Skip to main content
SpringerLink
Log in

Polymeric curcumin nanospheres for lysozyme aggregation inhibition, antibacterial, and wound healing applications

  • Smart Nanomaterials for Healthcare and Environmental Applications: Perspectives in Nanotoxicology
  • Published:
Environmental Science and Pollution Research Aims and scope Submit manuscript

Abstract

The present study reports highly stable polymeric nanoparticles comprising curcumin and polyvinylpyrrolidone, and then conjugated with gold nanoparticles, resulting in C-PVP and C-PVP-Au, respectively. The synthesized conjugates C-PVP and C-PVP-Au were investigated for amyloid aggregation inhibition activity, antimicrobial activity, and wound healing applications. The anti-amyloidogenic capacity of nanoconjugates were studied for model protein, hen egg-white lysozyme (HEWL). The ThT binding assay, fibril size measurement, and electron microscopy results revealed that conjugates suppress fibrillogenesis in HEWL. The highest amyloid inhibition activity obtained against C-PVP and C-PVP-Au was 31 μg.mL−1 and 30 μg.mL−1, respectively. The dissociation activity for amyloid aggregation was observed against Q-PVP and Q-PVP-Au at 29 μg.mL−1 and 27 μg.mL−1, respectively. The antibacterial studies show significant efficacy against Escherichia coli (E. coli) in the presence of C-PVP and C-PVP-Au. The substantial antibacterial potential of C-PVP@PVA and C-PVP-Au@PVA membranes shows promising wound healing applications. The PVA membranes with nanoparticles promote the antibacterial activity and wound healing activity in the Drosophila model. C-PVP-Au@PVA membrane healed the wound faster than the C-PVP@PVA, and it can be used for better results in wound healing. Thus, C-PVP-Au and C-PVP have higher bioavailability and stability and can act as multifunctional therapeutic agents for amyloid-related diseases and as wound healing agents.

C-PVP, and C-PVP-Au conjugates for inhibition of HEWL aggregation, antibacterial and wound healing activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13

Similar content being viewed by others

Data availability

 Not applicable.

References

  • Ahmad-Mansour N, Loubet P, Pouget C, Dunyach-Remy C, Sotto A, Lavigne J-P, Molle V (2021) Staphylococcus aureus toxins: an update on their pathogenic properties and potential treatments. Toxins 13:677

    Article  CAS  Google Scholar 

  • Alam P, Chaturvedi SK, Siddiqi MK, Rajpoot RK, Ajmal MR, Zaman M, Khan RH (2016) Vitamin k3 inhibits protein aggregation: implication in the treatment of amyloid diseases. Sci Rep 6:26759

    Article  CAS  Google Scholar 

  • Aleya L, Uddin MS (2020) Environmental pollutants and the risk of neurological disorders. Environ Sci Pollut Res 27:44657–44658

  • Alhosseini SN, Moztarzadeh F, Mozafari M, Asgari S, Dodel M, Samadikuchaksaraei A, Kargozar S, Jalali N (2012) Synthesis and characterization of electrospun polyvinyl alcohol nanofibrous scaffolds modified by blending with chitosan for neural tissue engineering. Int J Nanomed 7:25–34

    CAS  Google Scholar 

  • Antosova A, Bednarikova Z, Koneracka M, Antal I, Marek J, Kubovcikova M, Zavisova V, Jurikova A, Gazova Z (2019) Amino acid functionalized superparamagnetic nanoparticles inhibit lysozyme amyloid fibrillization. Chem A Eur J 25:7501–7514

    Article  CAS  Google Scholar 

  • Bag J, Mukherjee S, Tripathy M, Mohanty R, Shendha PK, Hota G, Mishra M (2023) Platinum as a novel nanoparticle for wound healing model in Drosophila melanogaster. J Clust Sci 34:1087–1098

  • Basniwal RK, Buttar HS, Jain V, Jain N (2011) Curcumin nanoparticles: preparation, characterization, and antimicrobial study. J Agric Food Chem 59:2056–2061

