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MiR-29c Inhibits TNF-α-Induced ROS Production and Apoptosis in Mouse Hippocampal HT22 Cell Line

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Abstract

Recent reports have suggested that abnormal miR-29c expression in hippocampus have been implicated in the pathophysiology of some neurodegenerative and neuropsychiatric diseases. However, the underlying effect of miR-29c in regulating hippocampal neuronal function is not clear. In this study, HT22 cells were infected with lentivirus containing miR-29c or miR-29c sponge. Cell counting kit-8 (CCK8) and lactate dehydrogenase (LDH) assay kit were applied to evaluate cell viability and toxicity before and after TNF-α administration. Reactive oxygen species (ROS) generation and mitochondrial membrane potential (MMP) were measured with fluorescent probes. Hoechst 33258 staining and TUNEL assay were used to evaluate cell apoptosis. The expression of key mRNA/proteins (TNFR1, Bcl-2, Bax, TRADD, FADD, caspase-3, -8 and -9) in the apoptosis pathway was detected by PCR or WB. In addition, the protein expression of microtubule-associated protein-2 (MAP-2), nerve growth-associated protein 43 (GAP-43) and synapsin-1 (SYN-1) was detected by WB. As a result, we found that miR-29c overexpression could improve cell viability, attenuate LDH release, reduce ROS production and inhibit MMP depolarization in TNF-α-treated HT22 cells. Furthermore, miR-29c overexpression was found to decrease apoptotic rate, along with decreased expression of Bax, cleaved caspase-3, cleaved caspase-9, and increased expression of Bcl-2 in TNF-α-treated HT22 cells. However, miR-29c sponge exhibited an opposite effects. In addition, in TNF-α-treated HT22 cells, miR-29c overexpression could decrease the expressions of TNFR1, TRADD, FADD and cleaved caspase-8. However, in HT22 cells transfected with miR-29c sponge, TNF-α-induced the expressions of TNFR1, TRADD, FADD and cleaved caspase-8 was significantly exacerbated. At last, TNF-α-induced the decreased expression of MAP-2, GAP-43 and SYN-1 was reversed by miR-29c but exacerbated by miR-29c sponge. Overall, our study demonstrated that miR-29c protects against TNF-α-induced HT22 cells injury through alleviating ROS production and reduce neuronal apoptosis. Therefore, miR-29c might be a potential therapeutic agent for TNF-α accumulation and toxicity-related brain diseases.

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Data Availability

All data generated and analyzed during this study are available from the corresponding author on reasonable request.

Abbreviations

Bax:

Bcl-2 assaciated X protein

Bcl-2:

B-cell lymphoma/Leukemia-2

CCK8:

Cell counting kit-8

DHE:

Dihydroethidium

EGFP:

Enhanced green fluorescent protein

FADD:

Fas associated death domain protein

GAP-43:

Growth associated protein-43

iNOS:

Inducible nitric oxide synthase

LDH:

Lactate dehydrogenase

MAP-2:

Microtubule associated protein 2

MAP2K6:

Mitogen-Activated Protein Kinase Kinase 6

miRNAs:

microRNAs

MMP:

Mitochondrial membrane potential

ROS:

Reactive oxygen species

RTFQ-PCR:

Realtime Fluorescence Quantitative PCR

SYN-1:

Synaptophysin-1

TMRE:

Tetramethylrhodamine methyl ester

TNF-α:

Tumor necrosis factor-α

TNFR1:

Tumor necrosis factor receptor 1

TRADD:

TNFR-associated death domain protein

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31860291) and Major Research Project on Innovation Group of Education Department of Guizhou Province in 2018 (KY character in Guizhou Education Cooperation [2018]025). We are very grateful to the staff of the Department of Physiology and Key Laboratory of Infectious Diseases and Biosafety in Zunyi Medical University.

Funding

National Natural Science Foundation of China (No. 31860291). Major Research Project on Innovation Group of Education Department of Guizhou Province in 2018 (KY character in Guizhou Education Cooperation [2018]025).

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  1. Bo Li and Ying Lu have contributed equally to this work.

Authors and Affiliations

  1. Department of Physiology, Zunyi Medical University, Zunyi, 563000, Guizhou, China

    Bo Li, Ying Lu, Rong Wang, Tao Xu, Xiaolu Lei, Huan Jin, Xiaohong Gao, Ye Xie, Xiaohong Liu & Junwei Zeng

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Contributions

BL and YL drafted the manuscript. YL, XG and TX performed the immunofluorescence and analysis. BL, XL and HJ performed the RT-PCR and analysis. YL and RW performed the western blot and analysis. XL and JZ conceived of the study, participated in its design and coordination, and helped to draft the manuscript. All authors read and approved the final manuscript.

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Correspondence to Junwei Zeng.

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Li, B., Lu, Y., Wang, R. et al. MiR-29c Inhibits TNF-α-Induced ROS Production and Apoptosis in Mouse Hippocampal HT22 Cell Line. Neurochem Res 48, 519–536 (2023). https://doi.org/10.1007/s11064-022-03776-w

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