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Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice

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Chinese Journal of Integrative Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the therapeutic effects of total glucosides of paeony (TGP) on psoriasis based on the immunomodulatory effect of dermal mesenchymal stem cells (DMSCs).

Methods

A total of 30 male BALB/c mice were divided into 6 groups (n=5 in each) by a random number table method, including control, psoriasis model (model, 5% imiquimod cream 42 mg/d), low-, medium- and high-dose TGP (50, 100, and 200 mg/kg, L, M-, and H-TGP, respectively), and positive control group (2.5 mg/kg acitretin). After 14 days of continuous administration, the skin’s histopathological changes, apoptosis, secretion of inflammatory cytokines, and proportion of regulatory T cells (Treg) and T helper cell 17 (Th17) were evaluated using hematoxylin-eosin (HE) staining, TdT-mediated dUTP nick end labeling staining, enzyme-linked immunosorbent assay, and flow cytometry, respectively. DMSCs were further isolated from the skin tissues of normal and psoriatic mice, and the cell morphology, phenotype, and cycle were observed. Furthermore, TGP was used to treat psoriatic DMSCs to analyze the effects on the DMSCs immune regulation.

Results

TGP alleviated skin pathological injury, reduced epidermis layer thickness, inhibited apoptosis, and regulated the secretion of inflammatory cytokines and the proportion of Treg and Th17 in the skin tissues of psoriatic mice (P<0.05 or P<0.01). There was no significant difference in cell morphology and phenotype between control and psoriatic DMSCs (P>0.05), however, more psoriatic DMSCs remained in G0/G1 phase compared with the normal DMSCs (P<0.01). TGP treatment of psoriatic DMSCs significantly increased cell viability, decreased apoptosis, relieved inflammatory response, and inhibited the expression of toll-like receptor 4 and P65 (P<0.05 or P<0.01).

Conclusion

TGP may exert a good therapeutic effect on psoriasis by regulating the immune imbalance of DMSCs.

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Availability of Data and Materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Dermatology, Hebei Province Hospital of Chinese Medicine, Shijiazhuang, 050011, China

    Ming-jun Lei, Fan Bai, Qing-yun Zhang, Qing-qing Yang & Zan Tian

Authors
  1. Ming-jun Lei
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  2. Fan Bai
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  3. Qing-yun Zhang
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  4. Qing-qing Yang
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  5. Zan Tian
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Contributions

Lei MJ, Bai F and Tian Z designed the research; Lei MJ and Bai F performed the research; Zhang QY and Yang QQ analyzed the data; Lei MJ, Bai F and Tian Z wrote the paper. All authors agreed to publish the final version.

Corresponding author

Correspondence to Zan Tian.

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Conflict of Interest

The authors declare that they have no conflict of interest.

Supported by Hebei Provincial Administration of Traditional Chinese Medicine (No. 2020016)

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Lei, Mj., Bai, F., Zhang, Qy. et al. Total Glucosides of Paeony Regulate Immune Imbalance Mediated by Dermal Mesenchymal Stem Cells in Psoriasis Mice. Chin. J. Integr. Med. 29, 517–525 (2023). https://doi.org/10.1007/s11655-023-3737-y

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  • DOI: https://doi.org/10.1007/s11655-023-3737-y

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