Abstract
This study aimed to develop an aptamer labeled nanozyme-based ELISA method based on ampicillin/aptamer and peroxidase activity of gold nanoparticles (AuNPs) to detect ampicillin residue in milk. The commercially available ampicillin conjugated bovine serum albumin (ampicillin/BSA) was used as a ‘coating antigen.’ The aptamers labeled with AuNPs (Au-apt) were prepared and used as an ‘enzyme-labeled antibody’ to detect ampicillin in milk. A positive correlation was observed between the amounts of ampicillin residue and competitively bounded Au-apt. The remaining Au-apt bounded ampicillin/BSA was negatively associated with UV absorption values. The materials are easy to be prepared in the present method. This study simplified the material preparation and demonstrated high a selectivity, a low detection limit, and a good batch stability. The method developed in this study has the potential to improve the monitoring method of ampicillin residue in milk.
Graphical abstract
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Adegoke O, Zolotovskaya S, Abdolvand A, Daeid NN (2020) Biomimetic graphene oxide-cationic multi-shaped gold nanoparticle-hemin hybrid nanozyme: Tuning enhanced catalytic activity for the rapid colorimetric apta-biosensing of amphetamine-type stimulants. Talanta 216:120990. https://doi.org/10.1016/j.talanta.2020.120990
Aghaei A, Jazi ME, Mlsna TE, Kamyabi MA (2019) A novel method for the preconcentration and determination of ampicillin using electromembrane microextraction followed by high-performance liquid chromatography. J Sep Sci 42(18):3002–3008. https://doi.org/10.1002/jssc.201900016
Ang CYW, Luo WH (1997) Rapid determination of ampicillin in bovine milk by liquid chromatography with fluorescence detection. J Aoac Int 80(1):25–30. https://doi.org/10.1093/jaoac/80.1.25
Aqda TG, Behkami S, Raoofi M, Bagheri H (2019) Graphene oxide-starch-based micro-solid phase extraction of antibiotic residues from milk samples. J Chromatogr A 1591:7–14. https://doi.org/10.1016/j.chroma.2018.11.069
Bagheri AR, Ghaedi M (2020) Magnetic metal organic framework for pre-concentration of ampicillin from cow milk samples. J Pharm Anal 10(4):365–375. https://doi.org/10.1016/j.jpha.2020.02.006
Borghei Y-S, Hosseinkhani S (2019) Aptamer-based colorimetric determination of early-stage apoptotic cells via the release of cytochrome c from mitochondria and by exploiting silver/platinum alloy nanoclusters as a peroxidase mimic. Microchim Acta 186(12):845. https://doi.org/10.1007/s00604-019-3977-5
Boriachek K, Masud MK, Palma C, Phan H-P, Yamauchi Y, Hossain MSA, Nguyen N-T, Salomon C, Shiddiky MJA (2019) Avoiding pre-isolation step in exosome analysis: Direct isolation and sensitive detection of exosomes using gold-loaded nanoporous ferric oxide nanozymes. Anal Chem 91(6):3827–3834. https://doi.org/10.1021/acs.analchem.8b03619
Camara M, Gallego-Pico A, Garcinuno RM, Fernandez-Hernando P, Durand-Alegria JS, Sanchez PJ (2013) An HPLC-DAD method for the simultaneous determination of nine beta-lactam antibiotics in ewe milk. Food Chem 141(2):829–834. https://doi.org/10.1016/j.foodchem.2013.02.131
Chiappetta G, Gandino L (1998) Rapid methods for beta-lactam antibiotics determination in milk: A comparison study between four commercial kits. Industrie Alimentari 37(369):455–457
Cliquet P, Goddeeris BM, Okerman L, Cox E (2007) Production of penicillin-specific polyclonal antibodies for a group-specific screening ELISA. Food Agri Immunol 18(3–4):237–252. https://doi.org/10.1080/09540100701802908
Haiss W, Thanh NTK, Aveyard J, Fernig DG (2007) Determination of size and concentration of gold nanoparticles from UV-Vis spectra. Anal Chem 79(11):4215–4221. https://doi.org/10.1021/ac0702084