    Article  Google Scholar 

  • Betts JW, Sharili AS, La Ragione RM, Wareham DW (2016) In vitro antibacterial activity of curcumin–polymyxin B combinations against multidrug-resistant bacteria associated with traumatic wound infections. J Nat Prod 79:1702–1706

    Article  CAS  Google Scholar 

  • Brahmkhatri VP, Sharma N, Sunanda P, D’Souza A, Raghothama S, Atreya HS (2018) Curcumin nanoconjugate inhibits aggregation of N-terminal region (Aβ-16) of an amyloid beta peptide. New J Chem 42:19881–19892

    Article  CAS  Google Scholar 

  • Calabrese G, Molzahn C, Mayor T (2022) Protein interaction networks in neurodegenerative diseases: From physiological function to aggregation. J Biol Chem 298:102062

    Article  CAS  Google Scholar 

  • Cui L, Wang S, Zhang J, Wang M, Gao Y, Bai L, Zhang H, Ma G, Ba X (2019) Effect of curcumin derivatives on hen egg white lysozyme amyloid fibrillation and their interaction study by spectroscopic methods. Spectrochim Acta A Mol Biomol Spectrosc 223:117365

    Article  CAS  Google Scholar 

  • Esmaeili A, Beni AA (2014) A novel fixed-bed reactor design incorporating an electrospun PVA/chitosan nanofiber membrane. J Hazard Mater 280:788–796

    Article  CAS  Google Scholar 

  • Gangwar RK, Dhumale VA, Kumari D, Nakate UT, Gosavi S, Sharma RB, Kale S, Datar S (2012) Conjugation of curcumin with PVP capped gold nanoparticles for improving bioavailability. Mater Sci Eng C 32:2659–2663

    Article  CAS  Google Scholar 

  • Her S, Jaffray DA, Allen C (2017) Gold nanoparticles for applications in cancer radiotherapy: mechanisms and recent advancements. Adv Drug Deliv Rev 109:84–101

    Article  CAS  Google Scholar 

  • Howden BP, Giulieri SG, Wong Fok Lung T, Baines SL, Sharkey LK, Lee JYH, Hachani A, Monk IR, Stinear TP (2023) Staphylococcus aureus host interactions and adaptation. Nat Rev Microbiol

  • Jebahi S, Saoudi M, Farhat L, Oudadesse H, Rebai T, Kabir A, El Feki A, Keskes H (2015) Effect of novel curcumin-encapsulated chitosan–bioglass drug on bone and skin repair after gamma radiation: experimental study on a Wistar rat model. Cell Biochem Funct 33:150–159

    Article  CAS  Google Scholar 

  • Kakkar V, Saini K, Saini M, Kumar M, Narula P, Duggal I (2020) Comparison of therapeutic efficacy of nanoformulations of curcumin vs tetrahydrocurcumin in various disorders. In: Talegaonkar S, Rai M (eds) Nanoformulations in human health: challenges and approaches. Springer International Publishing, Cham, pp 377–401

    Chapter  Google Scholar 

  • Kaur S, Modi NH, Panda D, Roy N (2010) Probing the binding site of curcumin in Escherichia coli and Bacillus subtilis FtsZ–a structural insight to unveil antibacterial activity of curcumin. Eur J Med Chem 45:4209–4214

    Article  CAS  Google Scholar 

  • Kemp JA, Shim MS, Heo CY, Kwon YJ (2016) “Combo” nanomedicine: co-delivery of multi-modal therapeutics for efficient, targeted, and safe cancer therapy. Adv Drug Deliv Rev 98:3–18

    Article  CAS  Google Scholar 

  • Kumar R, Bauri S, Sahu S, Chauhan S, Dholpuria S, Ruokolainen J, Kesari KK, Mishra M, Gupta PK (2023) In vivo toxicological analysis of MnFe2O4@ poly (t BGE-alt-PA) composite as a hybrid nanomaterial for possible biomedical use. ACS Appl Bio Mater 6:1122–1132

