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Dispersive solid phase extraction coupled with chromogenic reaction and UV–Vis spectrophotometry for the investigation of storage stability of proanthocyanidins in blueberry

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Abstract

Proanthocyanidins (PCs), known as natural antioxidants capable of scavenging free radicals, are rich in blueberry as oligomeric proanthocyanidins (OPCs). Aim to efficiently determine the total PCs in blueberry, a simple method of dispersive solid phase extraction (DSPE) followed by UV–Vis spectrophotometry detection was developed, in which C18-bonded silica microspheres were used as absorbent to purify the targets, and vanillin–HCl was applied for chromogenic reaction. After systematic optimization of DSPE conditions, reliable determination of total PCs was achieved. Though the sensitivity of this method was not comparable to those based on high performance liquid chromatography or mass spectrometry, it was simple and reliable for the analysis of PCs-rich fruits. With this method, impacts of storage conditions including time and temperature, as well as the freshness on total PCs concentration in blueberry were investigated. The results revealed that with the increase of storage time or temperature, the total PCs decreased accordingly. Meanwhile, the highest concentration of total PAs was found in the freshest blueberries stored at a low temperature of 4 °C, which supported that the low temperature was favorable for blueberry storage, showing the practical significance for the storage of PCs-rich fruits.

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Acknowledgements

This work was supported by National Undergraduate Training Program for Innovation and Entrepreneurship from Dalian University of Technology.

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  1. School of Ocean Science and Technology, Dalian University of Technology, Panjin, 124221, China

    Lingchuan Hao, Wenxue Xu & Minghuo Wu

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  1. Lingchuan Hao
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Hao, L., Xu, W. & Wu, M. Dispersive solid phase extraction coupled with chromogenic reaction and UV–Vis spectrophotometry for the investigation of storage stability of proanthocyanidins in blueberry. Food Measure 18, 1734–1739 (2024). https://doi.org/10.1007/s11694-023-02303-x

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