Abstract
Aims
Cadmium (Cd) is absorbed by plants into the food chain as a trace pollutant harmful to humans. The rice (Oryza sativa. L) glycosyltransferases (OsGTs) family is a large protein family with over hundreds of members, however, prior studies have failed to identify which OsGTs has a role in plants' response to Cd toxicity. In this study, we defined the role of OsGT17 in the regulation of grain Cd accumulation in rice.
Methods
One-week-old Zhonghua 11 (ZH11), osgt17 mutants, and OsGT17 overexpressing lines were treated with or without 1 μM Cd in solution for 7 days. Roots were then harvested for analysis of Cd as well as for cell wall and RNA extraction, while shoots were collected for Cd analysis.
Results
OsGT17 was located in the cell wall and displayed constitutive expression, despite its expression and protein accumulation were repressed by Cd. OsGT17 positively regulated Cd resistance in rice, as osgt17 mutants exhibited Cd sensitive phenotype while OsGT17 overexpressing plants exhibited Cd resistant phenotype. Furthermore, we showed that OsGT17 could regulate hemicellulose, especially xyloglucan level in root cell wall, which acted as a Cd compartmentalization sites, thus more Cd was accumulated in osgt17 mutants’ root cell wall and root, as a result, high levels of Cd were accumulated in osgt17 mutants’ shoots and grains that led them more sensitivity to Cd stress.
Conclusions
We first identified the role of a specific cell wall associated gene-OsGT17 in decreasing Cd accumulation in rice grains by fixing Cd in the root cell wall.
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Data availability
All data supporting the findings of this study are available within the paper and within its supplementary data published online.
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Funding
This study was supported by the National Natural Science Foundation of China (Grant No. 42020104004), the Field Frontier Program of the Institute of Soil Science (ISSASIP2215), the Agricultural Science and Technology Independent Innovation Fund Project of Jiangsu Province [CX(21)2034], the Key Research and Development Project of Jiangsu Province [BE2021717], and the Special Fund Project of Soil Pollution Prevention and Control [Xinyi Agriculture and Rural Bureau, New Collection List (2021) 1468].
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Tu, C.Y., Guo, R., Chen, C.Z. et al. Disruption of a glycosyl transferase family 17 protein alters cadmium accumulation and resistance in rice (Oryza sativa). Plant Soil (2024). https://doi.org/10.1007/s11104-024-06539-4
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DOI: https://doi.org/10.1007/s11104-024-06539-4