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Effects of essential oil mixtures on expression of genes involved in lipogenesis and fatty acid oxidation in geese (Anser anser)

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Abstract

The use of essential oils has recently increased in the poultry sector. The aim of this study was to investigate the effects of essential oil mixture (juniper, mint, oregano and rosemary oil) on fatty acid oxidation and lipogenic gene expression in geese. Research groups were formed as C (control; no additives), EK1 (0.4 ml/l essential oil mixture supplemented) and EK2 (0.8 ml/l essential oil mixture supplemented). Relative expression levels of genes included in lipogenesis (ACCα, ChREBP, FASN, LXRα and SREBP-1) expression levels of genes included in fatty acid oxidation (ACOX1, CPT1, CPT1A, PPARα and PPARγ) were measured using RT-qPCR. Group EK1 upregulates the mRNA expression levels of genes involved in lipogenesis such as ACCα, ChREBP and SREBP-1, while it downregulates the mRNA expression in levels of all genes involved in fatty acid oxidation. Group EK2 increases the mRNA expression levels of genes involved in lipogenesis such as ACCα, FASN and SREBP-1, while it decreased mRNA expression at the levels of all genes involved in fatty acid oxidation, as in the other group. In the study, adding an essential oil mixture to drinking water is predicted to increase fatty liver because it upregulates genes related to fat synthesis (lipogenesis) and downregulates genes related to fat degradation (fatty acid oxidation).

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Authors and Affiliations

  1. Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Dicle University, 21200, Diyarbakır, Turkey

    Ozlem Durna Aydin & Oktay Kaplan

  2. Department of Genetics, Faculty of Veterinary Medicine, Kastamonu University, 37150, Kastamonu, Turkey

    Mustafa Hitit

  3. Department of Genetics, Faculty of Veterinary Medicine, Burdur Mehmet Akif Ersoy University, 15030, Burdur, Turkey

    Zafer Usta

  4. Department of Animal Nutrition and Nutritional Diseases, Faculty of Veterinary Medicine, Ankara University, 06830, Ankara, Turkey

    Gultekin Yildiz & Pinar Sacakli

  5. Department of Biochemistry, Faculty of Veterinary Medicine, Kafkas University, 36040, Kars, Turkey

    Oguz Merhan

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  1. Ozlem Durna Aydin
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Contributions

O.D.A., G.Y. and P.S. designed the research, and O.D.A., M.H., O.K. and O.M. conducted the experiments. M.H. and Z.U. analysed data, and O.D.A., M.H. and Z.U. wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Zafer Usta.

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Ethics approval

In 108 animals of Kars region in Turkey local goose breed, 3 groups (control, EK1 and EK2) will be studied, regardless of gender, with the samples obtained as a result of storing the liver samples taken in the study with the approval number 2018–54 of the Kafkas University Animal Experiments Local Ethics Committee. In addition, the authors complied with Directive 2010/63/EU.

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The authors declare no competing interests.

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Aydin, O.D., Hitit, M., Usta, Z. et al. Effects of essential oil mixtures on expression of genes involved in lipogenesis and fatty acid oxidation in geese (Anser anser). Trop Anim Health Prod 56, 30 (2024). https://doi.org/10.1007/s11250-023-03880-1

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