  • Kurakula M, Rao G (2020) Pharmaceutical assessment of polyvinylpyrrolidone (PVP): as excipient from conventional to controlled delivery systems with a spotlight on COVID-19 inhibition. J Drug Deliv Sci Technol 60:102046

    Article  CAS  Google Scholar 

  • Lan W, He L, Liu Y (2018) Preparation and properties of sodium carboxymethyl cellulose/sodium alginate/chitosan composite film. Coatings 8:291

    Article  Google Scholar 

  • Lin X, Huang X, Zeng C, Wang W, Ding C, Xu J, He Q, Guo B (2019) Poly(vinyl alcohol) hydrogels integrated with cuprous oxide–tannic acid submicroparticles for enhanced mechanical properties and synergetic antibiofouling. J Colloid Interface Sci 535:491–498

    Article  CAS  Google Scholar 

  • Lindblom A, Kiszakiewicz C, Kristiansson E, Yazdanshenas S, Kamenska N, Karami N, Åhrén C (2022) The impact of the ST131 clone on recurrent ESBL-producing E. coli urinary tract infection: a prospective comparative study. Sci Reports 12:10048

    CAS  Google Scholar 

  • Liu H, Yu L, Dong X, Sun Y (2017) Synergistic effects of negatively charged hydrophobic nanoparticles and (-)-epigallocatechin-3-gallate on inhibiting amyloid β-protein aggregation. J Colloid Interface Sci 491:305–312

    Article  CAS  Google Scholar 

  • Lumbreras-Aguayo A, Meléndez-Ortiz HI, Puente-Urbina B, Alvarado-Canché C, Ledezma A, Romero-García J, Betancourt-Galindo R (2019) Poly(methacrylic acid)-modified medical cotton gauzes with antimicrobial and drug delivery properties for their use as wound dressings. Carbohyd Polym 205:203–210

    Article  CAS  Google Scholar 

  • Maiti S, Krishnan D, Barman G, Ghosh SK, Laha JK (2014) Antimicrobial activities of silver nanoparticles synthesized from Lycopersicon esculentum extract. J Anal Sci Technol 5:40

    Article  Google Scholar 

  • Mandal S, Debnath K (2020) Trehalose-conjugated, catechin-loaded polylactide nanoparticles for improved neuroprotection against intracellular polyglutamine aggregates. 21:1578–1586

  • Mathers AJ, Peirano G, Pitout JD (2015) The role of epidemic resistance plasmids and international high-risk clones in the spread of multidrug-resistant Enterobacteriaceae. Clin Microbiol Rev 28:565–591

    Article  CAS  Google Scholar 

  • Mieszawska AJ, Mulder WJ, Fayad ZA, Cormode DP (2013) Multifunctional gold nanoparticles for diagnosis and therapy of disease. Mol Pharm 10:831–847

    Article  CAS  Google Scholar 

  • Mukherjee S, Rananaware P, Brahmkhatri V, Mishra M (2023) Polyvinylpyrrolidone-curcumin nanoconjugate as a biocompatible, non-toxic material for biological applications. J Clust Sci 34:395–414

  • Mun S-H, Joung D-K, Kim Y-S, Kang O-H, Kim S-B, Seo Y-S, Kim Y-C, Lee D-S, Shin D-W, Kweon K-T, Kwon D-Y (2013) Synergistic antibacterial effect of curcumin against methicillin-resistant Staphylococcus aureus. Phytomedicine 20:714–718

    Article  CAS  Google Scholar 

  • Muniyappan N, Nagarajan NS (2014) Green synthesis of gold nanoparticles using Curcuma pseudomontana essential oil, its biological activity and cytotoxicity against human ductal breast carcinoma cells T47D. J Environ Chem Eng 2:2037–2044

