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Microencapsulation and invitro characterization of Bifidobacterium animalis for improved survival

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Abstract

Ciceritol is a prebiotic that improves the gastrointestinal health and viability of probiotics. Ciceritol can be used to improve the structural defects of sodium alginate-based beads and the viability of Bifidobacterium animalis. The present study focused on ciceritol extraction to encapsulate B. animalis in alginate and alginate-ciceritol matrix to enhance the survival of probiotic after exposure to simulated gastric conditions. Ciceritol and sodium alginate were used to encapsulate B. animalis with different formulations i.e.., C0SA2 (0%:2%), C1SA2 (0.5%:2%), C2SA2 (1%:2%), C3SA2 (1.5%:2%) and C4SA2 (2%:2%). Free cells and encapsulated cells were investigated for their survival in gastrointestinal conditions. The addition of prebiotics (ciceritol) significantly (p < 0.05) improved the survival of probiotics. The incorporation of ciceritol in alginate provided better protection and survival (> 106 CFU/mL) as compared to free cells. All the encapsulation treatments showed good storage stability after 30 days and maximum viable count was observed in C4SA2. Results revealed that the efficiency of co-encapsulation to improve the survival of B. animalis in harsh gastrointestinal conditions.

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Acknowledgements

The authors are grateful to the National Institute of Food Science and Technology, University of Agriculture Faisalabad, Pakistan for providing a conducive environment for research.

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Authors and Affiliations

  1. College of Rehabilitation and Allied Health Sciences, Riphah International University Faisalabad, Faisalabad, Punjab, 38040, Pakistan

    Muhammad Azam & Muhammad Afzaal

  2. National Institute of Food Science and Technology, University of Agriculture Faisalabad, Faisalabad, 38040, Pakistan

    Muhammad Azam, Muhammad Saeed, Wahab Ali Khan, Hafiz Tayub Hussain & Muhammad Asif

  3. Center of Excellence for Olive Research and Training, Barani Agricultural Research Institute, Chakwal, 48800, Pakistan

    Iqra Yasmin

  4. Department of Food Sciences, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, 38000, Pakistan

    Sheraz Ahmed

  5. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Muhammad Waheed Iqbal

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  1. Muhammad Azam
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  2. Muhammad Saeed
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  3. Iqra Yasmin
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  4. Muhammad Afzaal
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  9. Muhammad Asif
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Correspondence to Muhammad Azam.

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Azam, M., Saeed, M., Yasmin, I. et al. Microencapsulation and invitro characterization of Bifidobacterium animalis for improved survival. Food Measure 15, 2591–2600 (2021). https://doi.org/10.1007/s11694-021-00839-4

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  • DOI: https://doi.org/10.1007/s11694-021-00839-4

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