Abstract
Brown cotton is a kind of naturally colored cotton. Because of less processing and little dying, it is more friendlier to environment than white cotton. For brown cotton, pigment accumulation in fiber is one of the most important characteristics. In this study, we selected a brown fiber line and a white fiber cultivar to determine the factor that affects the pigmentation in brown fiber. Accordingly, fibers were collected to verify the presence of PAs by p-dimethylaminocinnamaldehyde (DMACA) and toluidine blue O (TBO) staining. The PAs content and related genes expressions were determined. As a result, there were obvious differences on the aspect of PAs synthesis in fiber between white cotton and brown cotton. For white fiber, the PAs content reached maximum at 5 DPA, and then gradually decreased to zero. But for brown fiber, the PAs content was increased from 5 to 15 DPA stage, and reached the maximum at the 15 DPA stage, then gradually decreased from 15 to 40 DPA stage. On the contrary, in white cotton, PAs were synthesized in the whole developmental stage from 5 to 40 DPA. And PAs content in brown fiber were far more than that in white fiber, which may be the reason why the brown pigment accumulated in brown fiber.
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Abbreviations
- PAs:
-
Proanthocyanidins
- DPA:
-
Days post anthesis
- DMACA:
-
p-Dimethylaminocinnamaldehyde
- TBO:
-
Toluidine blue O
- CHS:
-
Chalcone synthase
- DFR:
-
Dihydroflavonol reductase
- ANS:
-
Anthocyanidin synthase
- F3H:
-
Flavanone 3-hydroxylase
- ANR:
-
Anthocyanidin reductase
- PUB:
-
Polyubiquitin
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Acknowledgments
The present investigation is financially supported by the grant from the Doctoral Programs Foundation of the Ministry of Education of People’s Republic of China (No. 200803640002), Eleventh Five-Year Plan of Anhui Province (No. 07010302133) and Educational Commission of Anhui Province (No. KJ2011A103) .
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Communicated by K. Trebacz.
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Li, T., Fan, H., Li, Z. et al. The accumulation of pigment in fiber related to proanthocyanidins synthesis for brown cotton. Acta Physiol Plant 34, 813–818 (2012). https://doi.org/10.1007/s11738-011-0858-x
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DOI: https://doi.org/10.1007/s11738-011-0858-x