    Article  CAS  Google Scholar 

  • Murugesan B, Arumugam M, Pandiyan N, Veerasingam M, Sonamuthu J, Samayanan S, Mahalingam S (2019) Ornamental morphology of ionic liquid functionalized ternary doped N, P, F and N, B, F-reduced graphene oxide and their prevention activities of bacterial biofilm-associated with orthopedic implantation. Mater Sci Eng C 98:1122–1132

    Article  CAS  Google Scholar 

  • Nejati K, Dadashpour M, Gharibi T, Mellatyar H, Akbarzadeh A (2022) Biomedical applications of functionalized gold nanoparticles: a review. J Clust Sci 33:1–16

    Article  CAS  Google Scholar 

  • Niedermayer S, Weiss V, Herrmann A, Schmidt A, Datz S, Müller K, Wagner E, Bein T, Bräuchle C (2015) Multifunctional polymer-capped mesoporous silica nanoparticles for pH-responsive targeted drug delivery. Nanoscale 7:7953–7964

    Article  CAS  Google Scholar 

  • Ow S-Y, Dunstan DE (2013) The effect of concentration, temperature and stirring on hen egg white lysozyme amyloid formation. Soft Matter 9:9692–9701

  • Parveen R, Shamsi TN, Fatima S (2017) Nanoparticles-protein interaction: role in protein aggregation and clinical implications. Int J Biol Macromol 94:386–395

    Article  CAS  Google Scholar 

  • Peresin MS, Vesterinen AH, Habibi Y, Johansson LS, Pawlak JJ, Nevzorov AA, Rojas OJ (2014) Crosslinked PVA nanofibers reinforced with cellulose nanocrystals: water interactions and thermomechanical properties. J Appl Polym Sci 131

  • Priyadarsini S, Mukherjee S, Mishra M (2020) Formulation of Drosophila food for various feeding studies. In: Fundamental approaches to screen abnormalities in Drosophila (pp 1–13), Springer US, New York

  • Rahman A, Saikia B, Gogoi CR, Baruah A (2022) Advances in the understanding of protein misfolding and aggregation through molecular dynamics simulation. Progress Biophys Mol Biol

  • Rajak BL, Kumar R, Gogoi M, Patra S (2020) Antimicrobial activity of nanomaterials. In: Daima HK, Pn N, Ranjan S, Dasgupta N, Lichtfouse E (eds) Nanoscience in medicine, vol 1. Springer International Publishing, Cham, pp 147–185

    Google Scholar 

  • Rananaware P, Pandit P, Naik S, Mishra M, Keri RS, Brahmkhatri VP (2022) Anti-amyloidogenic property of gold nanoparticle decorated quercetin polymer nanorods in pH and temperature induced aggregation of lysozyme. RSC Adv 12:23661–23674

    Article  CAS  Google Scholar 

  • Roopesh M, Jyothi MS, Velmurugan R, Hegde G, Soontarapa K, Keri RS (2022) Evaluation of wound healing effect of curcumin loaded OPL carbon nanospheres embedded chitosan membranes. J Polym Environ

  • Salehi B, Rodrigues CF (2021) Curcumin nanoformulations for antimicrobial and wound healing purposes

  • Sana SS, Dogiparthi LK, Gangadhar L, Chakravorty A, Abhishek N (2020) Effects of microplastics and nanoplastics on marine environment and human health. Environ Sci Pollut Res 27:44743–44756

  • Selkoe DJ (1999) Translating cell biology into therapeutic advances in Alzheimer’s disease. Nature 399:A23

    Article  CAS  Google Scholar 

  • Shiohara A, Prieto-Simon B, Voelcker NH (2021) Porous polymeric membranes: fabrication techniques and biomedical applications. J Mater Chem B 9:2129–2154

    Article  CAS  Google Scholar 

  • Shkodra-Pula B, Vollrath A, Schubert US, Schubert S (2020) Chapter 9 - Polymer-based nanoparticles for biomedical applications. In: Parak WJ, Feliu N (eds) Frontiers of nanoscience. Elsevier, pp 233–252

