curative effects. The experiments were carried out using two doses of oil (100 and 260 mg/kg) and glutamine (400 and 1000 mg/kg) on the acetic acid-induced UC model. UC has been induced in Wistar rats by intrarectal administration of a single dose of 1 mL acetic acid (5% v/v in distilled water). The obtained results indicate that tuna oil and glutamine have a significant anti-free radical effect. Tuna oil has a marked anti-inflammatory power based on membrane stabilization and inhibiting protein denaturation. The reduction of various UC parameters, such as weight loss, disease activity score DAS, and colonic ulceration in rats pre-treated with tuna oil and glutamine, demonstrate that these treatments have a significant effect on UC. Total glutathione GSH, superoxide dismutase SOD, and catalase activities are significantly restored in the tuna oil and glutamine groups, while lipid peroxidation has been markedly reduced.

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Abbreviations

UC:

ulcerative colitis

IBD:

inflammatory bowel disease

WHO:

World Health Organization

ROS:

reactive oxygen species

PRDX2:

peroxiredoxin 2

GSH:

glutathione

TNF-α:

tumor necrosis factor

SOD:

superoxide dismutase

GPX:

glutathione peroxidase

CAT:

catalase

LOOH:

lipid hydroperoxide

HRQoL:

health-related quality of life

EPA:

eicosapentaenoic acid

DHA:

docosahexaenoic acid

IL-6:

interleukin 6

TO:

tuna oil

GC-MS:

gas chromatography coupled to mass spectrophotometer

AMDIS:

automated mass spectral deconvolution and identification system

HPLC:

high-performance liquid chromatography

FLD:

fluorescence detector

DPPH:

2,2-diphenyl-1-picrylhydrazyl

DMSO:

dimethyl sulfoxide

GLu:

glutamine

EDTA:

ethylenediaminetetraacetic acid

RSA%:

radical scavenging capacity

IC50 :

Half maximal inhibitory concentration

DS:

Diclofenac sodium

PMP:

percentage of membrane stabilization or protection

NC:

negative control group

PC:

positive control

SUL:

sulfasalazine

AA:

acetic acid

DAI:

disease activity index

MDA:

oxidative stress malondialdehyde

ELISA:

enzyme-linked immunosorbent assay

PMS:

post-mitochondrial supernatant

TCA:

trichloroacetic acid

H&E:

hematoxylin-eosin

BTO:

bluefin tuna oil

ARA:

arachidonic acid

COX:

cyclooxygenase

LOX:

lipoxygenase

NSAIDs:

non-steroidal anti-inflammatory drugs

PGs:

prostaglandins

TXBs:

thromboxanes

HETEs:

hydroxyeicosatetraenoic acids

LXs:

lipoxins

LTs:

leukotrienes

RvEs:

E-series resolvins

RvDs:

D-series resolvins

MaRs:

maresins

PDs:

protectins

NF-κB:

nuclear factor-κB chemokines

ω-3 PUFAs:

omega-3 polyunsaturated fatty acids

ω-6 PUFAs:

omega-6 polyunsaturated fatty acids

TNBS:

2,4,6-trinitrobenzenesulfonic acid

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Acknowledgements

The author would like to thank Prince Sultan University for their support.

Funding

This work is supported by the Ministry of Higher Education and Scientific Research, Abdelhamid Ibn Badis University, Mostaganem, Algeria. As a part of the Research Project Projets de Recherche-Formation Universitaire (PRFU). Code: D00L01UN270120200002 “The contribution of the intestinal microbiota to the physiological balance of the body.”

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Authors and Affiliations

  1. Laboratory of Bioeconomics, Food safety and Health, Faculty of Natural Sciences and Life, Abdelhamid Ibn Badis University of Mostaganem, 188, 27000, Mostaganem, BP, Algeria

    Abla Bouhend & Soumia Keddari

  2. Laboratory of Beneficial Microorganisms, Functional Food and Health (LMBAFS), Faculty of Natural and Life Sciences, Abdelhamid Ibn Badis University, Mostaganem, Algeria

    Imen Yahla

  3. Laboratory of Anapathology Histology, University Hospital Centre, Mostaganem, Algeria

    Omar Sadouki

  4. Department of Mathematics and Sciences, College of Humanities and Sciences, Prince Sultan University, Riyadh, Saudi Arabia

    Mohamed Bououdina

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  1. Abla Bouhend
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by AB and SK. The first draft of the manuscript was written by AB and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Conceptualization: SK and AB; methodology: SK and AB; formal analysis and investigation: SK, AB, and YI; writing—original draft preparation: AB, SK, and MB; writing—review and editing: AB, SK, and MB; funding acquisition: AB and SK; resources: AB, SK, and OS; supervision: SK

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Correspondence to Soumia Keddari or Mohamed Bououdina.

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Ethical Approval

All experimental procedures and animal protocols were approved by the Animal Protection Ethics Committee of the Faculty of Natural and Life Sciences, Abdelhamid Ibn Badis University, Algeria.

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Bouhend, A., Keddari, S., Yahla, I. et al. Therapeutic Benefits of Tuna Oil by In Vitro and In Vivo Studies Using a Rat Model of Acetic Acid-Induced Ulcerative Colitis. Appl Biochem Biotechnol (2023). https://doi.org/10.1007/s12010-023-04736-y

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