Abstract
Polysaccharides from Dendrobium officinale polysaccharides (DOPs) are the main bioactive components of Dendrobium officinale, which have the functions of antioxidation and immune regulation. However, it is not clear whether DOPs have any effect on the prevention of reproductive disorders induced by oxidative stress. The purpose of this study was to explore the protective effect of DOPs on reproductive oxidative stress injury in male mice and its possible mechanism. In this study, the mouse model of reproductive injury was established by intraperitoneal injection of cyclophosphamide (CTX). The reproductive function was evaluated by relative testicular mass, sperm parameters, and sex hormone levels. The oxidative stress level of male mice with reproductive injury treated with DOPs was analyzed by the levels of 8-hydroxydeoxyguanosine (8-OHdG), malondialdehyde (MDA), and nitric oxide (NO) in sperm. The expression of follicle-stimulating hormone receptor (FSHR) mRNA, androgen-binding (ABP) mRNA, and c-kit mRNA was detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to explore its mechanism. After CTX administration, the sperm density, sperm motility, normal sperm morphology, and sex hormone levels in mice were significantly lower than those in the control group (P < 0.05). At the same time, the expression of p53 protein was upregulated, and the expression of Bcl-2 protein was downregulated (P < 0.05). In addition, the expression of FSHR and ABP mRNA on Sertoli cells was also significantly inhibited (P < 0.05). DOPs can effectively reduce the oxidative stress injury of testicular tissue. After DOP treatment, the sperm quality and sex-related hormone levels of mice were significantly improved and positively correlated with the dose of DOPs (P < 0.05). Administration of DOPs can reduce the damage caused by oxidative stress by reducing the level of oxidative stress, improving the hormone environment in testes, and regulating the expression of specific genes in Sertoli cells and spermatogenic cells.
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Data availability
The dataset used in the current study is available from the corresponding author upon reasonable request.
Abbreviations
- ROS:
-
Reactive oxygen species
- DOPs:
-
Dendrobium officinale polysaccharides
- GPX:
-
Glutathione peroxidase
- MDA:
-
Malondialdehyde
- CTX:
-
Cyclophosphamide
- ELISA:
-
Enzyme-linked immunosorbent assay
- FSH:
-
Follicle-stimulating hormone
- LH:
-
Luteinizing hormone
- T:
-
Testosterone
- NO:
-
Nitric oxide
- OD:
-
Optical density
- GAPDH:
-
Glyceraldehyde 3-phosphate dehydrogenase
- LPO:
-
Lipid peroxidation
- INOS:
-
Inducible nitric oxide synthase
- HPG:
-
Hypothalamic-pituitary-gonadal
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Acknowledgements
Thanks Professor Chen Yujiang of the Department of Pathology of the First Affiliated Hospital of Guizhou University of Traditional Chinese Medicine and his team for their help in the pathological analysis of this study.
Funding
This study was supported by the Science and Technology Fund Project of the Guizhou Health Commission (gzwkj2021-211) and the Excellent Young Talents Plan of Guizhou Medical University ((2022)107).
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YM, BWC, WJZ, and SHX performed experiments and collected the data; WL, JH, and ML helped in the statistical evaluation of the data; PC, SWZ, and GYL participated in the discussion. YM and BWC helped in manuscript writing; KFT designed the study and wrote the manuscript. The corresponding author had full access to all the data in the study and had final responsibility for the decision to submit it for publication. All the authors contributed to the design of the study and the final manuscript.
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All mice were provided by the Experimental Animal Center of Guizhou Medical University and carried out under the guidance and approval of the Animal Ethics Committee of Guizhou Medical University (no. 202101430).
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Mu, ., Che, B., Tang, K. et al. Dendrobium officinale polysaccharides improved reproductive oxidative stress injury in male mice treated with cyclophosphamide. Environ Sci Pollut Res 30, 106431–106441 (2023). https://doi.org/10.1007/s11356-023-29874-y
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DOI: https://doi.org/10.1007/s11356-023-29874-y