Li N, Feng F, Yang B, Jiang P, Chu X (2014) Simultaneous determination of beta-lactam antibiotics and beta-lactamase inhibitors in bovine milk by ultra performance liquid chromatography-tandem mass spectrometry. J Chromatogr B 945:110–114. https://doi.org/10.1016/j.jchromb.2013.11.044
Li F, Li X, Zhu N, Li R, Kang H, Zhang Q (2020) An aptasensor for the detection of ampicillin in milk using a personal glucose meter. Anal Method 12(26):3376–3381. https://doi.org/10.1039/D0AY00256A
Majumder S, Johari S (2018) Development of a gold-nano particle based novel dot immunobinding assay for rapid and sensitive detection of Banana bunchy top virus. J Virol Methods 255:23–28. https://doi.org/10.1016/j.jviromet.2018.01.015
Martins-Júnior H, Kussumi TA, Wang AY, Lebre DT (2007) A rapid method to determine antibiotic residues in milk using liquid chromatography coupled to electrospray tandem mass spectrometry. J Brazil Chem Soc 18(2):397–405. https://doi.org/10.1590/S0103-50532007000200023
Mercadal PA, Fraire JC, Motrich RD, Coronado EA (2018) Enzyme-free immunoassay using silver nanoparticles for detection of gliadin at ultralow concentrations. ACS Omega 3(2):2340–2350. https://doi.org/10.1021/acsomega.7b01840
Moura F, de Almeida FG, Lopes BR, Cass QB (2012) Quantification of ampicillin in bovine milk by coupled-column ultrahigh-performance liquid chromatography-tandem mass spectrometry. J Sep Sci 35(19):2615–2620. https://doi.org/10.1002/jssc.201200205
Panferov VG, Safenkova IV, Zherdev AV, Dzantiev BB (2020) Urchin peroxidase-mimicking Au@Pt nanoparticles as a label in lateral flow immunoassay: impact of nanoparticle composition on detection limit of clavibacter michiganensis. Microchim Acta 187(5):268. https://doi.org/10.1007/s00604-020-04253-3
Pavlov V, Xiao Y, Shlyahovsky B, Willner I (2004) Aptamer-functionalized Au nanoparticles for the amplified optical detection of thrombin. J Am Chem Soc 126(38):11768–11769. https://doi.org/10.1021/ja046970u
Peng J, Cheng G, Huang L, Wang Y, Hao H, Peng D, Liu Z, Yuan Z (2013) Development of a direct ELISA based on carboxy-terminal of penicillin-binding protein BlaR for the detection of beta-lactam antibiotics in foods. Anal Bioanal Chem 405(27):8925–8933. https://doi.org/10.1007/s00216-013-7311-5
Sahebi H, Konoz E, Ezabadi A, Niazi A, Ahmadi SH (2020) Simultaneous determination of five penicillins in milk using a new ionic liquid-modified magnetic nanoparticle based dispersive micro-solid phase extraction followed by ultra-performance liquid chromatography-tandem mass spectrometry. Microchem J 154:104605. https://doi.org/10.1016/j.microc.2020.104605
Samsonova ZV, Shchelokova OS, Ivanova NL, Rubtsova MY, Egorov AA (2005) Enzyme-linked immunosorbent assay of ampicillin in milk. Appl Biochem Microbiol 41(6):589–595. https://doi.org/10.1007/s10438-005-0107-4
Sheng Y-M, Liang J, Xie J (2020) Indirect competitive determination of tetracycline residue in honey using an ultrasensitive gold-nanoparticle-linked aptamer assay. Molecules 25(9):2144. https://doi.org/10.3390/molecules25092144
Soledad-Rodriguez B, Fernandez-Hernando P, Garcinuno-Martinez RM, Durand-Alegria JS (2017) Effective determination of ampicillin in cow milk using a molecularly imprinted polymer as sorbent for sample preconcentration. Food Chem 224:432–438. https://doi.org/10.1016/j.foodchem.2016.11.097
Song K-M, Jeong E, Jeon W, Cho M, Ban C (2012) Aptasensor for ampicillin using gold nanoparticle based dual fluorescence-colorimetric methods. Anal Bioanal Chem 402(6):2153–2161. https://doi.org/10.1007/s00216-011-5662-3
Taghdisi SM, Danesh NM, Nameghi MA, Rarnezani M, Alibolandi M, Abnous K (2019) An electrochemical sensing platform based on ladder-shaped DNA structure and label-free aptamer for ultrasensitive detection of ampicillin. Biosens Bioelectron 133:230–235. https://doi.org/10.1016/j.bios.2019.03.044
