Abstract
The present study aimed to evaluate the probiotic potential, α-amylase and α-glucosidase inhibitory effects, and β-galactosidase production of 19 non haemolytic lactic acid bacteria and bifidobacteria previously identified and isolated from honey bee gastrointestinal tract (BGIT) of Apis mellifera intermissa, honey, propolis and bee bread. The isolates were screened according to their high resistance to lysozyme and potent antibacterial activity. Our results indicated that among the 19 isolates, Limosilactobacillus fermentum BGITE12.2, Lactiplantibacillus plantarum BGITEC13, Limosilactobacillus fermentum BGITEC5.1 and Bifidobacterium asteroides BGITOB8, isolated from BGIT exhibited a good tolerance to 100 mg/mL lysozyme (> 82%), excellent tolerance to 0.5% bile salt [survival rate (SR) ≥ 83.19% ± 0.01], and a high SR (≥ 80.0%) under gastrointestinal tract conditions. The auto-aggregation ability was high (auto-aggregation index ranging from 67.14 ± 0.16 to 92.8% ± 0.03) for L. fermentum BGITE12.2, L. plantarum BGITEC13, and B. asteroides BGITOB8, and moderate for L. fermentum BGITEC5.1 (39.08% ± 0.11). Overall, the four isolates showed moderate co-aggregation capacity with pathogenic bacteria. They exhibited from moderate to high hydrophobicity towards toluene and xylene. The safety assessment revealed that the four isolates lacked gelatinase and mucinolytic activities. Also, they were susceptible to ampicillin, clindamycin, erythromycin, and chloramphenicol. Interestingly, the four isolates showed α-glucosidase and α-amylase inhibitory activities ranging from 37.08 ± 0.12 to 57.57% ± 0.1 and from 68.30 ± 0.09 to 79.42% ± 0.09, respectively. Moreover, L. fermentum BGITE12.2, L. plantarum BGITEC13, L. fermentum BGITEC5.1 isolates exhibited β-galactosidase activity over a wide range of 52.49 ± 0.24–746.54 ± 0.25 Miller Units. In conclusion, our findings suggest that the four isolates could be potential candidates for probiotics with interesting functional properties.
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Acknowledgements
We thank Pr. Karim Naghmouchi, from College of Clinical Pharmacy, Department of Pharmaceutical Chemistry, Al Baha University, Saudi Arabia., for suggesting us to investigate LAB & Bifido from honey bee. Dr. Lamia Thabet, from Traumatology and Great Burned Center, Tunis, Tunisia, for the gift of strains S. aureus, E. coli, K. pneumoniae, A. baumannii, and P. aeruginosa (collection of 2018).
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The authors are grateful to The Tunisian Ministry of Higher Education and Scientific Research for financial support for the Laboratory LR01ES05. The funder had no role in study design, data collection and interpretation, or the decision to submit the work for publication.
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HB-M designed the study, performed experiments and drafted the manuscript. M-OB-B and KB-M contributed to the manuscript revision. SR supervised the work, wrote and edited the manuscript. All authors read and approved the manuscript.
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Ben-Miled, H., Benoit-Biancamano, MO., Ben-Mahrez, K. et al. Alpha-amylase and alphaglucosidase inhibitory properties, beta-galactosidase activity, and probiotic potential of lactic acid bacteria and bifidobacteria from Apis mellifera intermissa and its products. World J Microbiol Biotechnol 39, 205 (2023). https://doi.org/10.1007/s11274-023-03648-7
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DOI: https://doi.org/10.1007/s11274-023-03648-7