Abstract
Natural antioxidants have become vital to minimize macromolecular damage caused by Reactive Oxygen Species (ROS). This study investigated the antioxidant property of β-cryptoxanthin (β-CRX) extracted from Kocuria marina DAGII and its protective effect against macromolecular damages by generating ROS via two models: UV radiation and the Fenton reaction. β-cryptoxanthin exhibited the highest scavenging activity towards hydrogen peroxide radicals with an IC50 value of 38.30 ± 1.13 μg/ml, favoring the hydrogen atom transfer mechanism. The total antioxidant capacity value of 872.0101 ± 1.84 μg BHT/mg β-CRX indicated the cumulative ROS scavenging ability of β-cryptoxanthin. β-cryptoxanthin could protect against ROS-induced lipid peroxidation, protein oxidation, and DNA damage. The highest lipid peroxidation and protein oxidation inhibition values of β-cryptoxanthin against ROS were 99.371 ± 0.51% and 78.19 ± 0.15%, respectively. β-cryptoxanthin also showed a protective effect in maintaining DNA intactness against ROS-mediated DNA damage. Allium cepa test showed the non-genotoxic nature of β-cryptoxanthin and its protective effect against ROS genotoxic effects. A photo-stability study of β-cryptoxanthin toward UVA and UVB radiation showed a rapid bleaching result of UVB obeying pseudo-zero order kinetics with an average R2 value of 0.9897 and a higher k value (−6.3 × 10–11 ± 0.2 M/s) than UVA (k value −3.1 × 10–11 ± 0.17 M/s), signifying that UVB is more potent toward photo-degradation. The good SPF value of 23.1737 ± 0.15 showed the UV protection capability of β-cryptoxanthin. Thus, the present study suggests that β-cryptoxanthin could be a valuable antioxidant to protect against ROS-induced various macromolecular damages and act as a good UV protectant.
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All authors have equally contributed to this work. Daiji Brahma have performed material preparation, experiment, data collection, data analysis and original draft preparation, Debjani Dutta have conceptualized, supervised, investigated and formally analyzed the work. All the authors have read and approved the final manuscript.
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Brahma, D., Dutta, D. Evaluating β-cryptoxanthin antioxidant properties against ROS-induced macromolecular damages and determining its photo-stability and in-vitro SPF. World J Microbiol Biotechnol 39, 310 (2023). https://doi.org/10.1007/s11274-023-03747-5
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DOI: https://doi.org/10.1007/s11274-023-03747-5