Tang MQ, Xie J, Rao LM, Kan Y-J, Luo P, Qing L-S (2021) Advances in aptamer-based sensing assays for C-reactive protein. Anal Bioanal Chem. https://doi.org/10.1007/s00216-021-03674-0
Toh SY, Citartan M, Gopinath SCB, Tang T-H (2015) Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay. Biosens Bioelectron 64:392–403. https://doi.org/10.1016/j.bios.2014.09.026
Wang Q-L, Li J, Li X-D, Ding L-S, Xie J, Qing L-S (2017a) A simple nano-SiO2-based ELISA method for residue detection of 2,4-dichlorophenoxyacetic acid in bean sprouts. Food AnalMethod 10(5):1500–1506. https://doi.org/10.1007/s12161-016-0709-x
Wang Q-L, Li J, Li X-D, Tao W-J, Ding L-S, Luo P, Qing L-S (2017b) An efficient direct competitive nano-ELISA for residual BSA determination in vaccines. Anal Bioanal Chem 409(19):4607–4614. https://doi.org/10.1007/s00216-017-0403-x
Wang Q-L, Xie J, Li X-D, Ding L-S, Liang J, Luo P, Qing L-S (2017c) Development of a nano-SiO2 based enzyme-linked ligand binding assay for the determination of ibuprofen in human urine. Talanta 167:617–622. https://doi.org/10.1016/j.talanta.2017.03.011
Wang J, Ma K, Yin H, Zhou Y, Ai S (2018) Aptamer based voltammetric determination of ampicillin using a single-stranded DNA binding protein and DNA functionalized gold nanoparticles. Microchim Acta 185(1):68. https://doi.org/10.1007/s00604-017-2566-8
Wang T, Yin H, Zhang Y, Wang L, Du Y, Zhuge Y, Ai S (2019) Electrochemical aptasensor for ampicillin detection based on the protective effect of aptamer-antibiotic conjugate towards DpnII and Exo III digestion. Talanta 197:42–48. https://doi.org/10.1016/j.talanta.2019.01.010
Wang Q-L, Huang W-X, Zhang P-J, Chen L, Lio C-K, Zhou H, Qing L-S, Luo P (2020a) Colorimetric determination of the early biomarker hypoxia-inducible factor-1 alpha (HIF-1α) in circulating exosomes by using a gold seed-coated with aptamer-functionalized Au@Au core-shell peroxidase mimic. Microchim Acta 187(1):61. https://doi.org/10.1007/s00604-019-4035-z
Wang TT, kit Lio C, Huang H, Wang RY, Zhou H, Luo P, Qing LS (2020) A feasible image-based colorimetric assay using a smartphone RGB camera for point-of-care monitoring of diabetes. Talanta 206:120211. https://doi.org/10.1016/j.talanta.2019.120211
Wu N, Luo Z, Ge Y, Guo P, Du K, Tang W, Du W, Zeng A, Chang C, Fu Q (2016) A novel surface molecularly imprinted polymer as the solid-phase extraction adsorbent for the selective determination of ampicillin sodium in milk and blood samples. J Pharm Anal 6(3):157–164. https://doi.org/10.1016/j.jpha.2016.01.004
Xie J, Tang M-Q, Chen J, Zhu Y-H, Lei C-B, He H-W, Xu X-H (2020) A sandwich ELISA-like detection of C-reactive protein in blood by citicoline-bovine serum albumin conjugate and aptamer-functionalized gold nanoparticles nanozyme. Talanta 217:121070. https://doi.org/10.1016/j.talanta.2020.121070
Yu ZG, Lai RY (2018) A reagentless and reusable electrochemical aptamer-based sensor for rapid detection of ampicillin in complex samples. Talanta 176:619–624. https://doi.org/10.1016/j.talanta.2017.08.057
Zhu J, Li F, Zhao L, Li X, Liu Y (2019) Magnetic beads-based electrochemical aptasensor for the detection of ampicillin in milk. Food Sci 40(24):294–299. https://doi.org/10.7506/spkx1002-6630-20180923-236
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
On behalf of all authors, the corresponding author states that there is no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Zhang, X., Sun, W. & Cheng, Y. Aptamer labeled nanozyme-based ELISA for ampicillin residue detection in milk. Chem. Pap. 76, 3077–3085 (2022). https://doi.org/10.1007/s11696-022-02084-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11696-022-02084-5