    Google Scholar 

  • Tang S, Wang W, Zhang X (2021) Direct visualization and profiling of protein misfolding and aggregation in live cells. Curr Opin Chem Biol 64:116–123

    Article  CAS  Google Scholar 

  • Terence MC, Faldini SB, de Miranda LF, Júnior AHM, de Castro PJ (2011) Preparation and characterization of a polymeric blend of PVP/PVAL for use in drug delivery system. J Biomed Nanotechnol 7:446–449

    Article  CAS  Google Scholar 

  • Trouiller AJ, Hebie S, El Bahhaj F, Napporn TW, Bertrand P (2015) Chemistry for oncotheranostic gold nanoparticles. Eur J Med Chem 99:92–112

    Article  CAS  Google Scholar 

  • Venkataprasanna K, Prakash J, Mathapati SS, Bharath G, Banat F, Venkatasubbu GD (2021) Development of chitosan/poly (vinyl alcohol)/graphene oxide loaded with vanadium doped titanium dioxide patch for visible light driven antibacterial activity and accelerated wound healing application. Int J Biol Macromol 193:1430–1448

    Article  CAS  Google Scholar 

  • Wang J-B, Wang Y-M, Zeng C-M (2011) Quercetin inhibits amyloid fibrillation of bovine insulin and destabilizes preformed fibrils. Biochem Biophys Res Commun 415:675–679

    Article  CAS  Google Scholar 

  • Yang Q, Wang K, Nie J, Du B, Tang G (2014) Poly (N-vinylpyrrolidinone) microgels: preparation, biocompatibility, and potential application as drug carriers. Biomacromol 15:2285–2293

    Article  CAS  Google Scholar 

Download references

Funding

This research was supported by the India and NANOMISSION project (SR/NM/NS-20/2014) and TARE-SERB. TAR/2018/000547.

Author information

Authors and Affiliations

  1. Nanomaterials for Drug Delivery and Therapeutics (NDT-Lab), Centre for Nano and Material Science, Jain University, Jain Global Campus, Bengaluru, 562112, Karnataka, India

    Pranita Rananaware, Rangappa Keri & Varsha Brahmkhatri

  2. Neural Developmental Biology Lab, Department of Life Science, NIT Rourkela, Odisha, Rourkela, 769008, India

    Samir Bauri & Monalisa Mishra

Authors
  1. Pranita Rananaware
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Samir Bauri
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rangappa Keri
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Monalisa Mishra
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Varsha Brahmkhatri
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

Conceptualization: Varsha Brahmkhatri; methodology: Pranita Rananaware; formal analysis and investigation: Pranita Rananaware; wound healing studies, biocompatibility, biofilm and minimum inhibition concentrations studies: Samir Bauri and Monalisa Mishra; writing—original draft preparation: Varsha Brahmkhatri and Pranita Rananaware; writing—review and editing: Pranita Rananaware, Rangappa S. Keri, and Varsha P. Brahmkhatri; supervision: Varsha Brahmkhatri.

Corresponding author

Correspondence to Varsha Brahmkhatri.

Ethics declarations

Ethics approval

Handling and experimental work with Drosophila fly model were carried out under protocols approved by the Department of Life Science, Neural Developmental Biology Lab, NIT Rourkela, Odisha, India, and following internationally established procedures.

Consent to participate

Not applicable.

Consent for publication

All authors consented to publish the results.

Competing interests

The authors declare no competing interests.

Additional information

Responsible Editor: Mohamed M. Abdel-Daim

Publisher's note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 2614 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rananaware, P., Bauri, S., Keri, R. et al. Polymeric curcumin nanospheres for lysozyme aggregation inhibition, antibacterial, and wound healing applications. Environ Sci Pollut Res (2023). https://doi.org/10.1007/s11356-023-29160-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11356-023-29160-x

Keywords

Search

Navigation