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A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles

利用肽核酸和纳米银免标记比色检测原癌基因c-Myc mRNA

  • Article
  • Chemistry
  • Published:
Science Bulletin

Abstract

A label-free colorimetric protocol based on peptide nucleic acid/silver nanoparticles (PNA/AgNPs) has been initially proposed for specific recognition of mRNA. Making use of the controlled silver nanoparticles aggregation/dispersion by PNA/PNA–RNA complex, proto-oncogene c-Myc mRNA detection can be achieved. Moreover, the PNA/AgNPs platform can undergo color change in response to target c-Myc mRNA with single-base-mismatch sensitivity, which could further help in visually identify single nucleotide differences in target genes.

摘要

摘要 PNA具有较强的银纳米聚集能力,与核酸靶标RNA形成的双链能使团聚的银纳米再次得到分散。基于该原理,本文利用纳米银和PNA构建了一种灵敏的免标记比色检测原癌基因c-myc mRNA的方法。该方法简单、快速、成本低、纳摩尔级的样品无需仪器就可以观察到颜色的变化,而且还可以区分与靶标单个碱基错配的情况,有望在生物临床领域得到进一步广泛应用。

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Acknowledgments

This work was partially supported by the National Natural Science Foundation of China (21305058, 21205056, 21075058 and 21503104) and Tai-Shan Scholar Research Fund of Shandong Province.

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Authors and Affiliations

  1. Department of Chemistry, Liaocheng University, Liaocheng, 252059, China

    Xia Li, Juan Song, Bao-Li Chen, Bing Wang, Rui Li, Hui-Min Jiang & Chen-Zhong Li

  2. Key Laboratory of Food Nutrition and Safety, Ministry of Education, Tianjin University of Science and Technology, Tianjin, 300457, China

    Ji-Feng Liu

  3. Nanobioengineering/Bioelectronics Laboratory, Department of Biomedical Engineering, Florida International University, Miami, FL, 33174, USA

    Chen-Zhong Li

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  1. Xia Li
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  2. Juan Song
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Correspondence to Ji-Feng Liu or Chen-Zhong Li.

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Li, X., Song, J., Chen, BL. et al. A label-free colorimetric assay for detection of c-Myc mRNA based on peptide nucleic acid and silver nanoparticles. Sci. Bull. 61, 276–281 (2016). https://doi.org/10.1007/s11434-016-1004-3

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  • DOI: https://doi.org/10.1007/s11434-016-1004-3

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