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Comprehensive Analysis of Key mRNAs and lncRNAs in Osteosarcoma Response to Preoperative Chemotherapy with Prognostic Values

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Abstract

Objective

Osteosarcoma is one of the most common types of bone sarcoma with a poor prognosis. However, identifying the predictive factors that contribute to the response to neoadjuvant chemotherapy remains a significant challenge.

Methods

A public data series (GSE87437) was downloaded to identify differentially expressed genes (DEGs) and differentially expressed lncRNAs (DElncRNAs) between osteosarcoma patients that do and do not respond to preoperative chemotherapy. Subsequently, functional analysis of the transcriptome expression profile, regulatory networks of DEGs and DElncRNAs, competing endogenous RNAs (ceRNA) and protein-protein interaction networks were performed. Furthermore, the function, pathway, and survival analysis of hub genes was performed and drug and disease relationship prediction of DElncRNA was carried out.

Results

A total of 626 DEGs, 26 DElncRNAs, and 18 hub genes were identified. However, only one gene and two lncRNAs were found to be suitable as candidate gene and lncRNAs respectively.

Conclusion

The DEGs, hub genes, candidate gene, and candidate lncRNAs screened out in this context were considered as potential biomarkers for the response to neoadjuvant chemotherapy of osteosarcoma.

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Author notes

  1. The authors contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, China

    Mi Li, Hao Li & Cai-hong Yang

  2. Oncology Department, Wuhan No.1 Hospital, Wuhan, 430030, China

    Wei-ting Cheng

  3. Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, China

    Zhi Zhang, Xiao-li Lu & Si-si Deng

  4. Institute of Experimental Immunology, University Clinic of Rheinische Friedrich-Wilhelms-University, Bonn, D-53127, Germany

    Jian Li

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  1. Mi Li
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  3. Hao Li
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Corresponding authors

Correspondence to Si-si Deng, Jian Li or Cai-hong Yang.

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The authors declare that there is no conflict of interest with any financial organization or corporation or individual that can inappropriately influence this work.

Additional information

The study was supported by the grant from the Research Foundation of Tongji Hospital (No. 2019B17).

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Li, M., Cheng, Wt., Li, H. et al. Comprehensive Analysis of Key mRNAs and lncRNAs in Osteosarcoma Response to Preoperative Chemotherapy with Prognostic Values. CURR MED SCI 41, 916–929 (2021). https://doi.org/10.1007/s11596-021-2430-2

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  • DOI: https://doi.org/10.1007/s11596-021-2430-2

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