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Vasculature-on-a-chip with stretchable sensor for recapitulating hemodynamics and electrochemical monitoring of endothelium

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Abstract

Vascular endothelium can perceive fluid shear stress (FSS) and cyclic circumferential stretch (CCS) caused by the pulsatile blood flow, and translate the hemodynamics into biochemical signals to regulate vascular pathophysiology. However, existing methods provide little information about the real-time biochemical responses of endothelium when exposed to dynamic FSS and CCS. Herein, a vasculature-on-a-chip integrated with stretchable sensing is engineered for recapitulating the hemodynamic milieus and in-situ monitoring biochemical responses of endothelial monolayer. The integrated device is developed by sandwiching a robust stretchable electrode between an upper fluidic channel and a lower pneumatic channel. The fluidic and pneumatic channels enable the simultaneous recapitulation of both FSS and CCS, and the integrated sensor exhibits excellent cell-adhesive capacity and electrochemical sensing stability even after long-term hemodynamic exposure. These allow real-time monitoring of hemodynamic form- and duration-dependent endothelium responses, and further efficacy investigation about a recommended drug for COVID-19, demonstrating the great potential in vascular disease and drug screening.

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Acknowledgements

This work was supported by the National Key Research and Development Program of China (2022YFA1104802) and the National Natural Science Foundation of China (22122408, 21721005, 22090051).

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Authors and Affiliations

  1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, 430072, China

    Wen-Ting Fan, Yi Zhao, Feng Hong, Yan-Ling Liu & Wei-Hua Huang

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  1. Wen-Ting Fan
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  2. Yi Zhao
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  3. Feng Hong
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  4. Yan-Ling Liu
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  5. Wei-Hua Huang
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Correspondence to Yan-Ling Liu or Wei-Hua Huang.

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Conflict of interest The authors declare no conflict of interest.

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Supporting information The supporting information is available online at chem.scichina.com and link.springer.com/journal/11426. The supporting materials are published as submitted, without typesetting or editing. The responsibility for scientific accuracy and content remains entirely with the authors.

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11426_2023_1741_MOESM1_ESM.pdf

Vasculature-on-a-chip with stretchable sensor for recapitulating hemodynamics and electrochemical monitoring of endothelium

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Fan, WT., Zhao, Y., Hong, F. et al. Vasculature-on-a-chip with stretchable sensor for recapitulating hemodynamics and electrochemical monitoring of endothelium. Sci. China Chem. 66, 3314–3322 (2023). https://doi.org/10.1007/s11426-023-1741-6

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  • DOI: https://doi.org/10.1007/s11426-023-1741-6

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