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Immunomodulatory effect of psoriasis-derived dermal mesenchymal stem cells on TH1/TH17 cells

  • Investigative Report
  • Published:
European Journal of Dermatology Aims and scope

Abstract

Background

T cell-mediated inflammation plays an important role in the development of psoriasis. Mesenchymal stem cells (MSCs) are a population of multipotent cells that regulate the T cell-mediated immune response.

Objectives

To investigate the effects of psoriatic dermal mesenchymal stem cells (p-DMSCs) on proliferation, apoptosis and differentiation of T cells.

Materials & Methods

p-DMSCs and normal DMSCs (n-DMSCs) were isolated from psoriatic skin and normal healthy controls, respectively, and co-cultured with activated T cells isolated from healthy volunteers using a Transwell system. Proliferation and apoptosis of T cells were assessed by cell count and flow cytometry, respectively. Expression levels of transcription factors associated with subtypes of T cells and cytokines were measured by qRT-PCR and western blot.

Results

Both p-DMSCs and n-DMSCs inhibited T cell proliferation and cytokine production. Similarly, the presence of p-DMSCs and n-DMSCs decreased the expression levels of both T-bet and ROR-γt in T cells. However, n-DMSCs exhibited a stronger inhibitory effect than p-DMSCs on T cell proliferation, cytokine production, and T-bet and ROR-γt expression.

Conclusion

These results suggest that the effect of p-DMSCs on T cell function could contribute, at least in part, to the pathogenesis of psoriasis.

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Acknowledgements and disclosures

Acknowledgements: this work was supported by the National Natural Science Foundation of China (Grant no. 81773336, 81472888, 81401360). Conflicts of interest: none.

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Author notes

  1. These authors contributed equally

Authors and Affiliations

  1. Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, No. 5 Dong San Dao Xiang, Jiefang Road, Taiyuan, 030009, China

    Xincheng Zhao, Juanjuan Jiao, Xiaofang Li, Ruixia Hou, Junqin Li, Xuping Niu, Ruifeng Liu, Xiaohong Yang, Juan Li, Jiannan Liang, Ling Zhou, Qiang Wang, Wenjuan Chang, Fangdi Wang, Guohua Yin, Xinhua Li & Kaiming Zhang

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  1. Xincheng Zhao
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  2. Juanjuan Jiao
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  9. Juan Li
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  15. Guohua Yin
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  16. Xinhua Li
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  17. Kaiming Zhang
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Corresponding author

Correspondence to Kaiming Zhang.

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Zhao, X., Jiao, J., Li, X. et al. Immunomodulatory effect of psoriasis-derived dermal mesenchymal stem cells on TH1/TH17 cells. Eur J Dermatol 31, 318–325 (2021). https://doi.org/10.1684/ejd.2021.4050

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  • DOI: https://doi.org/10.1684/ejd.2021.4050

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